Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2547 and Shew_2548 are separated by 7 nucleotides Shew_2548 and Shew_2549 are separated by 5 nucleotides Shew_2549 and Shew_2550 are separated by 230 nucleotides
Shew_2547: Shew_2547 - pseudouridine synthase (RefSeq), at 3,003,986 to 3,004,849
_2547
Shew_2548: Shew_2548 - potassium efflux system protein (RefSeq), at 3,004,857 to 3,006,845
_2548
Shew_2549: Shew_2549 - NAD(P)H dehydrogenase (quinone) (RefSeq), at 3,006,851 to 3,007,468
_2549
Shew_2550: Shew_2550 - GCN5-related N-acetyltransferase (RefSeq), at 3,007,699 to 3,008,244
_2550
Position (kb)
3004
3005
3006
3007 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3004.002 kb on + strand at 3004.242 kb on - strand, within Shew_2547 at 3004.242 kb on - strand, within Shew_2547 at 3004.394 kb on + strand, within Shew_2547 at 3004.394 kb on + strand, within Shew_2547 at 3004.545 kb on + strand, within Shew_2547 at 3005.117 kb on + strand, within Shew_2548 at 3005.149 kb on - strand, within Shew_2548 at 3005.512 kb on + strand, within Shew_2548 at 3005.515 kb on - strand, within Shew_2548 at 3005.738 kb on + strand, within Shew_2548 at 3005.796 kb on - strand, within Shew_2548 at 3005.798 kb on + strand, within Shew_2548 at 3006.486 kb on + strand, within Shew_2548 at 3006.936 kb on + strand, within Shew_2549 at 3007.225 kb on + strand, within Shew_2549
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 3,004,002 + +0.1 3,004,242 - Shew_2547 0.30 -0.2 3,004,242 - Shew_2547 0.30 -3.1 3,004,394 + Shew_2547 0.47 -0.3 3,004,394 + Shew_2547 0.47 +0.5 3,004,545 + Shew_2547 0.65 -1.3 3,005,117 + Shew_2548 0.13 -1.9 3,005,149 - Shew_2548 0.15 -3.8 3,005,512 + Shew_2548 0.33 -3.7 3,005,515 - Shew_2548 0.33 -3.1 3,005,738 + Shew_2548 0.44 -1.3 3,005,796 - Shew_2548 0.47 -6.0 3,005,798 + Shew_2548 0.47 -3.1 3,006,486 + Shew_2548 0.82 -2.6 3,006,936 + Shew_2549 0.14 -3.5 3,007,225 + Shew_2549 0.61 -5.5
Or see this region's nucleotide sequence