Experiment: MoLS4 with L-Glutamate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuA and leuC are separated by 1 nucleotides leuC and leuD are separated by 17 nucleotides leuD and DVU2984 are separated by 2 nucleotides
DVU2981: leuA - 2-isopropylmalate synthase (TIGR), at 3,087,841 to 3,089,370
leuA
DVU2982: leuC - 3-isopropylmalate dehydratase / (R)-citramalate isomerase, large subunit LeuC (EC 4.2.1.33; EC 4.2.1.35) (from data) , at 3,089,372 to 3,090,631
leuC
DVU2983: leuD - 3-isopropylmalate dehydratase / (R)-citramalate isomerase, small subunit LeuD (EC 4.2.1.33; EC 4.2.1.35) (from data) , at 3,090,649 to 3,091,152
leuD
DVU2984: DVU2984 - conserved hypothetical protein (TIGR), at 3,091,155 to 3,092,162
DVU2984
Position (kb)
3089
3090
3091 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3088.482 kb on + strand, within leuA at 3088.572 kb on + strand, within leuA at 3088.580 kb on - strand, within leuA at 3088.580 kb on - strand, within leuA at 3088.689 kb on + strand, within leuA at 3088.738 kb on + strand, within leuA at 3088.746 kb on - strand, within leuA at 3088.746 kb on - strand, within leuA at 3088.761 kb on - strand, within leuA at 3088.867 kb on + strand, within leuA at 3088.875 kb on - strand, within leuA at 3088.880 kb on + strand, within leuA at 3088.888 kb on - strand, within leuA at 3088.896 kb on + strand, within leuA at 3088.896 kb on + strand, within leuA at 3088.896 kb on + strand, within leuA at 3088.896 kb on + strand, within leuA at 3088.904 kb on - strand, within leuA at 3088.904 kb on - strand, within leuA at 3088.904 kb on - strand, within leuA at 3088.904 kb on - strand, within leuA at 3088.908 kb on + strand, within leuA at 3088.914 kb on - strand, within leuA at 3088.967 kb on + strand, within leuA at 3088.995 kb on - strand, within leuA at 3089.158 kb on - strand, within leuA at 3089.162 kb on + strand, within leuA at 3089.183 kb on - strand, within leuA at 3089.336 kb on - strand at 3089.337 kb on + strand at 3089.359 kb on - strand at 3089.375 kb on + strand at 3089.383 kb on - strand at 3089.383 kb on - strand at 3089.465 kb on + strand at 3089.465 kb on + strand at 3089.557 kb on + strand, within leuC at 3089.652 kb on + strand, within leuC at 3089.653 kb on - strand, within leuC at 3089.694 kb on - strand, within leuC at 3089.763 kb on + strand, within leuC at 3089.783 kb on + strand, within leuC at 3089.783 kb on + strand, within leuC at 3089.818 kb on - strand, within leuC at 3089.833 kb on + strand, within leuC at 3089.841 kb on - strand, within leuC at 3089.925 kb on - strand, within leuC at 3089.942 kb on + strand, within leuC at 3089.942 kb on + strand, within leuC at 3089.947 kb on + strand, within leuC at 3089.950 kb on - strand, within leuC at 3090.076 kb on + strand, within leuC at 3090.115 kb on + strand, within leuC at 3090.137 kb on + strand, within leuC at 3090.145 kb on - strand, within leuC at 3090.164 kb on - strand, within leuC at 3090.178 kb on - strand, within leuC at 3090.348 kb on + strand, within leuC at 3090.412 kb on - strand, within leuC at 3090.565 kb on + strand at 3090.616 kb on + strand at 3090.618 kb on + strand at 3090.624 kb on - strand at 3090.766 kb on - strand, within leuD at 3090.777 kb on + strand, within leuD at 3090.787 kb on - strand, within leuD at 3090.939 kb on - strand, within leuD at 3091.059 kb on - strand, within leuD at 3091.066 kb on + strand, within leuD at 3091.074 kb on - strand, within leuD at 3091.114 kb on + strand at 3091.269 kb on - strand, within DVU2984 at 3091.291 kb on - strand, within DVU2984 at 3091.355 kb on + strand, within DVU2984 at 3091.402 kb on - strand, within DVU2984 at 3091.464 kb on + strand, within DVU2984 at 3091.472 kb on - strand, within DVU2984 at 3091.504 kb on + strand, within DVU2984 at 3091.569 kb on + strand, within DVU2984
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamate (N) remove 3,088,482 + leuA DVU2981 0.42 -3.3 3,088,572 + leuA DVU2981 0.48 -3.8 3,088,580 - leuA DVU2981 0.48 -4.3 3,088,580 - leuA DVU2981 0.48 +0.4 3,088,689 + leuA DVU2981 0.55 -1.5 3,088,738 + leuA DVU2981 0.59 -3.2 3,088,746 - leuA DVU2981 0.59 -4.1 3,088,746 - leuA DVU2981 0.59 -4.3 3,088,761 - leuA DVU2981 0.60 -2.4 3,088,867 + leuA DVU2981 0.67 -2.4 3,088,875 - leuA DVU2981 0.68 -2.9 3,088,880 + leuA DVU2981 0.68 -2.9 3,088,888 - leuA DVU2981 0.68 -2.5 3,088,896 + leuA DVU2981 0.69 -2.4 3,088,896 + leuA DVU2981 0.69 -4.2 3,088,896 + leuA DVU2981 0.69 -4.3 3,088,896 + leuA DVU2981 0.69 -2.2 3,088,904 - leuA DVU2981 0.69 -2.4 3,088,904 - leuA DVU2981 0.69 -3.6 3,088,904 - leuA DVU2981 0.69 -3.1 3,088,904 - leuA DVU2981 0.69 -1.9 3,088,908 + leuA DVU2981 0.70 -1.4 3,088,914 - leuA DVU2981 0.70 -1.0 3,088,967 + leuA DVU2981 0.74 +0.1 3,088,995 - leuA DVU2981 0.75 -3.7 3,089,158 - leuA DVU2981 0.86 -3.1 3,089,162 + leuA DVU2981 0.86 -2.3 3,089,183 - leuA DVU2981 0.88 -0.9 3,089,336 - -0.6 3,089,337 + -0.3 3,089,359 - -0.5 3,089,375 + -2.2 3,089,383 - -2.5 3,089,383 - -3.3 3,089,465 + -4.3 3,089,465 + -4.1 3,089,557 + leuC DVU2982 0.15 -0.6 3,089,652 + leuC DVU2982 0.22 -1.3 3,089,653 - leuC DVU2982 0.22 -2.6 3,089,694 - leuC DVU2982 0.26 -1.2 3,089,763 + leuC DVU2982 0.31 -2.7 3,089,783 + leuC DVU2982 0.33 -3.2 3,089,783 + leuC DVU2982 0.33 -4.1 3,089,818 - leuC DVU2982 0.35 -0.6 3,089,833 + leuC DVU2982 0.37 -4.5 3,089,841 - leuC DVU2982 0.37 -3.6 3,089,925 - leuC DVU2982 0.44 -2.9 3,089,942 + leuC DVU2982 0.45 -2.4 3,089,942 + leuC DVU2982 0.45 -2.5 3,089,947 + leuC DVU2982 0.46 -5.1 3,089,950 - leuC DVU2982 0.46 -0.9 3,090,076 + leuC DVU2982 0.56 -2.6 3,090,115 + leuC DVU2982 0.59 -3.2 3,090,137 + leuC DVU2982 0.61 -2.8 3,090,145 - leuC DVU2982 0.61 -2.9 3,090,164 - leuC DVU2982 0.63 -4.2 3,090,178 - leuC DVU2982 0.64 -2.5 3,090,348 + leuC DVU2982 0.77 -3.3 3,090,412 - leuC DVU2982 0.83 -3.5 3,090,565 + -0.6 3,090,616 + +0.1 3,090,618 + -0.5 3,090,624 - -0.6 3,090,766 - leuD DVU2983 0.23 -5.6 3,090,777 + leuD DVU2983 0.25 -3.3 3,090,787 - leuD DVU2983 0.27 -4.2 3,090,939 - leuD DVU2983 0.58 -3.9 3,091,059 - leuD DVU2983 0.81 -3.4 3,091,066 + leuD DVU2983 0.83 -4.6 3,091,074 - leuD DVU2983 0.84 -2.0 3,091,114 + -0.6 3,091,269 - DVU2984 0.11 -1.0 3,091,291 - DVU2984 0.13 -0.9 3,091,355 + DVU2984 0.20 -1.2 3,091,402 - DVU2984 0.25 -0.9 3,091,464 + DVU2984 0.31 -1.2 3,091,472 - DVU2984 0.31 -0.6 3,091,504 + DVU2984 0.35 -0.1 3,091,569 + DVU2984 0.41 -1.6
Or see this region's nucleotide sequence