Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5001 and Psyr_5002 overlap by 8 nucleotides Psyr_5002 and Psyr_5003 are separated by 244 nucleotides
Psyr_5001: Psyr_5001 - conserved hypothetical protein, at 5,930,376 to 5,932,823
_5001
Psyr_5002: Psyr_5002 - conserved hypothetical protein, at 5,932,816 to 5,933,298
_5002
Psyr_5003: Psyr_5003 - MscS Mechanosensitive ion channel, at 5,933,543 to 5,934,343
_5003
Position (kb) 5932
5933
5934 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5931.877 kb on - strand, within Psyr_5001 at 5931.974 kb on + strand, within Psyr_5001 at 5932.342 kb on + strand, within Psyr_5001 at 5932.801 kb on + strand at 5932.801 kb on + strand at 5932.801 kb on + strand at 5932.801 kb on + strand at 5932.801 kb on + strand at 5932.801 kb on + strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5932.802 kb on - strand at 5933.060 kb on + strand, within Psyr_5002 at 5933.060 kb on + strand, within Psyr_5002 at 5933.060 kb on + strand, within Psyr_5002 at 5933.060 kb on + strand, within Psyr_5002 at 5933.061 kb on - strand, within Psyr_5002 at 5933.061 kb on - strand, within Psyr_5002 at 5933.061 kb on - strand, within Psyr_5002 at 5933.061 kb on - strand, within Psyr_5002 at 5933.061 kb on - strand, within Psyr_5002 at 5933.069 kb on + strand, within Psyr_5002 at 5933.069 kb on + strand, within Psyr_5002 at 5933.069 kb on + strand, within Psyr_5002 at 5933.069 kb on + strand, within Psyr_5002 at 5933.069 kb on + strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.070 kb on - strand, within Psyr_5002 at 5933.174 kb on + strand, within Psyr_5002 at 5933.174 kb on + strand, within Psyr_5002 at 5933.249 kb on + strand, within Psyr_5002 at 5933.249 kb on + strand, within Psyr_5002 at 5933.250 kb on - strand at 5933.250 kb on - strand at 5933.298 kb on + strand at 5933.356 kb on + strand at 5933.356 kb on + strand at 5933.356 kb on + strand at 5933.356 kb on + strand at 5933.357 kb on - strand at 5933.424 kb on - strand at 5933.451 kb on - strand at 5933.451 kb on - strand at 5933.451 kb on - strand at 5933.451 kb on - strand at 5933.485 kb on + strand at 5933.485 kb on + strand at 5933.485 kb on + strand at 5933.485 kb on + strand at 5933.486 kb on - strand at 5933.523 kb on - strand at 5933.523 kb on - strand at 5933.748 kb on - strand, within Psyr_5003 at 5933.748 kb on - strand, within Psyr_5003 at 5933.748 kb on - strand, within Psyr_5003 at 5933.748 kb on - strand, within Psyr_5003 at 5933.748 kb on - strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.779 kb on + strand, within Psyr_5003 at 5933.780 kb on - strand, within Psyr_5003 at 5933.780 kb on - strand, within Psyr_5003 at 5933.780 kb on - strand, within Psyr_5003 at 5933.780 kb on - strand, within Psyr_5003 at 5933.780 kb on - strand, within Psyr_5003 at 5933.807 kb on + strand, within Psyr_5003 at 5933.807 kb on + strand, within Psyr_5003 at 5933.807 kb on + strand, within Psyr_5003 at 5933.807 kb on + strand, within Psyr_5003 at 5933.808 kb on - strand, within Psyr_5003 at 5933.808 kb on - strand, within Psyr_5003 at 5933.808 kb on - strand, within Psyr_5003 at 5933.808 kb on - strand, within Psyr_5003 at 5933.948 kb on - strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.189 kb on + strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.190 kb on - strand, within Psyr_5003 at 5934.264 kb on - strand at 5934.281 kb on + strand at 5934.281 kb on + strand at 5934.282 kb on - strand at 5934.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 5,931,877 - Psyr_5001 0.61 +0.5 5,931,974 + Psyr_5001 0.65 -0.7 5,932,342 + Psyr_5001 0.80 +1.8 5,932,801 + +0.4 5,932,801 + -1.7 5,932,801 + +0.8 5,932,801 + +0.1 5,932,801 + +0.8 5,932,801 + -0.5 5,932,802 - -1.4 5,932,802 - +1.1 5,932,802 - +0.3 5,932,802 - +0.5 5,932,802 - -0.9 5,932,802 - -1.9 5,932,802 - -1.7 5,933,060 + Psyr_5002 0.51 +1.8 5,933,060 + Psyr_5002 0.51 +1.5 5,933,060 + Psyr_5002 0.51 +1.4 5,933,060 + Psyr_5002 0.51 -0.2 5,933,061 - Psyr_5002 0.51 +0.3 5,933,061 - Psyr_5002 0.51 +0.1 5,933,061 - Psyr_5002 0.51 +0.2 5,933,061 - Psyr_5002 0.51 -0.4 5,933,061 - Psyr_5002 0.51 +0.8 5,933,069 + Psyr_5002 0.52 +0.1 5,933,069 + Psyr_5002 0.52 -0.4 5,933,069 + Psyr_5002 0.52 -0.5 5,933,069 + Psyr_5002 0.52 -1.0 5,933,069 + Psyr_5002 0.52 -0.3 5,933,070 - Psyr_5002 0.53 +1.5 5,933,070 - Psyr_5002 0.53 +0.7 5,933,070 - Psyr_5002 0.53 +0.2 5,933,070 - Psyr_5002 0.53 +0.1 5,933,070 - Psyr_5002 0.53 +0.1 5,933,070 - Psyr_5002 0.53 +2.7 5,933,174 + Psyr_5002 0.74 -0.2 5,933,174 + Psyr_5002 0.74 +0.3 5,933,249 + Psyr_5002 0.90 +0.1 5,933,249 + Psyr_5002 0.90 +0.5 5,933,250 - +0.6 5,933,250 - +2.4 5,933,298 + -1.0 5,933,356 + +0.4 5,933,356 + +0.9 5,933,356 + -1.4 5,933,356 + -0.1 5,933,357 - -0.5 5,933,424 - +1.1 5,933,451 - +0.8 5,933,451 - -1.2 5,933,451 - -0.1 5,933,451 - -0.2 5,933,485 + -0.0 5,933,485 + +2.6 5,933,485 + +0.8 5,933,485 + +0.1 5,933,486 - -0.7 5,933,523 - +1.1 5,933,523 - +0.9 5,933,748 - Psyr_5003 0.26 +0.1 5,933,748 - Psyr_5003 0.26 +0.2 5,933,748 - Psyr_5003 0.26 +0.8 5,933,748 - Psyr_5003 0.26 -0.2 5,933,748 - Psyr_5003 0.26 +0.2 5,933,779 + Psyr_5003 0.29 -0.1 5,933,779 + Psyr_5003 0.29 -0.9 5,933,779 + Psyr_5003 0.29 +0.6 5,933,779 + Psyr_5003 0.29 +0.5 5,933,779 + Psyr_5003 0.29 -1.0 5,933,779 + Psyr_5003 0.29 -0.7 5,933,779 + Psyr_5003 0.29 +0.3 5,933,779 + Psyr_5003 0.29 +0.7 5,933,780 - Psyr_5003 0.30 +0.4 5,933,780 - Psyr_5003 0.30 -1.2 5,933,780 - Psyr_5003 0.30 -1.2 5,933,780 - Psyr_5003 0.30 +1.7 5,933,780 - Psyr_5003 0.30 -0.6 5,933,807 + Psyr_5003 0.33 -0.4 5,933,807 + Psyr_5003 0.33 -0.2 5,933,807 + Psyr_5003 0.33 +1.4 5,933,807 + Psyr_5003 0.33 +0.6 5,933,808 - Psyr_5003 0.33 -3.2 5,933,808 - Psyr_5003 0.33 -0.1 5,933,808 - Psyr_5003 0.33 -0.6 5,933,808 - Psyr_5003 0.33 -0.1 5,933,948 - Psyr_5003 0.51 -0.0 5,934,189 + Psyr_5003 0.81 +0.8 5,934,189 + Psyr_5003 0.81 -0.2 5,934,189 + Psyr_5003 0.81 -0.2 5,934,189 + Psyr_5003 0.81 +0.5 5,934,189 + Psyr_5003 0.81 +0.2 5,934,189 + Psyr_5003 0.81 -0.2 5,934,190 - Psyr_5003 0.81 +0.3 5,934,190 - Psyr_5003 0.81 -0.4 5,934,190 - Psyr_5003 0.81 -0.4 5,934,190 - Psyr_5003 0.81 -0.4 5,934,190 - Psyr_5003 0.81 -0.3 5,934,190 - Psyr_5003 0.81 +0.8 5,934,190 - Psyr_5003 0.81 -0.6 5,934,264 - +1.2 5,934,281 + -0.4 5,934,281 + +0.5 5,934,282 - +0.6 5,934,282 - +0.1
Or see this region's nucleotide sequence