Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4573 and Psyr_4574 are separated by 121 nucleotides Psyr_4574 and Psyr_4575 are separated by 69 nucleotides Psyr_4575 and Psyr_4576 are separated by 242 nucleotides Psyr_4576 and Psyr_4577 are separated by 201 nucleotides
Psyr_4573: Psyr_4573 - Histidine triad (HIT) protein, at 5,436,921 to 5,437,259
_4573
Psyr_4574: Psyr_4574 - conserved hypothetical protein, at 5,437,381 to 5,438,028
_4574
Psyr_4575: Psyr_4575 - adenosylmethionine decarboxylase proenzyme, at 5,438,098 to 5,438,892
_4575
Psyr_4576: Psyr_4576 - OsmC-like protein, at 5,439,135 to 5,439,557
_4576
Psyr_4577: Psyr_4577 - Cyclic nucleotide-binding:Bacterial regulatory protein, Crp, at 5,439,759 to 5,440,403
_4577
Position (kb) 5438
5439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.106 kb on + strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.107 kb on - strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.222 kb on + strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5437.223 kb on - strand, within Psyr_4573 at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.036 kb on + strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.037 kb on - strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.038 kb on + strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.039 kb on - strand at 5438.049 kb on + strand at 5438.079 kb on + strand at 5438.102 kb on - strand at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.210 kb on + strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.211 kb on - strand, within Psyr_4575 at 5438.267 kb on + strand, within Psyr_4575 at 5438.318 kb on + strand, within Psyr_4575 at 5438.318 kb on + strand, within Psyr_4575 at 5438.318 kb on + strand, within Psyr_4575 at 5438.318 kb on + strand, within Psyr_4575 at 5438.318 kb on + strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.319 kb on - strand, within Psyr_4575 at 5438.380 kb on - strand, within Psyr_4575 at 5438.428 kb on - strand, within Psyr_4575 at 5438.428 kb on - strand, within Psyr_4575 at 5438.438 kb on + strand, within Psyr_4575 at 5438.439 kb on - strand, within Psyr_4575 at 5438.463 kb on + strand, within Psyr_4575 at 5438.463 kb on + strand, within Psyr_4575 at 5438.464 kb on - strand, within Psyr_4575 at 5438.464 kb on - strand, within Psyr_4575 at 5438.551 kb on - strand, within Psyr_4575 at 5438.679 kb on - strand, within Psyr_4575 at 5438.762 kb on + strand, within Psyr_4575 at 5438.762 kb on + strand, within Psyr_4575 at 5438.762 kb on + strand, within Psyr_4575 at 5438.762 kb on + strand, within Psyr_4575 at 5438.763 kb on - strand, within Psyr_4575 at 5438.763 kb on - strand, within Psyr_4575 at 5438.763 kb on - strand, within Psyr_4575 at 5438.777 kb on + strand, within Psyr_4575 at 5438.778 kb on - strand, within Psyr_4575 at 5438.778 kb on - strand, within Psyr_4575 at 5438.778 kb on - strand, within Psyr_4575 at 5438.778 kb on - strand, within Psyr_4575 at 5438.786 kb on + strand, within Psyr_4575 at 5438.786 kb on + strand, within Psyr_4575 at 5438.786 kb on + strand, within Psyr_4575 at 5438.786 kb on + strand, within Psyr_4575 at 5438.786 kb on + strand, within Psyr_4575 at 5438.787 kb on - strand, within Psyr_4575 at 5438.787 kb on - strand, within Psyr_4575 at 5438.817 kb on - strand at 5438.817 kb on - strand at 5438.828 kb on + strand at 5438.828 kb on + strand at 5438.829 kb on - strand at 5438.829 kb on - strand at 5438.829 kb on - strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.907 kb on + strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.908 kb on - strand at 5438.910 kb on - strand at 5438.971 kb on + strand at 5438.972 kb on - strand at 5438.972 kb on - strand at 5439.039 kb on + strand at 5439.039 kb on + strand at 5439.039 kb on + strand at 5439.039 kb on + strand at 5439.039 kb on + strand at 5439.040 kb on - strand at 5439.040 kb on - strand at 5439.040 kb on - strand at 5439.069 kb on + strand at 5439.070 kb on - strand at 5439.113 kb on - strand at 5439.113 kb on - strand at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.211 kb on + strand, within Psyr_4576 at 5439.212 kb on - strand, within Psyr_4576 at 5439.212 kb on - strand, within Psyr_4576 at 5439.212 kb on - strand, within Psyr_4576 at 5439.212 kb on - strand, within Psyr_4576 at 5439.230 kb on - strand, within Psyr_4576 at 5439.445 kb on - strand, within Psyr_4576 at 5439.445 kb on - strand, within Psyr_4576 at 5439.575 kb on - strand at 5439.575 kb on - strand at 5439.575 kb on - strand at 5439.594 kb on + strand at 5439.661 kb on + strand at 5439.661 kb on + strand at 5439.661 kb on + strand at 5439.661 kb on + strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.662 kb on - strand at 5439.669 kb on + strand at 5439.669 kb on + strand at 5439.670 kb on - strand at 5439.670 kb on - strand at 5439.670 kb on - strand at 5439.670 kb on - strand at 5439.711 kb on - strand at 5439.714 kb on + strand at 5439.714 kb on + strand at 5439.714 kb on + strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.715 kb on - strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.716 kb on + strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.717 kb on - strand at 5439.720 kb on + strand at 5439.720 kb on + strand at 5439.721 kb on - strand at 5439.736 kb on - strand at 5439.742 kb on + strand at 5439.742 kb on + strand at 5439.742 kb on + strand at 5439.743 kb on - strand at 5439.743 kb on - strand at 5439.769 kb on + strand at 5439.769 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 5,437,106 + Psyr_4573 0.55 +0.7 5,437,106 + Psyr_4573 0.55 +0.3 5,437,106 + Psyr_4573 0.55 +0.5 5,437,106 + Psyr_4573 0.55 -1.3 5,437,106 + Psyr_4573 0.55 +0.7 5,437,106 + Psyr_4573 0.55 +0.4 5,437,106 + Psyr_4573 0.55 +1.6 5,437,106 + Psyr_4573 0.55 -0.5 5,437,106 + Psyr_4573 0.55 -0.4 5,437,106 + Psyr_4573 0.55 +0.4 5,437,106 + Psyr_4573 0.55 +1.1 5,437,106 + Psyr_4573 0.55 +0.1 5,437,106 + Psyr_4573 0.55 -0.6 5,437,106 + Psyr_4573 0.55 +1.7 5,437,106 + Psyr_4573 0.55 -0.2 5,437,106 + Psyr_4573 0.55 -0.8 5,437,107 - Psyr_4573 0.55 +1.0 5,437,107 - Psyr_4573 0.55 +0.3 5,437,107 - Psyr_4573 0.55 -0.6 5,437,107 - Psyr_4573 0.55 +0.7 5,437,107 - Psyr_4573 0.55 -0.9 5,437,107 - Psyr_4573 0.55 -0.0 5,437,222 + Psyr_4573 0.89 +0.3 5,437,222 + Psyr_4573 0.89 +0.7 5,437,222 + Psyr_4573 0.89 +1.1 5,437,222 + Psyr_4573 0.89 -1.7 5,437,222 + Psyr_4573 0.89 +0.9 5,437,222 + Psyr_4573 0.89 +0.2 5,437,222 + Psyr_4573 0.89 +0.5 5,437,222 + Psyr_4573 0.89 -1.1 5,437,223 - Psyr_4573 0.89 +1.4 5,437,223 - Psyr_4573 0.89 -1.9 5,437,223 - Psyr_4573 0.89 +1.2 5,437,223 - Psyr_4573 0.89 +0.7 5,437,223 - Psyr_4573 0.89 +1.9 5,437,223 - Psyr_4573 0.89 +0.8 5,437,223 - Psyr_4573 0.89 +0.2 5,437,223 - Psyr_4573 0.89 +0.5 5,438,036 + -0.7 5,438,036 + -0.9 5,438,036 + -0.9 5,438,036 + +0.6 5,438,036 + -0.1 5,438,036 + +0.9 5,438,036 + -0.5 5,438,036 + +0.1 5,438,036 + -0.8 5,438,036 + +0.4 5,438,036 + +0.8 5,438,036 + +1.4 5,438,036 + +1.0 5,438,036 + +0.7 5,438,036 + +0.5 5,438,036 + -1.3 5,438,036 + -0.2 5,438,037 - -0.2 5,438,037 - +1.5 5,438,037 - +0.3 5,438,037 - -2.3 5,438,037 - +0.3 5,438,037 - +1.2 5,438,037 - -0.2 5,438,037 - +1.1 5,438,037 - +0.0 5,438,037 - +0.9 5,438,037 - +0.3 5,438,038 + -2.8 5,438,038 + +0.0 5,438,038 + +0.5 5,438,038 + -0.3 5,438,038 + +0.7 5,438,038 + -0.8 5,438,038 + -0.8 5,438,038 + -0.7 5,438,038 + +0.7 5,438,038 + -0.7 5,438,038 + -0.0 5,438,038 + +0.7 5,438,038 + -0.7 5,438,038 + +0.8 5,438,038 + +1.2 5,438,039 - +1.9 5,438,039 - +0.3 5,438,039 - -0.4 5,438,039 - +0.9 5,438,039 - +2.7 5,438,039 - +0.5 5,438,039 - -1.7 5,438,039 - +1.2 5,438,039 - -0.5 5,438,039 - +2.1 5,438,039 - +0.3 5,438,039 - +0.5 5,438,039 - +0.4 5,438,039 - -0.5 5,438,039 - +1.1 5,438,039 - +0.1 5,438,039 - +0.2 5,438,039 - +0.4 5,438,049 + -0.3 5,438,079 + +1.0 5,438,102 - +1.0 5,438,210 + Psyr_4575 0.14 +2.2 5,438,210 + Psyr_4575 0.14 -0.4 5,438,210 + Psyr_4575 0.14 -0.5 5,438,210 + Psyr_4575 0.14 +0.0 5,438,210 + Psyr_4575 0.14 +1.2 5,438,210 + Psyr_4575 0.14 -0.8 5,438,210 + Psyr_4575 0.14 +1.9 5,438,211 - Psyr_4575 0.14 +0.3 5,438,211 - Psyr_4575 0.14 +0.1 5,438,211 - Psyr_4575 0.14 -1.5 5,438,211 - Psyr_4575 0.14 +0.7 5,438,211 - Psyr_4575 0.14 +0.2 5,438,211 - Psyr_4575 0.14 +0.5 5,438,211 - Psyr_4575 0.14 -0.1 5,438,211 - Psyr_4575 0.14 -0.6 5,438,211 - Psyr_4575 0.14 -0.1 5,438,267 + Psyr_4575 0.21 +2.7 5,438,318 + Psyr_4575 0.28 -0.3 5,438,318 + Psyr_4575 0.28 +1.1 5,438,318 + Psyr_4575 0.28 +0.8 5,438,318 + Psyr_4575 0.28 +0.1 5,438,318 + Psyr_4575 0.28 -0.3 5,438,319 - Psyr_4575 0.28 -0.8 5,438,319 - Psyr_4575 0.28 -0.3 5,438,319 - Psyr_4575 0.28 +0.8 5,438,319 - Psyr_4575 0.28 -1.1 5,438,319 - Psyr_4575 0.28 -0.1 5,438,319 - Psyr_4575 0.28 +3.0 5,438,319 - Psyr_4575 0.28 +0.5 5,438,380 - Psyr_4575 0.35 +0.4 5,438,428 - Psyr_4575 0.42 +1.7 5,438,428 - Psyr_4575 0.42 +0.0 5,438,438 + Psyr_4575 0.43 +0.4 5,438,439 - Psyr_4575 0.43 +0.3 5,438,463 + Psyr_4575 0.46 +0.3 5,438,463 + Psyr_4575 0.46 -0.5 5,438,464 - Psyr_4575 0.46 +1.2 5,438,464 - Psyr_4575 0.46 -1.8 5,438,551 - Psyr_4575 0.57 +0.7 5,438,679 - Psyr_4575 0.73 +0.2 5,438,762 + Psyr_4575 0.84 +0.5 5,438,762 + Psyr_4575 0.84 +0.2 5,438,762 + Psyr_4575 0.84 -0.6 5,438,762 + Psyr_4575 0.84 +0.1 5,438,763 - Psyr_4575 0.84 +0.5 5,438,763 - Psyr_4575 0.84 +3.2 5,438,763 - Psyr_4575 0.84 +0.7 5,438,777 + Psyr_4575 0.85 +0.4 5,438,778 - Psyr_4575 0.86 +1.0 5,438,778 - Psyr_4575 0.86 +0.9 5,438,778 - Psyr_4575 0.86 +1.1 5,438,778 - Psyr_4575 0.86 +0.7 5,438,786 + Psyr_4575 0.87 +0.4 5,438,786 + Psyr_4575 0.87 -0.7 5,438,786 + Psyr_4575 0.87 +1.5 5,438,786 + Psyr_4575 0.87 -0.2 5,438,786 + Psyr_4575 0.87 -1.3 5,438,787 - Psyr_4575 0.87 -0.2 5,438,787 - Psyr_4575 0.87 +0.4 5,438,817 - +0.9 5,438,817 - +2.1 5,438,828 + +0.8 5,438,828 + +0.3 5,438,829 - +0.2 5,438,829 - -0.1 5,438,829 - +0.2 5,438,907 + -0.1 5,438,907 + +2.2 5,438,907 + -0.2 5,438,907 + +0.2 5,438,907 + +0.4 5,438,907 + +0.4 5,438,907 + -1.7 5,438,907 + +0.1 5,438,907 + -0.9 5,438,907 + -1.3 5,438,907 + +0.3 5,438,908 - +0.2 5,438,908 - -0.8 5,438,908 - -0.1 5,438,908 - +0.5 5,438,908 - +0.9 5,438,908 - -0.1 5,438,908 - +0.7 5,438,908 - +1.3 5,438,908 - +0.8 5,438,908 - -1.0 5,438,908 - -1.3 5,438,910 - +0.9 5,438,971 + +0.4 5,438,972 - -0.8 5,438,972 - -0.0 5,439,039 + -1.2 5,439,039 + +0.1 5,439,039 + -0.2 5,439,039 + -0.1 5,439,039 + +0.8 5,439,040 - -1.3 5,439,040 - +0.4 5,439,040 - +0.2 5,439,069 + +1.0 5,439,070 - +0.4 5,439,113 - -0.7 5,439,113 - +0.7 5,439,211 + Psyr_4576 0.18 -2.3 5,439,211 + Psyr_4576 0.18 +0.7 5,439,211 + Psyr_4576 0.18 +1.1 5,439,211 + Psyr_4576 0.18 +1.2 5,439,211 + Psyr_4576 0.18 +0.1 5,439,211 + Psyr_4576 0.18 +1.2 5,439,211 + Psyr_4576 0.18 +0.8 5,439,212 - Psyr_4576 0.18 -0.5 5,439,212 - Psyr_4576 0.18 +0.2 5,439,212 - Psyr_4576 0.18 +2.7 5,439,212 - Psyr_4576 0.18 -1.1 5,439,230 - Psyr_4576 0.22 +0.5 5,439,445 - Psyr_4576 0.73 +0.3 5,439,445 - Psyr_4576 0.73 +0.1 5,439,575 - -1.8 5,439,575 - +1.5 5,439,575 - +0.1 5,439,594 + +1.7 5,439,661 + +0.9 5,439,661 + +0.1 5,439,661 + +0.2 5,439,661 + +0.0 5,439,662 - +1.0 5,439,662 - +1.4 5,439,662 - +4.3 5,439,662 - +0.5 5,439,662 - +2.0 5,439,662 - +1.9 5,439,662 - -1.3 5,439,662 - +1.2 5,439,669 + -0.1 5,439,669 + +0.8 5,439,670 - +1.4 5,439,670 - +1.6 5,439,670 - +0.3 5,439,670 - +0.5 5,439,711 - +0.1 5,439,714 + +1.5 5,439,714 + -1.3 5,439,714 + -1.7 5,439,715 - -0.1 5,439,715 - +0.4 5,439,715 - -0.7 5,439,715 - -0.5 5,439,715 - +1.4 5,439,715 - +2.8 5,439,715 - +0.2 5,439,715 - -1.1 5,439,715 - +0.7 5,439,715 - +0.9 5,439,716 + +0.2 5,439,716 + +0.1 5,439,716 + +0.9 5,439,716 + +1.1 5,439,716 + +0.1 5,439,716 + -0.3 5,439,716 + +1.1 5,439,717 - +0.8 5,439,717 - +1.2 5,439,717 - -0.4 5,439,717 - -0.3 5,439,717 - -0.7 5,439,717 - +0.6 5,439,717 - +1.4 5,439,717 - +1.2 5,439,717 - +1.0 5,439,717 - +0.3 5,439,717 - +2.5 5,439,720 + +0.1 5,439,720 + +1.3 5,439,721 - +1.4 5,439,736 - +1.5 5,439,742 + +1.3 5,439,742 + -0.2 5,439,742 + +1.3 5,439,743 - +0.4 5,439,743 - +0.8 5,439,769 + +0.4 5,439,769 + +1.3
Or see this region's nucleotide sequence