Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1983 and Shew_1984 are separated by 192 nucleotides Shew_1984 and Shew_1985 are separated by 264 nucleotides Shew_1985 and Shew_1986 are separated by 12 nucleotides Shew_1986 and Shew_1987 are separated by 15 nucleotides Shew_1987 and Shew_1988 overlap by 1 nucleotides
Shew_1983: Shew_1983 - response regulator receiver modulated metal dependent phosphohydrolase (RefSeq), at 2,295,234 to 2,296,805
_1983
Shew_1984: Shew_1984 - hypothetical protein (RefSeq), at 2,296,998 to 2,297,495
_1984
Shew_1985: Shew_1985 - cytochrome c oxidase, cbb3-type, subunit I (RefSeq), at 2,297,760 to 2,299,187
_1985
Shew_1986: Shew_1986 - cytochrome c oxidase, cbb3-type, subunit II (RefSeq), at 2,299,200 to 2,299,805
_1986
Shew_1987: Shew_1987 - Cbb3-type cytochrome oxidase component (RefSeq), at 2,299,821 to 2,299,988
_1987
Shew_1988: Shew_1988 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,299,988 to 2,300,959
_1988
Position (kb)
2297
2298
2299
2300 Strain fitness (log2 ratio)
-1
0
1
2 at 2296.764 kb on - strand at 2296.927 kb on + strand at 2296.940 kb on + strand at 2296.967 kb on - strand at 2298.285 kb on + strand, within Shew_1985 at 2298.326 kb on + strand, within Shew_1985 at 2298.387 kb on - strand, within Shew_1985 at 2298.539 kb on - strand, within Shew_1985 at 2298.906 kb on - strand, within Shew_1985 at 2299.292 kb on - strand, within Shew_1986 at 2299.892 kb on + strand, within Shew_1987 at 2299.982 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 2,296,764 - -0.0 2,296,927 + -0.3 2,296,940 + -0.2 2,296,967 - -0.4 2,298,285 + Shew_1985 0.37 +1.7 2,298,326 + Shew_1985 0.40 +1.9 2,298,387 - Shew_1985 0.44 +2.7 2,298,539 - Shew_1985 0.55 +2.1 2,298,906 - Shew_1985 0.80 +1.3 2,299,292 - Shew_1986 0.15 +1.1 2,299,892 + Shew_1987 0.42 +1.5 2,299,982 - +0.6
Or see this region's nucleotide sequence