Experiment: MoLS4 with L-Glutamate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifN and nifE overlap by 14 nucleotides nifE and DVUA0010 are separated by 642 nucleotides
DVUA0008: nifN - nitrogenase molybdenum-iron cofactor biosynthesis protein NifN, putative (TIGR), at 10,458 to 11,972
nifN
DVUA0009: nifE - nitrogenase MoFe cofactor biosynthesis protein NifE domain protein (TIGR), at 11,959 to 13,665
nifE
DVUA0010: DVUA0010 - ferredoxin, 2fe-2s (TIGR), at 14,308 to 14,610
DVUA0010
Position (kb) 11
12
13
14 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 10.997 kb on + strand, within nifN at 11.011 kb on + strand, within nifN at 11.037 kb on - strand, within nifN at 11.038 kb on + strand, within nifN at 11.041 kb on - strand, within nifN at 11.284 kb on - strand, within nifN at 11.293 kb on + strand, within nifN at 11.293 kb on + strand, within nifN at 11.327 kb on - strand, within nifN at 11.338 kb on + strand, within nifN at 11.347 kb on - strand, within nifN at 11.382 kb on - strand, within nifN at 11.394 kb on + strand, within nifN at 11.417 kb on - strand, within nifN at 11.455 kb on + strand, within nifN at 11.458 kb on - strand, within nifN at 11.479 kb on + strand, within nifN at 11.505 kb on - strand, within nifN at 11.536 kb on + strand, within nifN at 11.536 kb on + strand, within nifN at 11.536 kb on + strand, within nifN at 11.544 kb on - strand, within nifN at 11.570 kb on + strand, within nifN at 11.593 kb on + strand, within nifN at 11.683 kb on - strand, within nifN at 11.687 kb on - strand, within nifN at 11.730 kb on - strand, within nifN at 11.772 kb on + strand, within nifN at 11.791 kb on - strand, within nifN at 11.799 kb on + strand, within nifN at 11.799 kb on + strand, within nifN at 11.799 kb on + strand, within nifN at 11.807 kb on - strand, within nifN at 11.807 kb on - strand, within nifN at 11.807 kb on - strand, within nifN at 11.838 kb on + strand at 11.846 kb on - strand at 11.855 kb on + strand at 11.901 kb on + strand at 11.919 kb on - strand at 11.974 kb on - strand at 11.976 kb on - strand at 12.076 kb on + strand at 12.144 kb on - strand, within nifE at 12.163 kb on + strand, within nifE at 12.192 kb on + strand, within nifE at 12.202 kb on + strand, within nifE at 12.210 kb on + strand, within nifE at 12.313 kb on + strand, within nifE at 12.321 kb on - strand, within nifE at 12.340 kb on - strand, within nifE at 12.342 kb on - strand, within nifE at 12.360 kb on + strand, within nifE at 12.372 kb on - strand, within nifE at 12.488 kb on - strand, within nifE at 12.488 kb on - strand, within nifE at 12.550 kb on - strand, within nifE at 12.705 kb on - strand, within nifE at 12.764 kb on + strand, within nifE at 12.834 kb on + strand, within nifE at 12.842 kb on - strand, within nifE at 12.852 kb on - strand, within nifE at 12.877 kb on + strand, within nifE at 12.895 kb on - strand, within nifE at 12.897 kb on + strand, within nifE at 12.910 kb on - strand, within nifE at 13.014 kb on + strand, within nifE at 13.017 kb on - strand, within nifE at 13.033 kb on - strand, within nifE at 13.067 kb on - strand, within nifE at 13.080 kb on - strand, within nifE at 13.096 kb on + strand, within nifE at 13.123 kb on - strand, within nifE at 13.124 kb on - strand, within nifE at 13.204 kb on - strand, within nifE at 13.252 kb on + strand, within nifE at 13.260 kb on - strand, within nifE at 13.270 kb on - strand, within nifE at 13.363 kb on - strand, within nifE at 13.367 kb on - strand, within nifE at 13.369 kb on + strand, within nifE at 13.369 kb on + strand, within nifE at 13.374 kb on + strand, within nifE at 13.374 kb on + strand, within nifE at 13.374 kb on + strand, within nifE at 13.377 kb on - strand, within nifE at 13.382 kb on - strand, within nifE at 13.394 kb on + strand, within nifE at 13.411 kb on + strand, within nifE at 13.428 kb on + strand, within nifE at 13.466 kb on + strand, within nifE at 13.466 kb on + strand, within nifE at 13.468 kb on + strand, within nifE at 13.471 kb on - strand, within nifE at 13.474 kb on - strand, within nifE at 13.474 kb on - strand, within nifE at 13.513 kb on - strand at 13.566 kb on - strand at 13.581 kb on - strand at 13.733 kb on + strand at 13.774 kb on + strand at 13.819 kb on + strand at 13.826 kb on + strand at 13.863 kb on - strand at 13.900 kb on + strand at 13.939 kb on + strand at 13.944 kb on + strand at 13.947 kb on - strand at 13.947 kb on - strand at 13.947 kb on - strand at 13.958 kb on + strand at 14.001 kb on + strand at 14.023 kb on + strand at 14.045 kb on - strand at 14.069 kb on + strand at 14.069 kb on + strand at 14.077 kb on - strand at 14.077 kb on - strand at 14.082 kb on - strand at 14.082 kb on - strand at 14.082 kb on - strand at 14.106 kb on + strand at 14.109 kb on - strand at 14.325 kb on - strand at 14.330 kb on - strand at 14.509 kb on - strand, within DVUA0010 at 14.600 kb on + strand at 14.619 kb on + strand at 14.659 kb on - strand at 14.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamate (N) remove 10,997 + nifN DVUA0008 0.36 -2.6 11,011 + nifN DVUA0008 0.37 -1.5 11,037 - nifN DVUA0008 0.38 -2.3 11,038 + nifN DVUA0008 0.38 -2.8 11,041 - nifN DVUA0008 0.38 -2.8 11,284 - nifN DVUA0008 0.55 -3.4 11,293 + nifN DVUA0008 0.55 -3.2 11,293 + nifN DVUA0008 0.55 -2.9 11,327 - nifN DVUA0008 0.57 -1.2 11,338 + nifN DVUA0008 0.58 -1.9 11,347 - nifN DVUA0008 0.59 -3.5 11,382 - nifN DVUA0008 0.61 -3.5 11,394 + nifN DVUA0008 0.62 -3.4 11,417 - nifN DVUA0008 0.63 -2.9 11,455 + nifN DVUA0008 0.66 -2.8 11,458 - nifN DVUA0008 0.66 -1.2 11,479 + nifN DVUA0008 0.67 -2.6 11,505 - nifN DVUA0008 0.69 -1.8 11,536 + nifN DVUA0008 0.71 -1.7 11,536 + nifN DVUA0008 0.71 -2.6 11,536 + nifN DVUA0008 0.71 -2.8 11,544 - nifN DVUA0008 0.72 -2.9 11,570 + nifN DVUA0008 0.73 -1.7 11,593 + nifN DVUA0008 0.75 -2.9 11,683 - nifN DVUA0008 0.81 -2.9 11,687 - nifN DVUA0008 0.81 -2.1 11,730 - nifN DVUA0008 0.84 -2.4 11,772 + nifN DVUA0008 0.87 -3.9 11,791 - nifN DVUA0008 0.88 -3.0 11,799 + nifN DVUA0008 0.89 -3.1 11,799 + nifN DVUA0008 0.89 -2.1 11,799 + nifN DVUA0008 0.89 -0.3 11,807 - nifN DVUA0008 0.89 -3.9 11,807 - nifN DVUA0008 0.89 -3.0 11,807 - nifN DVUA0008 0.89 -2.7 11,838 + -2.2 11,846 - -3.4 11,855 + -2.6 11,901 + -1.7 11,919 - -2.3 11,974 - -2.7 11,976 - -1.1 12,076 + -1.4 12,144 - nifE DVUA0009 0.11 -0.7 12,163 + nifE DVUA0009 0.12 -1.3 12,192 + nifE DVUA0009 0.14 -1.6 12,202 + nifE DVUA0009 0.14 -0.5 12,210 + nifE DVUA0009 0.15 -2.4 12,313 + nifE DVUA0009 0.21 -4.0 12,321 - nifE DVUA0009 0.21 -3.0 12,340 - nifE DVUA0009 0.22 -1.5 12,342 - nifE DVUA0009 0.22 -3.8 12,360 + nifE DVUA0009 0.23 -1.5 12,372 - nifE DVUA0009 0.24 -2.8 12,488 - nifE DVUA0009 0.31 -1.7 12,488 - nifE DVUA0009 0.31 -3.3 12,550 - nifE DVUA0009 0.35 -3.9 12,705 - nifE DVUA0009 0.44 -2.7 12,764 + nifE DVUA0009 0.47 -2.9 12,834 + nifE DVUA0009 0.51 -0.5 12,842 - nifE DVUA0009 0.52 -0.8 12,852 - nifE DVUA0009 0.52 -3.7 12,877 + nifE DVUA0009 0.54 -2.6 12,895 - nifE DVUA0009 0.55 -2.2 12,897 + nifE DVUA0009 0.55 -0.5 12,910 - nifE DVUA0009 0.56 -3.3 13,014 + nifE DVUA0009 0.62 -2.6 13,017 - nifE DVUA0009 0.62 -2.1 13,033 - nifE DVUA0009 0.63 -2.8 13,067 - nifE DVUA0009 0.65 -3.5 13,080 - nifE DVUA0009 0.66 -0.7 13,096 + nifE DVUA0009 0.67 -1.2 13,123 - nifE DVUA0009 0.68 -2.4 13,124 - nifE DVUA0009 0.68 -2.5 13,204 - nifE DVUA0009 0.73 -3.1 13,252 + nifE DVUA0009 0.76 -3.0 13,260 - nifE DVUA0009 0.76 -3.0 13,270 - nifE DVUA0009 0.77 -1.2 13,363 - nifE DVUA0009 0.82 -3.1 13,367 - nifE DVUA0009 0.82 -1.9 13,369 + nifE DVUA0009 0.83 -2.3 13,369 + nifE DVUA0009 0.83 -3.3 13,374 + nifE DVUA0009 0.83 -2.5 13,374 + nifE DVUA0009 0.83 -1.4 13,374 + nifE DVUA0009 0.83 -2.6 13,377 - nifE DVUA0009 0.83 -2.9 13,382 - nifE DVUA0009 0.83 -2.2 13,394 + nifE DVUA0009 0.84 -1.7 13,411 + nifE DVUA0009 0.85 -1.6 13,428 + nifE DVUA0009 0.86 -3.5 13,466 + nifE DVUA0009 0.88 -0.9 13,466 + nifE DVUA0009 0.88 -2.4 13,468 + nifE DVUA0009 0.88 -3.3 13,471 - nifE DVUA0009 0.89 -2.7 13,474 - nifE DVUA0009 0.89 -1.9 13,474 - nifE DVUA0009 0.89 -1.0 13,513 - -2.2 13,566 - -2.7 13,581 - -2.2 13,733 + -1.6 13,774 + -3.1 13,819 + -3.0 13,826 + -1.5 13,863 - -0.6 13,900 + -1.6 13,939 + +1.0 13,944 + -1.2 13,947 - -0.3 13,947 - -0.7 13,947 - -0.3 13,958 + -1.0 14,001 + -0.6 14,023 + -1.0 14,045 - -0.3 14,069 + -1.2 14,069 + -0.6 14,077 - -0.2 14,077 - +0.4 14,082 - +0.4 14,082 - -0.3 14,082 - -1.6 14,106 + -0.5 14,109 - -0.2 14,325 - +0.1 14,330 - -0.8 14,509 - DVUA0010 0.66 -0.9 14,600 + -1.1 14,619 + -0.6 14,659 - +1.3 14,665 + -0.2
Or see this region's nucleotide sequence