Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0517 and Shew_0518 are separated by 63 nucleotides Shew_0518 and Shew_0519 are separated by 78 nucleotides Shew_0519 and Shew_0520 are separated by 224 nucleotides
Shew_0517: Shew_0517 - putative plasmid transfer protein (RefSeq), at 586,558 to 587,754
_0517
Shew_0518: Shew_0518 - hypothetical protein (RefSeq), at 587,818 to 588,558
_0518
Shew_0519: Shew_0519 - transcriptional repressor protein MetJ (RefSeq), at 588,637 to 588,951
_0519
Shew_0520: Shew_0520 - O-succinylhomoserine (thiol)-lyase (RefSeq), at 589,176 to 590,342
_0520
Position (kb) 587
588
589 Strain fitness (log2 ratio) -1
0
1
2
3 at 586.966 kb on + strand, within Shew_0517 at 587.067 kb on - strand, within Shew_0517 at 587.146 kb on - strand, within Shew_0517 at 587.231 kb on + strand, within Shew_0517 at 587.357 kb on + strand, within Shew_0517 at 587.375 kb on - strand, within Shew_0517 at 587.505 kb on - strand, within Shew_0517 at 587.555 kb on - strand, within Shew_0517 at 587.810 kb on - strand at 587.875 kb on - strand at 587.944 kb on - strand, within Shew_0518 at 587.944 kb on - strand, within Shew_0518 at 587.975 kb on - strand, within Shew_0518 at 587.985 kb on + strand, within Shew_0518 at 588.520 kb on - strand at 588.541 kb on + strand at 588.809 kb on + strand, within Shew_0519 at 588.955 kb on - strand at 588.955 kb on - strand at 589.201 kb on + strand at 589.444 kb on - strand, within Shew_0520
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 586,966 + Shew_0517 0.34 -0.1 587,067 - Shew_0517 0.43 -0.2 587,146 - Shew_0517 0.49 -0.4 587,231 + Shew_0517 0.56 -0.1 587,357 + Shew_0517 0.67 -0.3 587,375 - Shew_0517 0.68 +0.4 587,505 - Shew_0517 0.79 -0.0 587,555 - Shew_0517 0.83 +0.4 587,810 - -0.2 587,875 - +0.5 587,944 - Shew_0518 0.17 -0.7 587,944 - Shew_0518 0.17 -1.6 587,975 - Shew_0518 0.21 -1.5 587,985 + Shew_0518 0.23 -0.8 588,520 - +0.1 588,541 + +1.0 588,809 + Shew_0519 0.55 +0.2 588,955 - +1.2 588,955 - +0.8 589,201 + +3.4 589,444 - Shew_0520 0.23 -0.5
Or see this region's nucleotide sequence