Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3569 and Rru_A3570 are separated by 63 nucleotides Rru_A3570 and Rru_A3571 are separated by 66 nucleotides Rru_A3571 and Rru_A3572 are separated by 263 nucleotides Rru_A3572 and Rru_A3573 are separated by 24 nucleotides Rru_A3573 and Rru_A3574 are separated by 3 nucleotides
Rru_A3569: Rru_A3569 - O-sialoglycoprotein endopeptidase (NCBI), at 4,114,465 to 4,115,454
_A3569
Rru_A3570: Rru_A3570 - Protein of unknown function DUF29 (NCBI), at 4,115,518 to 4,115,961
_A3570
Rru_A3571: Rru_A3571 - Glycerol-3-phosphate dehydrogenase (NAD(P)+) (NCBI), at 4,116,028 to 4,117,077
_A3571
Rru_A3572: Rru_A3572 - YCII-related (NCBI), at 4,117,341 to 4,117,631
_A3572
Rru_A3573: Rru_A3573 - Protein of unknown function DUF589 (NCBI), at 4,117,656 to 4,118,063
_A3573
Rru_A3574: Rru_A3574 - Rieske (2Fe-2S) region (NCBI), at 4,118,067 to 4,118,420
_A3574
Position (kb) 4116
4117
4118 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4115.444 kb on + strand at 4115.444 kb on + strand at 4115.452 kb on + strand at 4115.452 kb on + strand at 4115.455 kb on + strand at 4115.455 kb on + strand at 4115.455 kb on + strand at 4115.456 kb on - strand at 4115.456 kb on - strand at 4115.456 kb on - strand at 4115.456 kb on - strand at 4115.456 kb on - strand at 4115.480 kb on - strand at 4115.535 kb on - strand at 4115.537 kb on - strand at 4115.537 kb on - strand at 4115.537 kb on - strand at 4115.552 kb on - strand at 4115.879 kb on - strand, within Rru_A3570 at 4115.879 kb on - strand, within Rru_A3570 at 4115.903 kb on + strand, within Rru_A3570 at 4115.903 kb on + strand, within Rru_A3570 at 4115.903 kb on + strand, within Rru_A3570 at 4115.903 kb on + strand, within Rru_A3570 at 4115.903 kb on + strand, within Rru_A3570 at 4115.904 kb on - strand, within Rru_A3570 at 4115.904 kb on - strand, within Rru_A3570 at 4115.904 kb on - strand, within Rru_A3570 at 4115.904 kb on - strand, within Rru_A3570 at 4115.959 kb on + strand at 4115.960 kb on - strand at 4115.960 kb on - strand at 4115.960 kb on - strand at 4116.048 kb on + strand at 4116.048 kb on + strand at 4116.048 kb on + strand at 4116.048 kb on + strand at 4116.560 kb on - strand, within Rru_A3571 at 4116.885 kb on + strand, within Rru_A3571 at 4116.885 kb on + strand, within Rru_A3571 at 4116.886 kb on - strand, within Rru_A3571 at 4116.886 kb on - strand, within Rru_A3571 at 4116.973 kb on + strand at 4116.973 kb on + strand at 4116.973 kb on + strand at 4117.243 kb on + strand at 4117.243 kb on + strand at 4117.243 kb on + strand at 4117.244 kb on - strand at 4117.244 kb on - strand at 4117.271 kb on + strand at 4117.367 kb on + strand at 4117.367 kb on + strand at 4117.368 kb on - strand at 4117.419 kb on + strand, within Rru_A3572 at 4117.420 kb on - strand, within Rru_A3572 at 4117.557 kb on + strand, within Rru_A3572 at 4117.557 kb on + strand, within Rru_A3572 at 4117.558 kb on - strand, within Rru_A3572 at 4117.599 kb on + strand, within Rru_A3572 at 4117.599 kb on + strand, within Rru_A3572 at 4117.599 kb on + strand, within Rru_A3572 at 4117.600 kb on - strand, within Rru_A3572 at 4117.600 kb on - strand, within Rru_A3572 at 4117.601 kb on + strand, within Rru_A3572 at 4117.601 kb on + strand, within Rru_A3572 at 4117.641 kb on + strand at 4117.641 kb on + strand at 4117.641 kb on + strand at 4117.642 kb on - strand at 4117.642 kb on - strand at 4117.642 kb on - strand at 4117.857 kb on + strand, within Rru_A3573 at 4118.061 kb on + strand at 4118.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 4,115,444 + +0.0 4,115,444 + +0.0 4,115,452 + -1.0 4,115,452 + +0.0 4,115,455 + +0.0 4,115,455 + -1.0 4,115,455 + -1.3 4,115,456 - -1.8 4,115,456 - -1.8 4,115,456 - +0.0 4,115,456 - +0.0 4,115,456 - +0.0 4,115,480 - +0.0 4,115,535 - -1.0 4,115,537 - +0.0 4,115,537 - +0.0 4,115,537 - -0.6 4,115,552 - -1.0 4,115,879 - Rru_A3570 0.81 +0.0 4,115,879 - Rru_A3570 0.81 +0.0 4,115,903 + Rru_A3570 0.87 +0.0 4,115,903 + Rru_A3570 0.87 +0.0 4,115,903 + Rru_A3570 0.87 -0.8 4,115,903 + Rru_A3570 0.87 +0.0 4,115,903 + Rru_A3570 0.87 +0.0 4,115,904 - Rru_A3570 0.87 +0.0 4,115,904 - Rru_A3570 0.87 +0.0 4,115,904 - Rru_A3570 0.87 +0.4 4,115,904 - Rru_A3570 0.87 +0.0 4,115,959 + -0.6 4,115,960 - +0.0 4,115,960 - +0.0 4,115,960 - -0.4 4,116,048 + -3.6 4,116,048 + +0.0 4,116,048 + -2.4 4,116,048 + +0.0 4,116,560 - Rru_A3571 0.51 +0.1 4,116,885 + Rru_A3571 0.82 -3.9 4,116,885 + Rru_A3571 0.82 +0.1 4,116,886 - Rru_A3571 0.82 +0.1 4,116,886 - Rru_A3571 0.82 -3.3 4,116,973 + +0.1 4,116,973 + +0.1 4,116,973 + +0.1 4,117,243 + +0.1 4,117,243 + +0.1 4,117,243 + +0.1 4,117,244 - +0.1 4,117,244 - -0.4 4,117,271 + -1.1 4,117,367 + +0.1 4,117,367 + +0.1 4,117,368 - -1.5 4,117,419 + Rru_A3572 0.27 +0.1 4,117,420 - Rru_A3572 0.27 -1.5 4,117,557 + Rru_A3572 0.74 +0.1 4,117,557 + Rru_A3572 0.74 +0.1 4,117,558 - Rru_A3572 0.75 -1.3 4,117,599 + Rru_A3572 0.89 -1.2 4,117,599 + Rru_A3572 0.89 -1.5 4,117,599 + Rru_A3572 0.89 +0.1 4,117,600 - Rru_A3572 0.89 -2.1 4,117,600 - Rru_A3572 0.89 +0.1 4,117,601 + Rru_A3572 0.89 +0.1 4,117,601 + Rru_A3572 0.89 +0.1 4,117,641 + +0.1 4,117,641 + -2.1 4,117,641 + +0.1 4,117,642 - +0.1 4,117,642 - +0.1 4,117,642 - -1.5 4,117,857 + Rru_A3573 0.49 +0.1 4,118,061 + +0.1 4,118,061 + -2.5
Or see this region's nucleotide sequence