Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2951 and Psyr_2952 are separated by 110 nucleotides Psyr_2952 and Psyr_2953 are separated by 30 nucleotides Psyr_2953 and Psyr_2954 are separated by 4 nucleotides
Psyr_2951: Psyr_2951 - transcriptional regulator, LysR family, at 3,539,799 to 3,540,698
_2951
Psyr_2952: Psyr_2952 - NADP oxidoreductase, coenzyme F420-dependent, at 3,540,809 to 3,541,438
_2952
Psyr_2953: Psyr_2953 - Catalytic LigB subunit of aromatic ring-opening dioxygenase, at 3,541,469 to 3,542,302
_2953
Psyr_2954: Psyr_2954 - Phospholipase/Carboxylesterase, at 3,542,307 to 3,542,978
_2954
Position (kb)
3540
3541
3542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3539.856 kb on + strand at 3539.857 kb on - strand at 3539.857 kb on - strand at 3539.897 kb on - strand, within Psyr_2951 at 3539.897 kb on - strand, within Psyr_2951 at 3539.897 kb on - strand, within Psyr_2951 at 3539.897 kb on - strand, within Psyr_2951 at 3539.897 kb on - strand, within Psyr_2951 at 3539.931 kb on + strand, within Psyr_2951 at 3539.931 kb on + strand, within Psyr_2951 at 3539.955 kb on + strand, within Psyr_2951 at 3539.973 kb on - strand, within Psyr_2951 at 3539.973 kb on - strand, within Psyr_2951 at 3539.973 kb on - strand, within Psyr_2951 at 3540.038 kb on + strand, within Psyr_2951 at 3540.038 kb on + strand, within Psyr_2951 at 3540.038 kb on + strand, within Psyr_2951 at 3540.038 kb on + strand, within Psyr_2951 at 3540.038 kb on + strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.039 kb on - strand, within Psyr_2951 at 3540.218 kb on + strand, within Psyr_2951 at 3540.251 kb on + strand, within Psyr_2951 at 3540.284 kb on + strand, within Psyr_2951 at 3540.284 kb on + strand, within Psyr_2951 at 3540.284 kb on + strand, within Psyr_2951 at 3540.285 kb on - strand, within Psyr_2951 at 3540.502 kb on + strand, within Psyr_2951 at 3540.502 kb on + strand, within Psyr_2951 at 3540.762 kb on - strand at 3540.762 kb on - strand at 3540.762 kb on - strand at 3540.764 kb on - strand at 3540.987 kb on + strand, within Psyr_2952 at 3540.987 kb on + strand, within Psyr_2952 at 3540.987 kb on + strand, within Psyr_2952 at 3540.988 kb on - strand, within Psyr_2952 at 3540.988 kb on - strand, within Psyr_2952 at 3541.054 kb on + strand, within Psyr_2952 at 3541.094 kb on + strand, within Psyr_2952 at 3541.094 kb on + strand, within Psyr_2952 at 3541.094 kb on + strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.095 kb on - strand, within Psyr_2952 at 3541.245 kb on + strand, within Psyr_2952 at 3541.246 kb on - strand, within Psyr_2952 at 3541.379 kb on + strand at 3541.379 kb on + strand at 3541.379 kb on + strand at 3541.380 kb on - strand at 3541.380 kb on - strand at 3541.436 kb on + strand at 3541.436 kb on + strand at 3541.436 kb on + strand at 3541.437 kb on - strand at 3541.437 kb on - strand at 3541.694 kb on + strand, within Psyr_2953 at 3541.694 kb on + strand, within Psyr_2953 at 3541.694 kb on + strand, within Psyr_2953 at 3541.695 kb on - strand, within Psyr_2953 at 3541.695 kb on - strand, within Psyr_2953 at 3541.695 kb on - strand, within Psyr_2953 at 3541.695 kb on - strand, within Psyr_2953 at 3541.695 kb on - strand, within Psyr_2953 at 3541.696 kb on + strand, within Psyr_2953 at 3541.696 kb on + strand, within Psyr_2953 at 3541.696 kb on + strand, within Psyr_2953 at 3541.696 kb on + strand, within Psyr_2953 at 3541.697 kb on - strand, within Psyr_2953 at 3541.697 kb on - strand, within Psyr_2953 at 3541.697 kb on - strand, within Psyr_2953 at 3541.697 kb on - strand, within Psyr_2953 at 3541.741 kb on + strand, within Psyr_2953 at 3541.741 kb on + strand, within Psyr_2953 at 3541.742 kb on - strand, within Psyr_2953 at 3541.742 kb on - strand, within Psyr_2953 at 3541.742 kb on - strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.825 kb on + strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.826 kb on - strand, within Psyr_2953 at 3541.829 kb on + strand, within Psyr_2953 at 3541.829 kb on + strand, within Psyr_2953 at 3541.830 kb on - strand, within Psyr_2953 at 3541.830 kb on - strand, within Psyr_2953 at 3541.830 kb on - strand, within Psyr_2953 at 3541.830 kb on - strand, within Psyr_2953 at 3541.830 kb on - strand, within Psyr_2953 at 3541.888 kb on + strand, within Psyr_2953 at 3541.944 kb on + strand, within Psyr_2953 at 3541.944 kb on + strand, within Psyr_2953 at 3541.945 kb on - strand, within Psyr_2953 at 3541.945 kb on - strand, within Psyr_2953 at 3541.983 kb on - strand, within Psyr_2953 at 3542.087 kb on + strand, within Psyr_2953 at 3542.097 kb on - strand, within Psyr_2953 at 3542.207 kb on - strand, within Psyr_2953 at 3542.210 kb on - strand, within Psyr_2953 at 3542.237 kb on + strand at 3542.237 kb on + strand at 3542.238 kb on - strand at 3542.238 kb on - strand at 3542.245 kb on + strand at 3542.300 kb on + strand at 3542.300 kb on + strand at 3542.375 kb on + strand, within Psyr_2954 at 3542.375 kb on + strand, within Psyr_2954 at 3542.375 kb on + strand, within Psyr_2954 at 3542.376 kb on - strand, within Psyr_2954 at 3542.376 kb on - strand, within Psyr_2954 at 3542.376 kb on - strand, within Psyr_2954 at 3542.376 kb on - strand, within Psyr_2954 at 3542.420 kb on - strand, within Psyr_2954
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 3,539,856 + -0.6 3,539,857 - +1.0 3,539,857 - -1.2 3,539,897 - Psyr_2951 0.11 -0.1 3,539,897 - Psyr_2951 0.11 -0.1 3,539,897 - Psyr_2951 0.11 +0.1 3,539,897 - Psyr_2951 0.11 -1.1 3,539,897 - Psyr_2951 0.11 +0.3 3,539,931 + Psyr_2951 0.15 -1.4 3,539,931 + Psyr_2951 0.15 -0.3 3,539,955 + Psyr_2951 0.17 -0.6 3,539,973 - Psyr_2951 0.19 +0.4 3,539,973 - Psyr_2951 0.19 -0.7 3,539,973 - Psyr_2951 0.19 +0.4 3,540,038 + Psyr_2951 0.27 -1.9 3,540,038 + Psyr_2951 0.27 -0.8 3,540,038 + Psyr_2951 0.27 -1.1 3,540,038 + Psyr_2951 0.27 +2.4 3,540,038 + Psyr_2951 0.27 +1.0 3,540,039 - Psyr_2951 0.27 +1.4 3,540,039 - Psyr_2951 0.27 -1.3 3,540,039 - Psyr_2951 0.27 -2.0 3,540,039 - Psyr_2951 0.27 -1.0 3,540,039 - Psyr_2951 0.27 -0.4 3,540,039 - Psyr_2951 0.27 -0.3 3,540,039 - Psyr_2951 0.27 -0.7 3,540,218 + Psyr_2951 0.47 -0.1 3,540,251 + Psyr_2951 0.50 +0.0 3,540,284 + Psyr_2951 0.54 -0.1 3,540,284 + Psyr_2951 0.54 +1.0 3,540,284 + Psyr_2951 0.54 -0.1 3,540,285 - Psyr_2951 0.54 -2.0 3,540,502 + Psyr_2951 0.78 -1.0 3,540,502 + Psyr_2951 0.78 +0.5 3,540,762 - +0.2 3,540,762 - +0.2 3,540,762 - +0.6 3,540,764 - +0.8 3,540,987 + Psyr_2952 0.28 +0.2 3,540,987 + Psyr_2952 0.28 -0.8 3,540,987 + Psyr_2952 0.28 -0.2 3,540,988 - Psyr_2952 0.28 -3.5 3,540,988 - Psyr_2952 0.28 -0.2 3,541,054 + Psyr_2952 0.39 +2.2 3,541,094 + Psyr_2952 0.45 -0.5 3,541,094 + Psyr_2952 0.45 +0.0 3,541,094 + Psyr_2952 0.45 -1.8 3,541,095 - Psyr_2952 0.45 +0.0 3,541,095 - Psyr_2952 0.45 +1.0 3,541,095 - Psyr_2952 0.45 -1.1 3,541,095 - Psyr_2952 0.45 -0.8 3,541,095 - Psyr_2952 0.45 -0.4 3,541,095 - Psyr_2952 0.45 -0.8 3,541,245 + Psyr_2952 0.69 -0.6 3,541,246 - Psyr_2952 0.69 +0.2 3,541,379 + -0.2 3,541,379 + -0.6 3,541,379 + +0.5 3,541,380 - -1.7 3,541,380 - +0.0 3,541,436 + -0.5 3,541,436 + -2.0 3,541,436 + -0.8 3,541,437 - +0.4 3,541,437 - -1.1 3,541,694 + Psyr_2953 0.27 -0.9 3,541,694 + Psyr_2953 0.27 -0.4 3,541,694 + Psyr_2953 0.27 +0.2 3,541,695 - Psyr_2953 0.27 -0.5 3,541,695 - Psyr_2953 0.27 +0.8 3,541,695 - Psyr_2953 0.27 -0.4 3,541,695 - Psyr_2953 0.27 +0.1 3,541,695 - Psyr_2953 0.27 +0.7 3,541,696 + Psyr_2953 0.27 +1.2 3,541,696 + Psyr_2953 0.27 -0.0 3,541,696 + Psyr_2953 0.27 -0.3 3,541,696 + Psyr_2953 0.27 -0.5 3,541,697 - Psyr_2953 0.27 +0.0 3,541,697 - Psyr_2953 0.27 -0.3 3,541,697 - Psyr_2953 0.27 -0.4 3,541,697 - Psyr_2953 0.27 +1.4 3,541,741 + Psyr_2953 0.33 -1.6 3,541,741 + Psyr_2953 0.33 -0.3 3,541,742 - Psyr_2953 0.33 +1.0 3,541,742 - Psyr_2953 0.33 +0.5 3,541,742 - Psyr_2953 0.33 +0.2 3,541,825 + Psyr_2953 0.43 -1.9 3,541,825 + Psyr_2953 0.43 -0.2 3,541,825 + Psyr_2953 0.43 -0.0 3,541,825 + Psyr_2953 0.43 -2.0 3,541,825 + Psyr_2953 0.43 -0.3 3,541,825 + Psyr_2953 0.43 -0.2 3,541,825 + Psyr_2953 0.43 +0.4 3,541,825 + Psyr_2953 0.43 -0.4 3,541,825 + Psyr_2953 0.43 -0.3 3,541,825 + Psyr_2953 0.43 +0.4 3,541,825 + Psyr_2953 0.43 +1.0 3,541,826 - Psyr_2953 0.43 +0.2 3,541,826 - Psyr_2953 0.43 +0.4 3,541,826 - Psyr_2953 0.43 +0.9 3,541,826 - Psyr_2953 0.43 +0.0 3,541,826 - Psyr_2953 0.43 -3.2 3,541,826 - Psyr_2953 0.43 +0.8 3,541,826 - Psyr_2953 0.43 +0.0 3,541,826 - Psyr_2953 0.43 -0.4 3,541,826 - Psyr_2953 0.43 -2.4 3,541,826 - Psyr_2953 0.43 +0.2 3,541,826 - Psyr_2953 0.43 -0.9 3,541,826 - Psyr_2953 0.43 +0.4 3,541,826 - Psyr_2953 0.43 -1.8 3,541,826 - Psyr_2953 0.43 +0.6 3,541,826 - Psyr_2953 0.43 -1.4 3,541,826 - Psyr_2953 0.43 +3.0 3,541,826 - Psyr_2953 0.43 -0.1 3,541,826 - Psyr_2953 0.43 -1.2 3,541,829 + Psyr_2953 0.43 -0.3 3,541,829 + Psyr_2953 0.43 +0.5 3,541,830 - Psyr_2953 0.43 +0.0 3,541,830 - Psyr_2953 0.43 +0.0 3,541,830 - Psyr_2953 0.43 -0.2 3,541,830 - Psyr_2953 0.43 +0.0 3,541,830 - Psyr_2953 0.43 +0.6 3,541,888 + Psyr_2953 0.50 -1.0 3,541,944 + Psyr_2953 0.57 +0.2 3,541,944 + Psyr_2953 0.57 -1.2 3,541,945 - Psyr_2953 0.57 -0.6 3,541,945 - Psyr_2953 0.57 +0.4 3,541,983 - Psyr_2953 0.62 -1.3 3,542,087 + Psyr_2953 0.74 -0.3 3,542,097 - Psyr_2953 0.75 -1.2 3,542,207 - Psyr_2953 0.88 -0.5 3,542,210 - Psyr_2953 0.89 +0.4 3,542,237 + +0.9 3,542,237 + +0.9 3,542,238 - -0.0 3,542,238 - -1.0 3,542,245 + -0.0 3,542,300 + -0.5 3,542,300 + +0.8 3,542,375 + Psyr_2954 0.10 -0.4 3,542,375 + Psyr_2954 0.10 +0.0 3,542,375 + Psyr_2954 0.10 -0.6 3,542,376 - Psyr_2954 0.10 -1.6 3,542,376 - Psyr_2954 0.10 -0.1 3,542,376 - Psyr_2954 0.10 +0.9 3,542,376 - Psyr_2954 0.10 -0.8 3,542,420 - Psyr_2954 0.17 -0.7
Or see this region's nucleotide sequence