Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2713 and Psyr_2714 are separated by 138 nucleotides Psyr_2714 and Psyr_2715 are separated by 388 nucleotides Psyr_2715 and Psyr_2716 are separated by 18 nucleotides
Psyr_2713: Psyr_2713 - transcriptional regulator, LysR family, at 3,295,137 to 3,296,036
_2713
Psyr_2714: Psyr_2714 - putative transmembrane protein, at 3,296,175 to 3,296,639
_2714
Psyr_2715: Psyr_2715 - Major facilitator superfamily, at 3,297,028 to 3,298,422
_2715
Psyr_2716: Psyr_2716 - Mandelate racemase/muconate lactonizing enzyme:Mandelate racemase/muconate lactonizing enzyme, at 3,298,441 to 3,299,715
_2716
Position (kb)
3297
3298
3299 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3296.077 kb on - strand at 3296.077 kb on - strand at 3296.082 kb on - strand at 3296.082 kb on - strand at 3296.082 kb on - strand at 3296.082 kb on - strand at 3296.137 kb on - strand at 3296.173 kb on + strand at 3296.174 kb on - strand at 3296.174 kb on - strand at 3296.174 kb on - strand at 3296.187 kb on - strand at 3296.187 kb on - strand at 3296.187 kb on - strand at 3296.210 kb on + strand at 3296.265 kb on - strand, within Psyr_2714 at 3296.268 kb on + strand, within Psyr_2714 at 3296.268 kb on + strand, within Psyr_2714 at 3296.268 kb on + strand, within Psyr_2714 at 3296.269 kb on - strand, within Psyr_2714 at 3296.369 kb on + strand, within Psyr_2714 at 3296.369 kb on + strand, within Psyr_2714 at 3296.370 kb on - strand, within Psyr_2714 at 3296.453 kb on - strand, within Psyr_2714 at 3296.453 kb on - strand, within Psyr_2714 at 3296.484 kb on + strand, within Psyr_2714 at 3296.484 kb on + strand, within Psyr_2714 at 3296.521 kb on + strand, within Psyr_2714 at 3296.521 kb on + strand, within Psyr_2714 at 3296.521 kb on + strand, within Psyr_2714 at 3296.521 kb on + strand, within Psyr_2714 at 3296.522 kb on - strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.525 kb on + strand, within Psyr_2714 at 3296.526 kb on - strand, within Psyr_2714 at 3296.549 kb on + strand, within Psyr_2714 at 3296.550 kb on - strand, within Psyr_2714 at 3296.615 kb on - strand at 3296.615 kb on - strand at 3296.627 kb on + strand at 3296.627 kb on + strand at 3296.628 kb on - strand at 3296.628 kb on - strand at 3296.691 kb on + strand at 3296.692 kb on - strand at 3296.692 kb on - strand at 3296.696 kb on - strand at 3296.696 kb on - strand at 3296.699 kb on + strand at 3296.699 kb on + strand at 3296.700 kb on - strand at 3296.700 kb on - strand at 3296.700 kb on - strand at 3296.721 kb on - strand at 3296.733 kb on - strand at 3296.788 kb on - strand at 3296.895 kb on + strand at 3296.895 kb on + strand at 3296.895 kb on + strand at 3296.895 kb on + strand at 3296.896 kb on - strand at 3296.896 kb on - strand at 3296.896 kb on - strand at 3296.932 kb on - strand at 3296.989 kb on - strand at 3297.064 kb on - strand at 3297.066 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.151 kb on + strand at 3297.152 kb on - strand at 3297.180 kb on - strand, within Psyr_2715 at 3297.388 kb on + strand, within Psyr_2715 at 3297.389 kb on - strand, within Psyr_2715 at 3297.389 kb on - strand, within Psyr_2715 at 3297.445 kb on + strand, within Psyr_2715 at 3297.445 kb on + strand, within Psyr_2715 at 3297.445 kb on + strand, within Psyr_2715 at 3297.445 kb on + strand, within Psyr_2715 at 3297.446 kb on - strand, within Psyr_2715 at 3297.446 kb on - strand, within Psyr_2715 at 3297.446 kb on - strand, within Psyr_2715 at 3297.446 kb on - strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.448 kb on + strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.449 kb on - strand, within Psyr_2715 at 3297.818 kb on - strand, within Psyr_2715 at 3297.841 kb on + strand, within Psyr_2715 at 3297.841 kb on + strand, within Psyr_2715 at 3297.841 kb on + strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.842 kb on - strand, within Psyr_2715 at 3297.952 kb on + strand, within Psyr_2715 at 3297.952 kb on + strand, within Psyr_2715 at 3297.952 kb on + strand, within Psyr_2715 at 3297.953 kb on - strand, within Psyr_2715 at 3297.953 kb on - strand, within Psyr_2715 at 3297.953 kb on - strand, within Psyr_2715 at 3298.565 kb on - strand at 3298.585 kb on + strand, within Psyr_2716 at 3298.585 kb on + strand, within Psyr_2716 at 3298.586 kb on - strand, within Psyr_2716 at 3298.586 kb on - strand, within Psyr_2716 at 3298.586 kb on - strand, within Psyr_2716 at 3298.701 kb on + strand, within Psyr_2716 at 3298.701 kb on + strand, within Psyr_2716 at 3298.702 kb on - strand, within Psyr_2716 at 3298.772 kb on - strand, within Psyr_2716 at 3298.772 kb on - strand, within Psyr_2716 at 3298.772 kb on - strand, within Psyr_2716 at 3298.788 kb on - strand, within Psyr_2716 at 3298.865 kb on + strand, within Psyr_2716 at 3298.865 kb on + strand, within Psyr_2716 at 3298.879 kb on + strand, within Psyr_2716 at 3298.879 kb on + strand, within Psyr_2716 at 3298.894 kb on + strand, within Psyr_2716 at 3298.895 kb on - strand, within Psyr_2716 at 3298.895 kb on - strand, within Psyr_2716 at 3298.993 kb on + strand, within Psyr_2716 at 3299.170 kb on + strand, within Psyr_2716 at 3299.170 kb on + strand, within Psyr_2716 at 3299.171 kb on - strand, within Psyr_2716 at 3299.174 kb on - strand, within Psyr_2716 at 3299.326 kb on + strand, within Psyr_2716
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 3,296,077 - +0.4 3,296,077 - -0.5 3,296,082 - -0.9 3,296,082 - -2.9 3,296,082 - -0.4 3,296,082 - +0.3 3,296,137 - +0.4 3,296,173 + -3.8 3,296,174 - -1.5 3,296,174 - -0.3 3,296,174 - +0.0 3,296,187 - -0.5 3,296,187 - +0.8 3,296,187 - +0.5 3,296,210 + -0.2 3,296,265 - Psyr_2714 0.19 -0.4 3,296,268 + Psyr_2714 0.20 -1.3 3,296,268 + Psyr_2714 0.20 +0.4 3,296,268 + Psyr_2714 0.20 -2.4 3,296,269 - Psyr_2714 0.20 +2.0 3,296,369 + Psyr_2714 0.42 +0.4 3,296,369 + Psyr_2714 0.42 +0.1 3,296,370 - Psyr_2714 0.42 -0.0 3,296,453 - Psyr_2714 0.60 -0.7 3,296,453 - Psyr_2714 0.60 +1.1 3,296,484 + Psyr_2714 0.66 +1.2 3,296,484 + Psyr_2714 0.66 -0.4 3,296,521 + Psyr_2714 0.74 +0.3 3,296,521 + Psyr_2714 0.74 -0.5 3,296,521 + Psyr_2714 0.74 -0.5 3,296,521 + Psyr_2714 0.74 -0.3 3,296,522 - Psyr_2714 0.75 +0.4 3,296,525 + Psyr_2714 0.75 -1.1 3,296,525 + Psyr_2714 0.75 -0.4 3,296,525 + Psyr_2714 0.75 -0.7 3,296,525 + Psyr_2714 0.75 +0.2 3,296,525 + Psyr_2714 0.75 -1.5 3,296,525 + Psyr_2714 0.75 -0.8 3,296,526 - Psyr_2714 0.75 +2.5 3,296,549 + Psyr_2714 0.80 +1.0 3,296,550 - Psyr_2714 0.81 -1.6 3,296,615 - +1.3 3,296,615 - -0.7 3,296,627 + +0.9 3,296,627 + -0.3 3,296,628 - -1.6 3,296,628 - +0.4 3,296,691 + +1.3 3,296,692 - -0.7 3,296,692 - +0.4 3,296,696 - +0.4 3,296,696 - +1.4 3,296,699 + +0.6 3,296,699 + -0.6 3,296,700 - +0.6 3,296,700 - +1.5 3,296,700 - -0.1 3,296,721 - -0.4 3,296,733 - +0.5 3,296,788 - +0.1 3,296,895 + -0.1 3,296,895 + +0.7 3,296,895 + +0.9 3,296,895 + +1.7 3,296,896 - -0.6 3,296,896 - +0.4 3,296,896 - -1.0 3,296,932 - +0.2 3,296,989 - -0.6 3,297,064 - +0.1 3,297,066 + +0.5 3,297,151 + -1.9 3,297,151 + -0.3 3,297,151 + -2.4 3,297,151 + -1.3 3,297,151 + -0.3 3,297,151 + +1.4 3,297,151 + +0.6 3,297,152 - -0.2 3,297,180 - Psyr_2715 0.11 +0.0 3,297,388 + Psyr_2715 0.26 -0.3 3,297,389 - Psyr_2715 0.26 -1.9 3,297,389 - Psyr_2715 0.26 -2.6 3,297,445 + Psyr_2715 0.30 +2.0 3,297,445 + Psyr_2715 0.30 +0.2 3,297,445 + Psyr_2715 0.30 -1.6 3,297,445 + Psyr_2715 0.30 -0.3 3,297,446 - Psyr_2715 0.30 +0.1 3,297,446 - Psyr_2715 0.30 -1.4 3,297,446 - Psyr_2715 0.30 +2.7 3,297,446 - Psyr_2715 0.30 -1.2 3,297,448 + Psyr_2715 0.30 +0.4 3,297,448 + Psyr_2715 0.30 +2.2 3,297,448 + Psyr_2715 0.30 -0.7 3,297,448 + Psyr_2715 0.30 +0.3 3,297,448 + Psyr_2715 0.30 -0.0 3,297,448 + Psyr_2715 0.30 -0.1 3,297,448 + Psyr_2715 0.30 -0.5 3,297,448 + Psyr_2715 0.30 -0.8 3,297,449 - Psyr_2715 0.30 -0.9 3,297,449 - Psyr_2715 0.30 +2.0 3,297,449 - Psyr_2715 0.30 -0.8 3,297,449 - Psyr_2715 0.30 +1.6 3,297,449 - Psyr_2715 0.30 +0.2 3,297,449 - Psyr_2715 0.30 +0.8 3,297,449 - Psyr_2715 0.30 -0.3 3,297,818 - Psyr_2715 0.57 -0.4 3,297,841 + Psyr_2715 0.58 -1.0 3,297,841 + Psyr_2715 0.58 +1.0 3,297,841 + Psyr_2715 0.58 -0.8 3,297,842 - Psyr_2715 0.58 -0.4 3,297,842 - Psyr_2715 0.58 +0.4 3,297,842 - Psyr_2715 0.58 -0.2 3,297,842 - +1.4 3,297,842 - Psyr_2715 0.58 -0.8 3,297,842 - Psyr_2715 0.58 +1.3 3,297,842 - Psyr_2715 0.58 +1.9 3,297,842 - Psyr_2715 0.58 -1.1 3,297,842 - Psyr_2715 0.58 +0.0 3,297,842 - Psyr_2715 0.58 -0.6 3,297,952 + Psyr_2715 0.66 -1.0 3,297,952 + Psyr_2715 0.66 +0.4 3,297,952 + Psyr_2715 0.66 -2.0 3,297,953 - Psyr_2715 0.66 -0.6 3,297,953 - Psyr_2715 0.66 +0.7 3,297,953 - Psyr_2715 0.66 -0.7 3,298,565 - +0.2 3,298,585 + Psyr_2716 0.11 +0.1 3,298,585 + Psyr_2716 0.11 +0.3 3,298,586 - Psyr_2716 0.11 -1.6 3,298,586 - Psyr_2716 0.11 +0.1 3,298,586 - Psyr_2716 0.11 +1.4 3,298,701 + Psyr_2716 0.20 +0.3 3,298,701 + Psyr_2716 0.20 -0.9 3,298,702 - Psyr_2716 0.20 -0.1 3,298,772 - Psyr_2716 0.26 -0.2 3,298,772 - Psyr_2716 0.26 -0.2 3,298,772 - Psyr_2716 0.26 -1.7 3,298,788 - Psyr_2716 0.27 -0.3 3,298,865 + Psyr_2716 0.33 +0.1 3,298,865 + Psyr_2716 0.33 -0.6 3,298,879 + Psyr_2716 0.34 -0.2 3,298,879 + Psyr_2716 0.34 +0.2 3,298,894 + Psyr_2716 0.36 -1.3 3,298,895 - Psyr_2716 0.36 -0.9 3,298,895 - Psyr_2716 0.36 -1.3 3,298,993 + Psyr_2716 0.43 -2.2 3,299,170 + Psyr_2716 0.57 -0.6 3,299,170 + Psyr_2716 0.57 -1.8 3,299,171 - Psyr_2716 0.57 -0.0 3,299,174 - Psyr_2716 0.57 -2.2 3,299,326 + Psyr_2716 0.69 +0.6
Or see this region's nucleotide sequence