Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3513 and Psyr_3514 are separated by 42 nucleotides Psyr_3514 and Psyr_3515 are separated by 255 nucleotides
Psyr_3513: Psyr_3513 - FAD dependent oxidoreductase, at 4,187,968 to 4,189,254
_3513
Psyr_3514: Psyr_3514 - delta-1-piperideine-6-carboxylate dehydrogenase, at 4,189,297 to 4,190,787
_3514
Psyr_3515: Psyr_3515 - 4-aminobutyrate aminotransferase apoenzyme, at 4,191,043 to 4,192,290
_3515
Position (kb) 4189
4190
4191 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4188.332 kb on + strand, within Psyr_3513 at 4188.411 kb on + strand, within Psyr_3513 at 4188.485 kb on + strand, within Psyr_3513 at 4188.488 kb on + strand, within Psyr_3513 at 4188.488 kb on + strand, within Psyr_3513 at 4188.488 kb on + strand, within Psyr_3513 at 4188.488 kb on + strand, within Psyr_3513 at 4188.488 kb on + strand, within Psyr_3513 at 4188.489 kb on - strand, within Psyr_3513 at 4188.489 kb on - strand, within Psyr_3513 at 4188.489 kb on - strand, within Psyr_3513 at 4188.489 kb on - strand, within Psyr_3513 at 4188.489 kb on - strand, within Psyr_3513 at 4188.491 kb on + strand, within Psyr_3513 at 4188.539 kb on + strand, within Psyr_3513 at 4188.540 kb on - strand, within Psyr_3513 at 4188.574 kb on + strand, within Psyr_3513 at 4188.574 kb on + strand, within Psyr_3513 at 4188.575 kb on - strand, within Psyr_3513 at 4188.575 kb on - strand, within Psyr_3513 at 4188.575 kb on - strand, within Psyr_3513 at 4188.575 kb on - strand, within Psyr_3513 at 4188.695 kb on + strand, within Psyr_3513 at 4188.695 kb on + strand, within Psyr_3513 at 4188.695 kb on + strand, within Psyr_3513 at 4188.696 kb on - strand, within Psyr_3513 at 4188.696 kb on - strand, within Psyr_3513 at 4188.696 kb on - strand, within Psyr_3513 at 4188.992 kb on - strand, within Psyr_3513 at 4188.992 kb on - strand, within Psyr_3513 at 4189.278 kb on - strand at 4189.280 kb on + strand at 4189.280 kb on + strand at 4189.280 kb on + strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.281 kb on - strand at 4189.340 kb on + strand at 4189.340 kb on + strand at 4189.340 kb on + strand at 4189.341 kb on - strand at 4189.370 kb on + strand at 4189.370 kb on + strand at 4189.370 kb on + strand at 4189.370 kb on + strand at 4189.370 kb on + strand at 4189.371 kb on - strand at 4189.371 kb on - strand at 4189.371 kb on - strand at 4189.371 kb on - strand at 4189.564 kb on + strand, within Psyr_3514 at 4189.616 kb on + strand, within Psyr_3514 at 4189.616 kb on + strand, within Psyr_3514 at 4189.691 kb on + strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.692 kb on - strand, within Psyr_3514 at 4189.706 kb on + strand, within Psyr_3514 at 4189.707 kb on - strand, within Psyr_3514 at 4190.429 kb on + strand, within Psyr_3514 at 4190.430 kb on - strand, within Psyr_3514 at 4190.430 kb on - strand, within Psyr_3514 at 4190.430 kb on - strand, within Psyr_3514 at 4190.551 kb on - strand, within Psyr_3514 at 4190.551 kb on - strand, within Psyr_3514 at 4190.551 kb on - strand, within Psyr_3514 at 4190.551 kb on - strand, within Psyr_3514 at 4190.563 kb on + strand, within Psyr_3514 at 4190.563 kb on + strand, within Psyr_3514 at 4190.563 kb on + strand, within Psyr_3514 at 4190.564 kb on - strand, within Psyr_3514 at 4190.564 kb on - strand, within Psyr_3514 at 4190.564 kb on - strand, within Psyr_3514 at 4190.657 kb on - strand at 4190.718 kb on - strand at 4190.770 kb on + strand at 4190.770 kb on + strand at 4190.773 kb on + strand at 4190.773 kb on + strand at 4190.773 kb on + strand at 4190.838 kb on - strand at 4190.860 kb on + strand at 4190.915 kb on + strand at 4190.915 kb on + strand at 4190.915 kb on + strand at 4190.935 kb on - strand at 4191.139 kb on + strand at 4191.140 kb on - strand at 4191.140 kb on - strand at 4191.140 kb on - strand at 4191.268 kb on + strand, within Psyr_3515 at 4191.268 kb on + strand, within Psyr_3515 at 4191.269 kb on - strand, within Psyr_3515 at 4191.269 kb on - strand, within Psyr_3515 at 4191.476 kb on - strand, within Psyr_3515 at 4191.478 kb on - strand, within Psyr_3515
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 4,188,332 + Psyr_3513 0.28 -0.4 4,188,411 + Psyr_3513 0.34 -0.4 4,188,485 + Psyr_3513 0.40 +0.6 4,188,488 + Psyr_3513 0.40 +1.8 4,188,488 + Psyr_3513 0.40 +0.6 4,188,488 + Psyr_3513 0.40 -0.1 4,188,488 + Psyr_3513 0.40 +0.6 4,188,488 + Psyr_3513 0.40 +0.3 4,188,489 - Psyr_3513 0.40 +0.4 4,188,489 - Psyr_3513 0.40 +1.0 4,188,489 - Psyr_3513 0.40 +0.0 4,188,489 - Psyr_3513 0.40 +1.0 4,188,489 - Psyr_3513 0.40 -0.5 4,188,491 + Psyr_3513 0.41 +0.2 4,188,539 + Psyr_3513 0.44 +2.5 4,188,540 - Psyr_3513 0.44 +0.8 4,188,574 + Psyr_3513 0.47 -0.1 4,188,574 + Psyr_3513 0.47 +0.2 4,188,575 - Psyr_3513 0.47 -0.1 4,188,575 - Psyr_3513 0.47 +3.9 4,188,575 - Psyr_3513 0.47 +0.3 4,188,575 - Psyr_3513 0.47 -0.5 4,188,695 + Psyr_3513 0.56 -0.1 4,188,695 + Psyr_3513 0.56 -0.4 4,188,695 + Psyr_3513 0.56 +0.7 4,188,696 - Psyr_3513 0.57 -2.9 4,188,696 - Psyr_3513 0.57 +0.2 4,188,696 - Psyr_3513 0.57 -0.5 4,188,992 - Psyr_3513 0.80 +0.6 4,188,992 - Psyr_3513 0.80 +0.7 4,189,278 - +1.7 4,189,280 + -1.9 4,189,280 + -0.1 4,189,280 + +0.3 4,189,281 - -0.3 4,189,281 - +1.0 4,189,281 - +0.4 4,189,281 - +0.9 4,189,281 - -0.1 4,189,281 - -0.7 4,189,281 - +0.3 4,189,281 - -0.1 4,189,281 - +1.6 4,189,281 - +0.5 4,189,340 + -0.1 4,189,340 + +1.2 4,189,340 + -0.7 4,189,341 - +0.5 4,189,370 + +0.5 4,189,370 + -0.6 4,189,370 + -1.0 4,189,370 + +0.3 4,189,370 + +0.1 4,189,371 - +0.5 4,189,371 - -0.9 4,189,371 - -0.0 4,189,371 - +0.1 4,189,564 + Psyr_3514 0.18 -0.1 4,189,616 + Psyr_3514 0.21 -0.3 4,189,616 + Psyr_3514 0.21 -2.0 4,189,691 + Psyr_3514 0.26 -1.0 4,189,692 - Psyr_3514 0.26 -0.5 4,189,692 - Psyr_3514 0.26 +0.6 4,189,692 - Psyr_3514 0.26 -0.2 4,189,692 - Psyr_3514 0.26 -1.9 4,189,692 - Psyr_3514 0.26 -0.9 4,189,692 - Psyr_3514 0.26 -0.5 4,189,692 - Psyr_3514 0.26 +0.1 4,189,706 + Psyr_3514 0.27 -0.3 4,189,707 - Psyr_3514 0.27 +0.2 4,190,429 + Psyr_3514 0.76 -0.8 4,190,430 - Psyr_3514 0.76 -0.7 4,190,430 - Psyr_3514 0.76 -0.7 4,190,430 - Psyr_3514 0.76 +0.7 4,190,551 - Psyr_3514 0.84 -0.4 4,190,551 - Psyr_3514 0.84 +1.1 4,190,551 - Psyr_3514 0.84 +0.1 4,190,551 - Psyr_3514 0.84 +0.5 4,190,563 + Psyr_3514 0.85 -0.5 4,190,563 + Psyr_3514 0.85 +0.1 4,190,563 + Psyr_3514 0.85 +0.7 4,190,564 - Psyr_3514 0.85 +0.6 4,190,564 - Psyr_3514 0.85 -0.2 4,190,564 - Psyr_3514 0.85 -0.9 4,190,657 - +0.8 4,190,718 - -1.5 4,190,770 + +0.2 4,190,770 + -1.1 4,190,773 + +2.5 4,190,773 + +0.0 4,190,773 + -0.2 4,190,838 - -0.4 4,190,860 + -0.0 4,190,915 + +0.3 4,190,915 + +0.5 4,190,915 + -0.1 4,190,935 - -0.1 4,191,139 + +1.9 4,191,140 - +1.0 4,191,140 - +0.3 4,191,140 - -0.1 4,191,268 + Psyr_3515 0.18 +2.9 4,191,268 + Psyr_3515 0.18 -0.1 4,191,269 - Psyr_3515 0.18 -0.1 4,191,269 - Psyr_3515 0.18 +0.3 4,191,476 - Psyr_3515 0.35 -0.3 4,191,478 - Psyr_3515 0.35 +0.7
Or see this region's nucleotide sequence