Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1563 and Shew_1564 are separated by 125 nucleotides Shew_1564 and Shew_1565 are separated by 244 nucleotides Shew_1565 and Shew_1566 are separated by 35 nucleotides Shew_1566 and Shew_1567 are separated by 219 nucleotides Shew_1567 and Shew_1568 are separated by 130 nucleotides
Shew_1563: Shew_1563 - isocitrate dehydrogenase, NADP-dependent (RefSeq), at 1,793,391 to 1,795,610
_1563
Shew_1564: Shew_1564 - cold-shock DNA-binding domain-containing protein (RefSeq), at 1,795,736 to 1,795,942
_1564
Shew_1565: Shew_1565 - ATP-dependent Clp protease adaptor protein ClpS (RefSeq), at 1,796,187 to 1,796,495
_1565
Shew_1566: Shew_1566 - ATP-dependent Clp protease, ATP-binding subunit clpA (RefSeq), at 1,796,531 to 1,798,789
_1566
Shew_1567: Shew_1567 - translation initiation factor IF-1 (RefSeq), at 1,799,009 to 1,799,227
_1567
Shew_1568: Shew_1568 - arginyl-tRNA-protein transferase (RefSeq), at 1,799,358 to 1,800,062
_1568
Position (kb)
1796
1797
1798
1799 Strain fitness (log2 ratio)
-2
-1
0
1 at 1795.783 kb on + strand, within Shew_1564 at 1795.807 kb on + strand, within Shew_1564 at 1796.125 kb on + strand at 1796.133 kb on - strand at 1796.466 kb on + strand at 1796.474 kb on + strand at 1797.443 kb on - strand, within Shew_1566 at 1797.480 kb on + strand, within Shew_1566 at 1797.916 kb on + strand, within Shew_1566 at 1798.105 kb on + strand, within Shew_1566 at 1798.107 kb on + strand, within Shew_1566 at 1799.535 kb on - strand, within Shew_1568 at 1799.535 kb on - strand, within Shew_1568 at 1799.644 kb on - strand, within Shew_1568 at 1799.763 kb on + strand, within Shew_1568
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 1,795,783 + Shew_1564 0.23 -0.5 1,795,807 + Shew_1564 0.34 -2.6 1,796,125 + -0.7 1,796,133 - -1.1 1,796,466 + -1.4 1,796,474 + -0.8 1,797,443 - Shew_1566 0.40 -1.3 1,797,480 + Shew_1566 0.42 -0.1 1,797,916 + Shew_1566 0.61 -2.1 1,798,105 + Shew_1566 0.70 -0.5 1,798,107 + Shew_1566 0.70 -2.0 1,799,535 - Shew_1568 0.25 +0.1 1,799,535 - Shew_1568 0.25 -0.3 1,799,644 - Shew_1568 0.41 +0.0 1,799,763 + Shew_1568 0.57 -0.2
Or see this region's nucleotide sequence