Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0127 and Shew_0128 are separated by 118 nucleotides Shew_0128 and nudE are separated by 97 nucleotides nudE and Shew_0130 are separated by 53 nucleotides Shew_0130 and Shew_0131 overlap by 8 nucleotides
Shew_0127: Shew_0127 - LexA repressor (RefSeq), at 137,523 to 138,140
_0127
Shew_0128: Shew_0128 - HAD family hydrolase (RefSeq), at 138,259 to 138,933
_0128
Shew_0129: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 139,031 to 139,576
nudE
Shew_0130: Shew_0130 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 139,630 to 140,442
_0130
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Position (kb)
139
140 Strain fitness (log2 ratio)
-2
-1
0
1 at 138.203 kb on - strand at 138.265 kb on - strand at 138.266 kb on + strand at 138.274 kb on - strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.276 kb on + strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.284 kb on - strand at 138.660 kb on - strand, within Shew_0128 at 138.660 kb on - strand, within Shew_0128 at 138.829 kb on + strand, within Shew_0128 at 138.843 kb on + strand, within Shew_0128 at 139.081 kb on - strand at 139.161 kb on - strand, within nudE at 139.239 kb on + strand, within nudE at 139.310 kb on - strand, within nudE at 139.326 kb on + strand, within nudE at 139.367 kb on - strand, within nudE at 139.488 kb on + strand, within nudE at 139.642 kb on - strand at 139.917 kb on - strand, within Shew_0130 at 140.097 kb on - strand, within Shew_0130 at 140.201 kb on - strand, within Shew_0130 at 140.442 kb on + strand at 140.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 138,203 - +0.7 138,265 - +0.4 138,266 + +0.1 138,274 - +0.4 138,276 + -1.0 138,276 + +1.2 138,276 + -0.0 138,276 + -0.1 138,276 + -1.4 138,276 + +0.6 138,276 + +0.0 138,276 + -0.0 138,276 + +0.2 138,276 + +0.3 138,284 - -0.2 138,284 - +0.1 138,284 - +0.8 138,284 - +0.9 138,284 - +0.1 138,284 - -0.0 138,284 - -0.3 138,284 - -0.3 138,284 - -0.2 138,660 - Shew_0128 0.59 +0.2 138,660 - Shew_0128 0.59 -0.1 138,829 + Shew_0128 0.84 +0.3 138,843 + Shew_0128 0.87 -0.0 139,081 - +0.0 139,161 - nudE Shew_0129 0.24 -0.7 139,239 + nudE Shew_0129 0.38 -1.9 139,310 - nudE Shew_0129 0.51 +0.0 139,326 + nudE Shew_0129 0.54 -1.7 139,367 - nudE Shew_0129 0.62 -0.6 139,488 + nudE Shew_0129 0.84 -0.4 139,642 - +0.1 139,917 - Shew_0130 0.35 +0.7 140,097 - Shew_0130 0.57 +0.4 140,201 - Shew_0130 0.70 -1.5 140,442 + +0.1 140,476 - +0.6
Or see this region's nucleotide sequence