Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1305 and Rru_A1306 are separated by 19 nucleotides Rru_A1306 and Rru_A1307 overlap by 4 nucleotides Rru_A1307 and Rru_A1308 are separated by 108 nucleotides
Rru_A1305: Rru_A1305 - Predicted transcriptional regulator (NCBI), at 1,540,461 to 1,540,757
_A1305
Rru_A1306: Rru_A1306 - Short-chain dehydrogenase/reductase SDR (NCBI), at 1,540,777 to 1,541,610
_A1306
Rru_A1307: Rru_A1307 - Thioesterase superfamily (NCBI), at 1,541,607 to 1,542,119
_A1307
Rru_A1308: Rru_A1308 - Acyl-CoA dehydrogenase (NCBI), at 1,542,228 to 1,544,018
_A1308
Position (kb) 1541
1542
1543 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1540.691 kb on - strand, within Rru_A1305 at 1540.691 kb on - strand, within Rru_A1305 at 1540.691 kb on - strand, within Rru_A1305 at 1541.106 kb on + strand, within Rru_A1306 at 1541.106 kb on + strand, within Rru_A1306 at 1541.106 kb on + strand, within Rru_A1306 at 1541.106 kb on + strand, within Rru_A1306 at 1541.106 kb on + strand, within Rru_A1306 at 1541.107 kb on - strand, within Rru_A1306 at 1541.107 kb on - strand, within Rru_A1306 at 1541.169 kb on + strand, within Rru_A1306 at 1541.502 kb on + strand, within Rru_A1306 at 1541.503 kb on - strand, within Rru_A1306 at 1541.611 kb on - strand at 1541.708 kb on - strand, within Rru_A1307 at 1542.029 kb on + strand, within Rru_A1307 at 1542.029 kb on + strand, within Rru_A1307 at 1542.030 kb on - strand, within Rru_A1307 at 1542.030 kb on - strand, within Rru_A1307 at 1542.030 kb on - strand, within Rru_A1307 at 1542.030 kb on - strand, within Rru_A1307 at 1542.213 kb on + strand at 1542.396 kb on + strand at 1542.422 kb on - strand, within Rru_A1308 at 1542.435 kb on - strand, within Rru_A1308 at 1542.455 kb on - strand, within Rru_A1308 at 1542.601 kb on + strand, within Rru_A1308 at 1542.601 kb on + strand, within Rru_A1308 at 1542.601 kb on + strand, within Rru_A1308 at 1542.601 kb on + strand, within Rru_A1308 at 1542.703 kb on + strand, within Rru_A1308 at 1542.704 kb on - strand, within Rru_A1308 at 1542.704 kb on - strand, within Rru_A1308 at 1542.733 kb on + strand, within Rru_A1308 at 1542.733 kb on + strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.734 kb on - strand, within Rru_A1308 at 1542.760 kb on + strand, within Rru_A1308 at 1542.760 kb on + strand, within Rru_A1308 at 1542.761 kb on - strand, within Rru_A1308 at 1542.761 kb on - strand, within Rru_A1308 at 1543.072 kb on + strand, within Rru_A1308 at 1543.091 kb on - strand, within Rru_A1308
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 1,540,691 - Rru_A1305 0.77 +0.0 1,540,691 - Rru_A1305 0.77 +0.0 1,540,691 - Rru_A1305 0.77 +0.0 1,541,106 + Rru_A1306 0.39 +0.0 1,541,106 + Rru_A1306 0.39 -1.5 1,541,106 + Rru_A1306 0.39 +0.0 1,541,106 + Rru_A1306 0.39 +0.0 1,541,106 + Rru_A1306 0.39 +0.0 1,541,107 - Rru_A1306 0.40 +0.0 1,541,107 - Rru_A1306 0.40 +0.0 1,541,169 + Rru_A1306 0.47 -2.2 1,541,502 + Rru_A1306 0.87 +0.0 1,541,503 - Rru_A1306 0.87 +0.0 1,541,611 - +0.0 1,541,708 - Rru_A1307 0.20 +0.0 1,542,029 + Rru_A1307 0.82 -1.8 1,542,029 + Rru_A1307 0.82 +0.0 1,542,030 - Rru_A1307 0.82 -2.5 1,542,030 - Rru_A1307 0.82 +0.0 1,542,030 - Rru_A1307 0.82 +0.0 1,542,030 - Rru_A1307 0.82 +0.0 1,542,213 + +0.0 1,542,396 + +0.0 1,542,422 - Rru_A1308 0.11 +0.0 1,542,435 - Rru_A1308 0.12 -1.6 1,542,455 - Rru_A1308 0.13 -3.6 1,542,601 + Rru_A1308 0.21 -2.6 1,542,601 + Rru_A1308 0.21 +0.0 1,542,601 + Rru_A1308 0.21 +0.0 1,542,601 + Rru_A1308 0.21 +0.0 1,542,703 + Rru_A1308 0.27 -0.2 1,542,704 - Rru_A1308 0.27 -2.0 1,542,704 - Rru_A1308 0.27 +0.0 1,542,733 + Rru_A1308 0.28 -3.1 1,542,733 + Rru_A1308 0.28 -2.6 1,542,734 - Rru_A1308 0.28 +0.0 1,542,734 - Rru_A1308 0.28 +0.0 1,542,734 - Rru_A1308 0.28 +0.0 1,542,734 - Rru_A1308 0.28 -1.2 1,542,734 - Rru_A1308 0.28 -2.2 1,542,734 - Rru_A1308 0.28 +0.0 1,542,760 + Rru_A1308 0.30 -2.3 1,542,760 + Rru_A1308 0.30 -2.7 1,542,761 - Rru_A1308 0.30 -1.8 1,542,761 - Rru_A1308 0.30 -2.0 1,543,072 + Rru_A1308 0.47 -2.6 1,543,091 - Rru_A1308 0.48 -2.2
Or see this region's nucleotide sequence