Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1016 and Shew_1017 overlap by 8 nucleotides Shew_1017 and Shew_1018 are separated by 128 nucleotides Shew_1018 and Shew_1019 are separated by 60 nucleotides Shew_1019 and anmK are separated by 142 nucleotides
Shew_1016: Shew_1016 - hypothetical protein (RefSeq), at 1,176,212 to 1,177,129
_1016
Shew_1017: Shew_1017 - iron-sulfur cluster insertion protein ErpA (RefSeq), at 1,177,122 to 1,177,472
_1017
Shew_1018: Shew_1018 - hypothetical protein (RefSeq), at 1,177,601 to 1,178,074
_1018
Shew_1019: Shew_1019 - hypothetical protein (RefSeq), at 1,178,135 to 1,178,521
_1019
Shew_1020: anmK - anhydro-N-acetylmuramic acid kinase (RefSeq), at 1,178,664 to 1,179,773
anmK
Position (kb)
1177
1178
1179 Strain fitness (log2 ratio)
-1
0
1 at 1176.829 kb on + strand, within Shew_1016 at 1176.854 kb on - strand, within Shew_1016 at 1176.895 kb on - strand, within Shew_1016 at 1176.983 kb on - strand, within Shew_1016 at 1177.713 kb on - strand, within Shew_1018 at 1177.744 kb on + strand, within Shew_1018 at 1178.060 kb on - strand at 1178.154 kb on - strand at 1178.298 kb on - strand, within Shew_1019 at 1178.438 kb on - strand, within Shew_1019 at 1178.576 kb on - strand at 1178.603 kb on + strand at 1178.644 kb on + strand at 1178.841 kb on - strand, within anmK at 1178.894 kb on + strand, within anmK at 1179.022 kb on - strand, within anmK
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 1,176,829 + Shew_1016 0.67 -0.0 1,176,854 - Shew_1016 0.70 +0.2 1,176,895 - Shew_1016 0.74 +0.1 1,176,983 - Shew_1016 0.84 +0.4 1,177,713 - Shew_1018 0.24 +0.6 1,177,744 + Shew_1018 0.30 +0.0 1,178,060 - -1.5 1,178,154 - -1.0 1,178,298 - Shew_1019 0.42 +0.0 1,178,438 - Shew_1019 0.78 -0.8 1,178,576 - -0.2 1,178,603 + -0.2 1,178,644 + -0.4 1,178,841 - anmK Shew_1020 0.16 +0.7 1,178,894 + anmK Shew_1020 0.21 +0.7 1,179,022 - anmK Shew_1020 0.32 +0.8
Or see this region's nucleotide sequence