Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0988 and Psyr_0989 are separated by 380 nucleotides Psyr_0989 and Psyr_0990 are separated by 169 nucleotides
Psyr_0988: Psyr_0988 - Integrase, catalytic region, at 1,118,184 to 1,118,966
_0988
Psyr_0989: Psyr_0989 - Lysine exporter protein (LYSE/YGGA), at 1,119,347 to 1,119,979
_0989
Psyr_0990: Psyr_0990 - AMP-dependent synthetase and ligase, at 1,120,149 to 1,121,831
_0990
Position (kb)
1119
1120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.971 kb on + strand at 1118.973 kb on + strand at 1118.973 kb on + strand at 1118.973 kb on + strand at 1118.973 kb on + strand at 1119.244 kb on + strand at 1119.244 kb on + strand at 1119.245 kb on - strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.249 kb on - strand at 1119.306 kb on + strand at 1119.306 kb on + strand at 1119.350 kb on + strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.591 kb on - strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.695 kb on + strand at 1119.695 kb on + strand, within Psyr_0989 at 1119.695 kb on + strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.815 kb on + strand, within Psyr_0989 at 1120.109 kb on + strand at 1120.110 kb on - strand at 1120.110 kb on - strand at 1120.110 kb on - strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.260 kb on + strand at 1120.260 kb on + strand at 1120.260 kb on + strand at 1120.261 kb on - strand at 1120.278 kb on + strand at 1120.284 kb on + strand at 1120.285 kb on - strand at 1120.306 kb on + strand at 1120.306 kb on + strand at 1120.307 kb on - strand at 1120.324 kb on + strand, within Psyr_0990 at 1120.324 kb on + strand, within Psyr_0990 at 1120.324 kb on + strand, within Psyr_0990 at 1120.389 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.534 kb on - strand, within Psyr_0990 at 1120.534 kb on - strand, within Psyr_0990 at 1120.621 kb on + strand, within Psyr_0990 at 1120.621 kb on + strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.632 kb on + strand, within Psyr_0990 at 1120.633 kb on - strand, within Psyr_0990 at 1120.633 kb on - strand, within Psyr_0990 at 1120.665 kb on + strand, within Psyr_0990 at 1120.666 kb on - strand, within Psyr_0990 at 1120.666 kb on - strand, within Psyr_0990 at 1120.710 kb on + strand, within Psyr_0990 at 1120.710 kb on + strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.747 kb on - strand, within Psyr_0990 at 1120.747 kb on - strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.923 kb on + strand, within Psyr_0990
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 1,118,971 + -0.3 1,118,971 + +0.2 1,118,971 + -0.7 1,118,971 + -0.4 1,118,971 + -0.1 1,118,971 + +0.2 1,118,971 + +0.8 1,118,971 + -0.4 1,118,973 + -0.8 1,118,973 + +0.1 1,118,973 + +0.3 1,118,973 + -1.0 1,119,244 + -0.0 1,119,244 + -1.2 1,119,245 - +0.1 1,119,248 + -1.3 1,119,248 + +0.6 1,119,248 + +2.4 1,119,248 + -1.7 1,119,248 + -0.3 1,119,249 - -0.8 1,119,306 + -0.3 1,119,306 + -0.0 1,119,350 + +0.2 1,119,351 - +0.4 1,119,351 - -1.9 1,119,351 - +0.5 1,119,351 - +0.0 1,119,591 - Psyr_0989 0.39 -0.1 1,119,594 + Psyr_0989 0.39 -0.7 1,119,594 + Psyr_0989 0.39 +1.9 1,119,594 + Psyr_0989 0.39 -1.1 1,119,594 + Psyr_0989 0.39 +0.2 1,119,594 + Psyr_0989 0.39 -0.9 1,119,594 + Psyr_0989 0.39 -1.0 1,119,595 - Psyr_0989 0.39 -0.6 1,119,595 - Psyr_0989 0.39 -1.3 1,119,595 - Psyr_0989 0.39 -0.2 1,119,595 - Psyr_0989 0.39 +0.3 1,119,595 - Psyr_0989 0.39 +0.1 1,119,595 - Psyr_0989 0.39 -1.4 1,119,595 - Psyr_0989 0.39 -0.5 1,119,595 - Psyr_0989 0.39 -0.7 1,119,595 - Psyr_0989 0.39 +1.6 1,119,595 - Psyr_0989 0.39 -0.9 1,119,595 - Psyr_0989 0.39 +0.6 1,119,599 + Psyr_0989 0.40 -0.1 1,119,599 + Psyr_0989 0.40 -0.4 1,119,599 + Psyr_0989 0.40 -0.8 1,119,600 - Psyr_0989 0.40 +2.2 1,119,600 - Psyr_0989 0.40 +0.0 1,119,600 - Psyr_0989 0.40 -0.5 1,119,600 - Psyr_0989 0.40 -0.6 1,119,600 - Psyr_0989 0.40 -0.0 1,119,600 - Psyr_0989 0.40 -2.7 1,119,600 - Psyr_0989 0.40 -0.0 1,119,695 + -0.4 1,119,695 + Psyr_0989 0.55 -0.4 1,119,695 + Psyr_0989 0.55 +0.1 1,119,696 - Psyr_0989 0.55 +0.1 1,119,696 - Psyr_0989 0.55 -0.6 1,119,696 - Psyr_0989 0.55 -0.9 1,119,696 - Psyr_0989 0.55 -0.5 1,119,696 - Psyr_0989 0.55 -1.1 1,119,815 + Psyr_0989 0.74 -0.7 1,120,109 + -0.1 1,120,110 - -0.9 1,120,110 - +0.2 1,120,110 - -0.2 1,120,148 + +0.3 1,120,148 + +1.0 1,120,148 + +0.0 1,120,148 + -0.1 1,120,148 + -0.6 1,120,149 - -0.2 1,120,149 - -0.0 1,120,149 - -1.0 1,120,149 - +0.3 1,120,149 - -0.8 1,120,149 - -0.6 1,120,149 - +1.6 1,120,149 - +1.7 1,120,260 + -1.2 1,120,260 + -0.7 1,120,260 + -1.9 1,120,261 - -1.1 1,120,278 + -0.4 1,120,284 + +0.6 1,120,285 - +0.3 1,120,306 + -0.9 1,120,306 + -0.1 1,120,307 - -0.6 1,120,324 + Psyr_0990 0.10 +0.7 1,120,324 + Psyr_0990 0.10 +0.1 1,120,324 + Psyr_0990 0.10 -0.3 1,120,389 + Psyr_0990 0.14 -0.4 1,120,533 + Psyr_0990 0.23 -0.0 1,120,533 + Psyr_0990 0.23 +0.2 1,120,533 + Psyr_0990 0.23 +1.2 1,120,534 - Psyr_0990 0.23 -2.2 1,120,534 - Psyr_0990 0.23 +1.5 1,120,621 + Psyr_0990 0.28 -0.3 1,120,621 + Psyr_0990 0.28 +0.2 1,120,622 - Psyr_0990 0.28 +1.3 1,120,622 - Psyr_0990 0.28 -0.1 1,120,622 - Psyr_0990 0.28 -1.2 1,120,632 + Psyr_0990 0.29 -1.2 1,120,633 - Psyr_0990 0.29 -0.0 1,120,633 - Psyr_0990 0.29 -0.0 1,120,665 + Psyr_0990 0.31 -2.8 1,120,666 - Psyr_0990 0.31 +0.1 1,120,666 - Psyr_0990 0.31 +1.4 1,120,710 + Psyr_0990 0.33 -0.4 1,120,710 + Psyr_0990 0.33 +0.6 1,120,711 - Psyr_0990 0.33 +0.7 1,120,711 - Psyr_0990 0.33 -0.4 1,120,711 - Psyr_0990 0.33 +0.4 1,120,711 - Psyr_0990 0.33 -0.6 1,120,746 + Psyr_0990 0.35 -2.4 1,120,746 + Psyr_0990 0.35 -0.2 1,120,746 + Psyr_0990 0.35 +0.0 1,120,746 + Psyr_0990 0.35 -0.0 1,120,746 + Psyr_0990 0.35 +1.3 1,120,746 + Psyr_0990 0.35 +0.5 1,120,747 - Psyr_0990 0.36 +1.2 1,120,747 - Psyr_0990 0.36 +0.1 1,120,842 + Psyr_0990 0.41 -1.2 1,120,842 + Psyr_0990 0.41 +1.1 1,120,842 + Psyr_0990 0.41 -0.3 1,120,842 + Psyr_0990 0.41 -2.5 1,120,842 + Psyr_0990 0.41 +1.8 1,120,842 + Psyr_0990 0.41 +0.6 1,120,843 - Psyr_0990 0.41 -1.3 1,120,843 - Psyr_0990 0.41 -1.0 1,120,843 - Psyr_0990 0.41 +0.1 1,120,843 - Psyr_0990 0.41 +0.6 1,120,843 - Psyr_0990 0.41 -0.2 1,120,843 - Psyr_0990 0.41 +0.3 1,120,843 - Psyr_0990 0.41 +0.2 1,120,843 - Psyr_0990 0.41 +0.2 1,120,923 + Psyr_0990 0.46 +0.1
Or see this region's nucleotide sequence