Experiment: KB with Rotenone 0.25 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Psyr_3423 and Psyr_3424 are separated by 14 nucleotides Psyr_3424 and Psyr_3425 are separated by 152 nucleotides Psyr_3425 and Psyr_3426 are separated by 61 nucleotides 
        Psyr_3423: Psyr_3423 - coproporphyrinogen III oxidase, anaerobic, at 4,087,450 to 4,088,835 
        _3423 
         
        
        Psyr_3424: Psyr_3424 - conserved hypothetical protein, at 4,088,850 to 4,089,317 
        _3424 
         
        
        Psyr_3425: Psyr_3425 - Cyclic nucleotide-binding:Bacterial regulatory protein, Crp, at 4,089,470 to 4,090,204 
        _3425 
         
        
        Psyr_3426: Psyr_3426 - adenine phosphoribosyltransferase, at 4,090,266 to 4,090,814 
        _3426 
         Position (kb) 4089 
4090 
4091 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 4088.656 kb on + strand, within Psyr_3423 at 4088.657 kb on - strand, within Psyr_3423 at 4088.657 kb on - strand, within Psyr_3423 at 4088.657 kb on - strand, within Psyr_3423 at 4088.789 kb on - strand at 4088.831 kb on + strand at 4088.831 kb on + strand at 4088.851 kb on + strand at 4088.852 kb on - strand at 4088.966 kb on + strand, within Psyr_3424 at 4088.967 kb on - strand, within Psyr_3424 at 4088.967 kb on - strand, within Psyr_3424 at 4088.967 kb on - strand, within Psyr_3424 at 4088.967 kb on - strand, within Psyr_3424 at 4089.253 kb on + strand, within Psyr_3424 at 4089.253 kb on + strand, within Psyr_3424 at 4089.253 kb on + strand, within Psyr_3424 at 4089.253 kb on + strand, within Psyr_3424 at 4089.254 kb on - strand, within Psyr_3424 at 4089.254 kb on - strand, within Psyr_3424 at 4089.254 kb on - strand, within Psyr_3424 at 4089.254 kb on - strand, within Psyr_3424 at 4089.254 kb on - strand, within Psyr_3424 at 4089.348 kb on - strand at 4089.440 kb on + strand at 4089.440 kb on + strand at 4089.440 kb on + strand at 4089.440 kb on + strand at 4089.440 kb on + strand at 4089.440 kb on + strand at 4089.441 kb on - strand at 4089.441 kb on - strand at 4089.441 kb on - strand at 4089.464 kb on + strand at 4089.464 kb on + strand at 4089.555 kb on + strand, within Psyr_3425 at 4089.555 kb on + strand, within Psyr_3425 at 4089.660 kb on - strand, within Psyr_3425 at 4089.694 kb on + strand, within Psyr_3425 at 4089.751 kb on - strand at 4090.095 kb on + strand, within Psyr_3425 at 4090.096 kb on - strand, within Psyr_3425 at 4090.099 kb on + strand, within Psyr_3425 at 4090.146 kb on + strand at 4090.146 kb on + strand at 4090.146 kb on + strand at 4090.146 kb on + strand at 4090.146 kb on + strand at 4090.147 kb on - strand at 4090.147 kb on - strand at 4090.147 kb on - strand at 4090.147 kb on - strand at 4090.231 kb on + strand at 4090.232 kb on - strand at 4090.410 kb on + strand, within Psyr_3426 at 4090.410 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.540 kb on + strand, within Psyr_3426 at 4090.541 kb on - strand, within Psyr_3426 at 4090.541 kb on - strand, within Psyr_3426 at 4090.541 kb on - strand, within Psyr_3426 at 4090.541 kb on - strand, within Psyr_3426 at 4090.541 kb on - strand, within Psyr_3426 at 4090.554 kb on + strand, within Psyr_3426 at 4090.567 kb on - strand, within Psyr_3426 at 4090.578 kb on + strand, within Psyr_3426 at 4090.578 kb on + strand, within Psyr_3426 at 4090.578 kb on + strand, within Psyr_3426 at 4090.705 kb on - strand, within Psyr_3426 at 4090.869 kb on + strand at 4090.870 kb on - strand at 4090.918 kb on - strand at 4090.918 kb on - strand at 4090.918 kb on - strand at 4091.097 kb on + strand at 4091.097 kb on + strand at 4091.098 kb on - strand at 4091.142 kb on + strand at 4091.142 kb on + strand at 4091.142 kb on + strand at 4091.143 kb on - strand at 4091.143 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction KB with Rotenone 0.25 mg/ml remove 4,088,656 +  Psyr_3423 0.87  +0.2 4,088,657 -  Psyr_3423 0.87  -1.2 4,088,657 -  Psyr_3423 0.87  -0.3 4,088,657 -  Psyr_3423 0.87  +1.0 4,088,789 -  +0.1 4,088,831 +  -0.8 4,088,831 +  -0.2 4,088,851 +  +1.1 4,088,852 -  -0.6 4,088,966 +  Psyr_3424 0.25  +0.1 4,088,967 -  Psyr_3424 0.25  -0.1 4,088,967 -  Psyr_3424 0.25  +0.1 4,088,967 -  Psyr_3424 0.25  -0.0 4,088,967 -  Psyr_3424 0.25  +0.4 4,089,253 +  Psyr_3424 0.86  +1.4 4,089,253 +  Psyr_3424 0.86  -1.0 4,089,253 +  Psyr_3424 0.86  +0.1 4,089,253 +  Psyr_3424 0.86  +1.0 4,089,254 -  Psyr_3424 0.86  +0.2 4,089,254 -  Psyr_3424 0.86  +0.6 4,089,254 -  Psyr_3424 0.86  -1.4 4,089,254 -  Psyr_3424 0.86  -0.5 4,089,254 -  Psyr_3424 0.86  +0.4 4,089,348 -  -0.9 4,089,440 +  -1.4 4,089,440 +  +1.5 4,089,440 +  -2.2 4,089,440 +  +0.2 4,089,440 +  -0.2 4,089,440 +  -0.7 4,089,441 -  -2.7 4,089,441 -  +0.4 4,089,441 -  +1.4 4,089,464 +  +2.6 4,089,464 +  +0.0 4,089,555 +  Psyr_3425 0.12  +1.1 4,089,555 +  Psyr_3425 0.12  +0.0 4,089,660 -  Psyr_3425 0.26  -0.3 4,089,694 +  Psyr_3425 0.30  -0.3 4,089,751 -  +0.8 4,090,095 +  Psyr_3425 0.85  +0.3 4,090,096 -  Psyr_3425 0.85  -0.6 4,090,099 +  Psyr_3425 0.86  +0.7 4,090,146 +  -0.8 4,090,146 +  +2.0 4,090,146 +  +0.8 4,090,146 +  +2.0 4,090,146 +  +0.6 4,090,147 -  -0.6 4,090,147 -  -0.3 4,090,147 -  -1.8 4,090,147 -  -0.8 4,090,231 +  -1.4 4,090,232 -  +0.0 4,090,410 +  Psyr_3426 0.26  -0.0 4,090,410 +  Psyr_3426 0.26  -0.6 4,090,540 +  Psyr_3426 0.50  +0.0 4,090,540 +  Psyr_3426 0.50  +0.7 4,090,540 +  Psyr_3426 0.50  +0.7 4,090,540 +  Psyr_3426 0.50  +0.5 4,090,540 +  Psyr_3426 0.50  -1.3 4,090,540 +  Psyr_3426 0.50  +0.7 4,090,541 -  Psyr_3426 0.50  -0.6 4,090,541 -  Psyr_3426 0.50  -0.8 4,090,541 -  Psyr_3426 0.50  -0.3 4,090,541 -  Psyr_3426 0.50  -0.1 4,090,541 -  Psyr_3426 0.50  +2.2 4,090,554 +  Psyr_3426 0.52  +0.4 4,090,567 -  Psyr_3426 0.55  +1.4 4,090,578 +  Psyr_3426 0.57  +0.8 4,090,578 +  Psyr_3426 0.57  +0.2 4,090,578 +  Psyr_3426 0.57  -0.6 4,090,705 -  Psyr_3426 0.80  -2.4 4,090,869 +  +0.9 4,090,870 -  +0.0 4,090,918 -  -0.6 4,090,918 -  -0.1 4,090,918 -  -0.8 4,091,097 +  -0.2 4,091,097 +  -1.3 4,091,098 -  +0.9 4,091,142 +  -0.3 4,091,142 +  +0.1 4,091,142 +  -0.4 4,091,143 -  -1.2 4,091,143 -  -0.4 
Or see this region's nucleotide sequence