Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2202 and Shew_2203 overlap by 19 nucleotides Shew_2203 and Shew_2204 are separated by 28 nucleotides Shew_2204 and Shew_2205 are separated by 365 nucleotides
Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436
_2202
Shew_2203: Shew_2203 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,563,418 to 2,564,191
_2203
Shew_2204: Shew_2204 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,564,220 to 2,564,840
_2204
Shew_2205: Shew_2205 - diguanylate cyclase (RefSeq), at 2,565,206 to 2,566,408
_2205
Position (kb)
2564
2565 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2563.591 kb on - strand, within Shew_2203 at 2563.902 kb on + strand, within Shew_2203 at 2563.973 kb on - strand, within Shew_2203 at 2564.354 kb on - strand, within Shew_2204 at 2565.269 kb on + strand at 2565.506 kb on + strand, within Shew_2205 at 2565.654 kb on - strand, within Shew_2205 at 2565.654 kb on - strand, within Shew_2205
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 2,563,591 - Shew_2203 0.22 -0.2 2,563,902 + Shew_2203 0.63 -0.2 2,563,973 - Shew_2203 0.72 -3.4 2,564,354 - Shew_2204 0.22 -1.0 2,565,269 + -1.0 2,565,506 + Shew_2205 0.25 +0.9 2,565,654 - Shew_2205 0.37 -1.5 2,565,654 - Shew_2205 0.37 +0.3
Or see this region's nucleotide sequence