Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1226 and Shew_1227 are separated by 198 nucleotides Shew_1227 and Shew_1228 are separated by 2 nucleotides Shew_1228 and Shew_1229 are separated by 98 nucleotides
Shew_1226: Shew_1226 - peptidase M16 domain-containing protein (RefSeq), at 1,423,434 to 1,426,268
_1226
Shew_1227: Shew_1227 - glutamate--cysteine ligase (RefSeq), at 1,426,467 to 1,428,038
_1227
Shew_1228: Shew_1228 - hypothetical protein (RefSeq), at 1,428,041 to 1,428,481
_1228
Shew_1229: Shew_1229 - hypothetical protein (RefSeq), at 1,428,580 to 1,429,197
_1229
Position (kb)
1426
1427
1428
1429 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1425.544 kb on + strand, within Shew_1226 at 1425.562 kb on - strand, within Shew_1226 at 1425.677 kb on + strand, within Shew_1226 at 1425.766 kb on + strand, within Shew_1226 at 1425.824 kb on + strand, within Shew_1226 at 1425.886 kb on - strand, within Shew_1226 at 1425.944 kb on + strand, within Shew_1226 at 1426.008 kb on - strand at 1426.153 kb on - strand at 1426.241 kb on - strand at 1426.602 kb on + strand at 1426.706 kb on + strand, within Shew_1227 at 1427.665 kb on - strand, within Shew_1227 at 1428.879 kb on + strand, within Shew_1229
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 1,425,544 + Shew_1226 0.74 +0.5 1,425,562 - Shew_1226 0.75 -1.4 1,425,677 + Shew_1226 0.79 +0.9 1,425,766 + Shew_1226 0.82 +0.1 1,425,824 + Shew_1226 0.84 -0.6 1,425,886 - Shew_1226 0.86 +0.1 1,425,944 + Shew_1226 0.89 +0.6 1,426,008 - +0.2 1,426,153 - +0.2 1,426,241 - +1.4 1,426,602 + -3.5 1,426,706 + Shew_1227 0.15 -4.0 1,427,665 - Shew_1227 0.76 -3.4 1,428,879 + Shew_1229 0.48 +2.9
Or see this region's nucleotide sequence