Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4147 and Psyr_4148 are separated by 75 nucleotides Psyr_4148 and Psyr_4149 are separated by 10 nucleotides Psyr_4149 and Psyr_4150 are separated by 2 nucleotides
Psyr_4147: Psyr_4147 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 4,934,535 to 4,936,070
_4147
Psyr_4148: Psyr_4148 - sigma 54 modulation protein / SSU ribosomal protein S30P, at 4,936,146 to 4,936,454
_4148
Psyr_4149: Psyr_4149 - PTS IIA-like nitrogen-regulatory protein PtsN, at 4,936,465 to 4,936,929
_4149
Psyr_4150: Psyr_4150 - Uncharacterized P-loop ATPase protein UPF0042, at 4,936,932 to 4,937,789
_4150
Position (kb) 4936
4937 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4936.120 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.200 kb on + strand, within Psyr_4148 at 4936.247 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.409 kb on + strand, within Psyr_4148 at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.976 kb on + strand at 4937.010 kb on + strand at 4937.107 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.165 kb on - strand, within Psyr_4150 at 4937.177 kb on + strand, within Psyr_4150 at 4937.197 kb on - strand, within Psyr_4150 at 4937.294 kb on - strand, within Psyr_4150 at 4937.294 kb on - strand, within Psyr_4150 at 4937.371 kb on - strand, within Psyr_4150 at 4937.401 kb on + strand, within Psyr_4150
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 4,936,120 + -2.0 4,936,145 + -0.3 4,936,145 + -0.2 4,936,145 + +1.0 4,936,145 + -0.4 4,936,200 + Psyr_4148 0.17 +0.5 4,936,247 + Psyr_4148 0.33 -0.5 4,936,356 + Psyr_4148 0.68 +2.0 4,936,356 + Psyr_4148 0.68 +0.7 4,936,356 + Psyr_4148 0.68 +1.2 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 -0.7 4,936,356 + Psyr_4148 0.68 +0.4 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 -1.0 4,936,356 + Psyr_4148 0.68 -1.7 4,936,356 + Psyr_4148 0.68 -1.3 4,936,356 + Psyr_4148 0.68 +1.1 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 +1.1 4,936,356 + Psyr_4148 0.68 -0.8 4,936,356 + Psyr_4148 0.68 -0.5 4,936,356 + Psyr_4148 0.68 +0.9 4,936,356 + Psyr_4148 0.68 -0.2 4,936,356 + Psyr_4148 0.68 +0.8 4,936,356 + Psyr_4148 0.68 +0.7 4,936,356 + Psyr_4148 0.68 +0.9 4,936,356 + Psyr_4148 0.68 -0.4 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 +0.9 4,936,356 + Psyr_4148 0.68 +1.0 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 +1.5 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 +1.8 4,936,356 + Psyr_4148 0.68 +3.0 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +0.7 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -1.5 4,936,357 - Psyr_4148 0.68 -2.3 4,936,357 - Psyr_4148 0.68 -0.0 4,936,357 - Psyr_4148 0.68 +1.1 4,936,357 - Psyr_4148 0.68 -2.7 4,936,357 - Psyr_4148 0.68 -0.9 4,936,387 + Psyr_4148 0.78 +0.5 4,936,387 + Psyr_4148 0.78 +1.2 4,936,387 + Psyr_4148 0.78 -0.1 4,936,409 + Psyr_4148 0.85 +1.3 4,936,463 + +0.7 4,936,463 + +0.1 4,936,463 + -0.5 4,936,463 + +0.2 4,936,976 + -0.3 4,937,010 + -0.0 4,937,107 + Psyr_4150 0.20 -0.3 4,937,164 + Psyr_4150 0.27 -0.0 4,937,164 + Psyr_4150 0.27 -0.8 4,937,164 + Psyr_4150 0.27 +0.3 4,937,165 - Psyr_4150 0.27 +0.6 4,937,177 + Psyr_4150 0.29 -0.2 4,937,197 - Psyr_4150 0.31 -2.5 4,937,294 - Psyr_4150 0.42 +0.7 4,937,294 - Psyr_4150 0.42 -0.2 4,937,371 - Psyr_4150 0.51 -0.9 4,937,401 + Psyr_4150 0.55 +0.0
Or see this region's nucleotide sequence