Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3524 and Psyr_3525 are separated by 3 nucleotides Psyr_3525 and Psyr_3526 are separated by 161 nucleotides
Psyr_3524: Psyr_3524 - Aldehyde dehydrogenase (NAD+), at 4,200,283 to 4,201,800
_3524
Psyr_3525: Psyr_3525 - 3-hydroxyisobutyrate dehydrogenase, at 4,201,804 to 4,202,694
_3525
Psyr_3526: Psyr_3526 - transcriptional regulator, LysR family, at 4,202,856 to 4,203,761
_3526
Position (kb) 4201
4202
4203 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4201.321 kb on - strand, within Psyr_3524 at 4201.349 kb on + strand, within Psyr_3524 at 4201.349 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.358 kb on + strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.359 kb on - strand, within Psyr_3524 at 4201.421 kb on + strand, within Psyr_3524 at 4201.421 kb on + strand, within Psyr_3524 at 4201.422 kb on - strand, within Psyr_3524 at 4201.422 kb on - strand, within Psyr_3524 at 4201.422 kb on - strand, within Psyr_3524 at 4201.424 kb on + strand, within Psyr_3524 at 4201.425 kb on - strand, within Psyr_3524 at 4201.425 kb on - strand, within Psyr_3524 at 4201.547 kb on + strand, within Psyr_3524 at 4201.547 kb on + strand, within Psyr_3524 at 4201.548 kb on - strand, within Psyr_3524 at 4201.548 kb on - strand, within Psyr_3524 at 4201.548 kb on - strand, within Psyr_3524 at 4201.548 kb on - strand, within Psyr_3524 at 4201.548 kb on - strand, within Psyr_3524 at 4201.733 kb on + strand at 4201.733 kb on + strand at 4201.733 kb on + strand at 4201.733 kb on + strand at 4201.733 kb on + strand at 4201.734 kb on - strand at 4201.734 kb on - strand at 4201.734 kb on - strand at 4201.734 kb on - strand at 4201.734 kb on - strand at 4201.805 kb on + strand at 4201.806 kb on - strand at 4202.006 kb on + strand, within Psyr_3525 at 4202.006 kb on + strand, within Psyr_3525 at 4202.007 kb on - strand, within Psyr_3525 at 4202.007 kb on - strand, within Psyr_3525 at 4202.424 kb on + strand, within Psyr_3525 at 4202.727 kb on + strand at 4202.825 kb on + strand at 4202.825 kb on + strand at 4202.825 kb on + strand at 4202.825 kb on + strand at 4203.063 kb on + strand, within Psyr_3526 at 4203.063 kb on + strand, within Psyr_3526 at 4203.063 kb on + strand, within Psyr_3526 at 4203.063 kb on + strand, within Psyr_3526 at 4203.063 kb on + strand, within Psyr_3526 at 4203.210 kb on + strand, within Psyr_3526 at 4203.211 kb on - strand, within Psyr_3526 at 4203.211 kb on - strand, within Psyr_3526 at 4203.407 kb on - strand, within Psyr_3526 at 4203.407 kb on - strand, within Psyr_3526 at 4203.607 kb on + strand, within Psyr_3526 at 4203.607 kb on + strand, within Psyr_3526 at 4203.607 kb on + strand, within Psyr_3526 at 4203.608 kb on - strand, within Psyr_3526
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 4,201,321 - Psyr_3524 0.68 +0.1 4,201,349 + Psyr_3524 0.70 +0.7 4,201,349 + Psyr_3524 0.70 -1.1 4,201,358 + Psyr_3524 0.71 -2.9 4,201,358 + Psyr_3524 0.71 +0.4 4,201,358 + Psyr_3524 0.71 -0.8 4,201,358 + Psyr_3524 0.71 -0.7 4,201,358 + Psyr_3524 0.71 -0.4 4,201,358 + Psyr_3524 0.71 -0.2 4,201,358 + Psyr_3524 0.71 +0.6 4,201,358 + Psyr_3524 0.71 -0.5 4,201,359 - Psyr_3524 0.71 +0.9 4,201,359 - Psyr_3524 0.71 -2.9 4,201,359 - Psyr_3524 0.71 +1.3 4,201,359 - Psyr_3524 0.71 -1.1 4,201,359 - Psyr_3524 0.71 -0.6 4,201,359 - Psyr_3524 0.71 +0.0 4,201,359 - Psyr_3524 0.71 -0.1 4,201,359 - Psyr_3524 0.71 -0.3 4,201,359 - Psyr_3524 0.71 +0.8 4,201,359 - Psyr_3524 0.71 -0.0 4,201,359 - Psyr_3524 0.71 -0.9 4,201,359 - Psyr_3524 0.71 -1.6 4,201,359 - Psyr_3524 0.71 -1.5 4,201,359 - Psyr_3524 0.71 -0.1 4,201,421 + Psyr_3524 0.75 -3.6 4,201,421 + Psyr_3524 0.75 +0.3 4,201,422 - Psyr_3524 0.75 -0.8 4,201,422 - Psyr_3524 0.75 +0.4 4,201,422 - Psyr_3524 0.75 -0.0 4,201,424 + Psyr_3524 0.75 +1.3 4,201,425 - Psyr_3524 0.75 -1.3 4,201,425 - Psyr_3524 0.75 +0.7 4,201,547 + Psyr_3524 0.83 -0.9 4,201,547 + Psyr_3524 0.83 -1.1 4,201,548 - Psyr_3524 0.83 +0.3 4,201,548 - Psyr_3524 0.83 +1.5 4,201,548 - Psyr_3524 0.83 +0.8 4,201,548 - Psyr_3524 0.83 +0.0 4,201,548 - Psyr_3524 0.83 -0.6 4,201,733 + +0.1 4,201,733 + +1.7 4,201,733 + +0.3 4,201,733 + -0.1 4,201,733 + -0.0 4,201,734 - +0.4 4,201,734 - +0.4 4,201,734 - -0.0 4,201,734 - -0.5 4,201,734 - -0.1 4,201,805 + +0.5 4,201,806 - -0.2 4,202,006 + Psyr_3525 0.23 +0.1 4,202,006 + Psyr_3525 0.23 +0.2 4,202,007 - Psyr_3525 0.23 +0.6 4,202,007 - Psyr_3525 0.23 -0.1 4,202,424 + Psyr_3525 0.70 +1.8 4,202,727 + -0.9 4,202,825 + +0.2 4,202,825 + +0.8 4,202,825 + +1.0 4,202,825 + -1.2 4,203,063 + Psyr_3526 0.23 +0.2 4,203,063 + Psyr_3526 0.23 +0.4 4,203,063 + Psyr_3526 0.23 +0.5 4,203,063 + Psyr_3526 0.23 -0.5 4,203,063 + Psyr_3526 0.23 -0.5 4,203,210 + Psyr_3526 0.39 +0.9 4,203,211 - Psyr_3526 0.39 -0.7 4,203,211 - Psyr_3526 0.39 +0.6 4,203,407 - Psyr_3526 0.61 -2.3 4,203,407 - Psyr_3526 0.61 -0.4 4,203,607 + Psyr_3526 0.83 +0.3 4,203,607 + Psyr_3526 0.83 -1.5 4,203,607 + Psyr_3526 0.83 -0.5 4,203,608 - Psyr_3526 0.83 +0.6
Or see this region's nucleotide sequence