Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3279 and Shew_3280 are separated by 106 nucleotides Shew_3280 and rplI are separated by 77 nucleotides rplI and Shew_3282 are separated by 27 nucleotides Shew_3282 and Shew_3283 are separated by 12 nucleotides
Shew_3279: Shew_3279 - hypothetical protein (RefSeq), at 3,922,738 to 3,924,942
_3279
Shew_3280: Shew_3280 - hypothetical protein (RefSeq), at 3,925,049 to 3,925,486
_3280
Shew_3281: rplI - 50S ribosomal protein L9 (RefSeq), at 3,925,564 to 3,926,016
rplI
Shew_3282: Shew_3282 - ribosomal protein S18 (RefSeq), at 3,926,044 to 3,926,271
_3282
Shew_3283: Shew_3283 - single-strand binding protein/primosomal replication protein N (RefSeq), at 3,926,284 to 3,926,502
_3283
Position (kb)
3925
3926 Strain fitness (log2 ratio)
-1
0
1 at 3924.152 kb on + strand, within Shew_3279 at 3924.160 kb on - strand, within Shew_3279 at 3924.160 kb on - strand, within Shew_3279 at 3924.160 kb on - strand, within Shew_3279 at 3924.396 kb on + strand, within Shew_3279 at 3925.078 kb on - strand at 3925.151 kb on + strand, within Shew_3280 at 3925.159 kb on - strand, within Shew_3280 at 3925.229 kb on - strand, within Shew_3280 at 3925.302 kb on - strand, within Shew_3280 at 3925.365 kb on + strand, within Shew_3280 at 3925.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 3,924,152 + Shew_3279 0.64 -0.2 3,924,160 - Shew_3279 0.64 -0.7 3,924,160 - Shew_3279 0.64 +0.2 3,924,160 - Shew_3279 0.64 +0.5 3,924,396 + Shew_3279 0.75 -1.0 3,925,078 - +0.2 3,925,151 + Shew_3280 0.23 +0.4 3,925,159 - Shew_3280 0.25 +0.2 3,925,229 - Shew_3280 0.41 +0.0 3,925,302 - Shew_3280 0.58 +0.1 3,925,365 + Shew_3280 0.72 +1.1 3,925,458 - +0.3
Or see this region's nucleotide sequence