Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS31595

Experiment: Growth with Chloride 200 mM; with MOPS

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPFLU_RS13270 and PFLU_RS13275 are separated by 73 nucleotidesPFLU_RS13275 and PFLU_RS31595 are separated by 120 nucleotidesPFLU_RS31595 and PFLU_RS13280 are separated by 217 nucleotides PFLU_RS13270: PFLU_RS13270 - AraC family transcriptional regulator, at 2,999,829 to 3,000,668 _RS13270 PFLU_RS13275: PFLU_RS13275 - DUF1345 domain-containing protein, at 3,000,742 to 3,001,383 _RS13275 PFLU_RS31595: PFLU_RS31595 - hypothetical protein, at 3,001,504 to 3,001,773 _RS31595 PFLU_RS13280: PFLU_RS13280 - multidrug efflux RND transporter permease subunit, at 3,001,991 to 3,005,083 _RS13280 Position (kb) 3001 3002Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3000.559 kb on + strand, within PFLU_RS13270at 3000.559 kb on + strand, within PFLU_RS13270at 3000.559 kb on + strand, within PFLU_RS13270at 3000.559 kb on + strand, within PFLU_RS13270at 3000.560 kb on - strand, within PFLU_RS13270at 3000.583 kb on + strand, within PFLU_RS13270at 3000.583 kb on + strand, within PFLU_RS13270at 3000.583 kb on + strand, within PFLU_RS13270at 3000.583 kb on + strand, within PFLU_RS13270at 3000.584 kb on - strand, within PFLU_RS13270at 3000.584 kb on - strand, within PFLU_RS13270at 3000.584 kb on - strand, within PFLU_RS13270at 3000.584 kb on - strand, within PFLU_RS13270at 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.686 kb on + strandat 3000.696 kb on + strandat 3000.696 kb on + strandat 3000.696 kb on + strandat 3000.697 kb on - strandat 3000.697 kb on - strandat 3000.697 kb on - strandat 3000.699 kb on + strandat 3000.724 kb on + strandat 3000.724 kb on + strandat 3000.725 kb on - strandat 3000.725 kb on - strandat 3000.859 kb on + strand, within PFLU_RS13275at 3000.859 kb on + strand, within PFLU_RS13275at 3000.859 kb on + strand, within PFLU_RS13275at 3000.859 kb on + strand, within PFLU_RS13275at 3000.860 kb on - strand, within PFLU_RS13275at 3000.902 kb on + strand, within PFLU_RS13275at 3000.902 kb on + strand, within PFLU_RS13275at 3000.917 kb on + strand, within PFLU_RS13275at 3000.917 kb on + strand, within PFLU_RS13275at 3000.917 kb on + strand, within PFLU_RS13275at 3000.917 kb on + strand, within PFLU_RS13275at 3000.918 kb on - strand, within PFLU_RS13275at 3000.977 kb on + strand, within PFLU_RS13275at 3000.977 kb on + strand, within PFLU_RS13275at 3000.977 kb on + strand, within PFLU_RS13275at 3000.977 kb on + strand, within PFLU_RS13275at 3000.977 kb on + strand, within PFLU_RS13275at 3000.978 kb on - strand, within PFLU_RS13275at 3000.980 kb on + strand, within PFLU_RS13275at 3000.980 kb on + strand, within PFLU_RS13275at 3000.980 kb on + strand, within PFLU_RS13275at 3000.981 kb on - strand, within PFLU_RS13275at 3000.981 kb on - strand, within PFLU_RS13275at 3000.993 kb on - strand, within PFLU_RS13275at 3001.050 kb on + strand, within PFLU_RS13275at 3001.250 kb on + strand, within PFLU_RS13275at 3001.250 kb on + strand, within PFLU_RS13275at 3001.250 kb on + strand, within PFLU_RS13275at 3001.250 kb on + strand, within PFLU_RS13275at 3001.250 kb on + strand, within PFLU_RS13275at 3001.251 kb on - strand, within PFLU_RS13275at 3001.251 kb on - strand, within PFLU_RS13275at 3001.251 kb on - strand, within PFLU_RS13275at 3001.251 kb on - strand, within PFLU_RS13275at 3001.383 kb on + strandat 3001.383 kb on + strandat 3001.383 kb on + strandat 3001.384 kb on - strandat 3001.384 kb on - strandat 3001.384 kb on - strandat 3001.384 kb on - strandat 3001.384 kb on - strandat 3001.411 kb on + strandat 3001.411 kb on + strandat 3001.411 kb on + strandat 3001.412 kb on - strandat 3001.412 kb on - strandat 3001.412 kb on - strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.418 kb on + strandat 3001.419 kb on - strandat 3001.419 kb on - strandat 3001.432 kb on + strandat 3001.432 kb on + strandat 3001.432 kb on + strandat 3001.432 kb on + strandat 3001.432 kb on + strandat 3001.433 kb on - strandat 3001.433 kb on - strandat 3001.433 kb on - strandat 3001.597 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.601 kb on + strand, within PFLU_RS31595at 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.928 kb on + strandat 3001.929 kb on - strandat 3001.929 kb on - strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.940 kb on + strandat 3001.948 kb on + strandat 3001.948 kb on + strandat 3001.948 kb on + strandat 3001.948 kb on + strandat 3001.948 kb on + strandat 3001.949 kb on - strandat 3001.949 kb on - strandat 3001.982 kb on + strandat 3001.982 kb on + strandat 3001.982 kb on + strandat 3001.983 kb on - strandat 3001.983 kb on - strandat 3001.993 kb on - strandat 3001.993 kb on - strandat 3001.993 kb on - strandat 3001.993 kb on - strandat 3002.244 kb on + strandat 3002.244 kb on + strandat 3002.245 kb on - strandat 3002.245 kb on - strandat 3002.245 kb on - strandat 3002.245 kb on - strandat 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.427 kb on + strand, within PFLU_RS13280at 3002.428 kb on - strand, within PFLU_RS13280at 3002.428 kb on - strand, within PFLU_RS13280at 3002.428 kb on - strand, within PFLU_RS13280at 3002.428 kb on - strand, within PFLU_RS13280

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Growth with Chloride 200 mM; with MOPS
remove
3,000,559 + PFLU_RS13270 0.87 +1.4
3,000,559 + PFLU_RS13270 0.87 +1.9
3,000,559 + PFLU_RS13270 0.87 +2.3
3,000,559 + PFLU_RS13270 0.87 -0.2
3,000,560 - PFLU_RS13270 0.87 -2.5
3,000,583 + PFLU_RS13270 0.90 -0.2
3,000,583 + PFLU_RS13270 0.90 +1.6
3,000,583 + PFLU_RS13270 0.90 -0.0
3,000,583 + PFLU_RS13270 0.90 +1.4
3,000,584 - PFLU_RS13270 0.90 -1.2
3,000,584 - PFLU_RS13270 0.90 -2.2
3,000,584 - PFLU_RS13270 0.90 +0.3
3,000,584 - PFLU_RS13270 0.90 +0.3
3,000,686 + +0.2
3,000,686 + -0.3
3,000,686 + +0.2
3,000,686 + -1.7
3,000,686 + -0.8
3,000,686 + -0.1
3,000,686 + -0.4
3,000,686 + -1.4
3,000,696 + -2.0
3,000,696 + -0.3
3,000,696 + -0.8
3,000,697 - +1.2
3,000,697 - -3.4
3,000,697 - +0.3
3,000,699 + +0.2
3,000,724 + +0.2
3,000,724 + -0.4
3,000,725 - -0.0
3,000,725 - +0.7
3,000,859 + PFLU_RS13275 0.18 -1.3
3,000,859 + PFLU_RS13275 0.18 +0.4
3,000,859 + PFLU_RS13275 0.18 -2.0
3,000,859 + PFLU_RS13275 0.18 -0.5
3,000,860 - PFLU_RS13275 0.18 -1.2
3,000,902 + PFLU_RS13275 0.25 +1.1
3,000,902 + PFLU_RS13275 0.25 +0.8
3,000,917 + PFLU_RS13275 0.27 -0.4
3,000,917 + PFLU_RS13275 0.27 -0.2
3,000,917 + PFLU_RS13275 0.27 +1.1
3,000,917 + PFLU_RS13275 0.27 -2.0
3,000,918 - PFLU_RS13275 0.27 +0.8
3,000,977 + PFLU_RS13275 0.37 +0.5
3,000,977 + PFLU_RS13275 0.37 -3.1
3,000,977 + PFLU_RS13275 0.37 +0.1
3,000,977 + PFLU_RS13275 0.37 -0.0
3,000,977 + PFLU_RS13275 0.37 +0.1
3,000,978 - PFLU_RS13275 0.37 +1.0
3,000,980 + PFLU_RS13275 0.37 -1.8
3,000,980 + PFLU_RS13275 0.37 +1.1
3,000,980 + PFLU_RS13275 0.37 +1.7
3,000,981 - PFLU_RS13275 0.37 -0.2
3,000,981 - PFLU_RS13275 0.37 -0.9
3,000,993 - PFLU_RS13275 0.39 -0.2
3,001,050 + PFLU_RS13275 0.48 +0.8
3,001,250 + PFLU_RS13275 0.79 +0.1
3,001,250 + PFLU_RS13275 0.79 -0.7
3,001,250 + PFLU_RS13275 0.79 +0.4
3,001,250 + PFLU_RS13275 0.79 -0.2
3,001,250 + PFLU_RS13275 0.79 +1.0
3,001,251 - PFLU_RS13275 0.79 -0.2
3,001,251 - PFLU_RS13275 0.79 +0.1
3,001,251 - PFLU_RS13275 0.79 +1.8
3,001,251 - PFLU_RS13275 0.79 +0.8
3,001,383 + -0.5
3,001,383 + +0.3
3,001,383 + -0.2
3,001,384 - +1.5
3,001,384 - -0.8
3,001,384 - -0.4
3,001,384 - -1.3
3,001,384 - +0.1
3,001,411 + +1.5
3,001,411 + +0.9
3,001,411 + +2.4
3,001,412 - -0.8
3,001,412 - +1.8
3,001,412 - -1.8
3,001,418 + +1.5
3,001,418 + +1.3
3,001,418 + -2.0
3,001,418 + +0.8
3,001,418 + +0.3
3,001,418 + +0.5
3,001,418 + -1.5
3,001,418 + +0.3
3,001,418 + +1.4
3,001,418 + -1.8
3,001,419 - -0.5
3,001,419 - -1.2
3,001,432 + -0.8
3,001,432 + -0.5
3,001,432 + +0.2
3,001,432 + -1.2
3,001,432 + +0.8
3,001,433 - +2.8
3,001,433 - +1.8
3,001,433 - -0.7
3,001,597 + PFLU_RS31595 0.34 -1.2
3,001,601 + PFLU_RS31595 0.36 -2.5
3,001,601 + PFLU_RS31595 0.36 -0.5
3,001,601 + PFLU_RS31595 0.36 -1.8
3,001,601 + PFLU_RS31595 0.36 -2.0
3,001,601 + PFLU_RS31595 0.36 -0.0
3,001,601 + PFLU_RS31595 0.36 +0.3
3,001,601 + PFLU_RS31595 0.36 -0.5
3,001,601 + PFLU_RS31595 0.36 -0.8
3,001,928 + +0.4
3,001,928 + +1.1
3,001,928 + +0.5
3,001,928 + +0.3
3,001,928 + +1.2
3,001,928 + +0.2
3,001,928 + +0.5
3,001,929 - -0.4
3,001,929 - +0.2
3,001,940 + +0.2
3,001,940 + -0.1
3,001,940 + -1.4
3,001,940 + +1.5
3,001,940 + +1.1
3,001,940 + -0.9
3,001,940 + +0.2
3,001,940 + -2.0
3,001,940 + -1.4
3,001,948 + +1.7
3,001,948 + +1.3
3,001,948 + -0.6
3,001,948 + -2.2
3,001,948 + -1.2
3,001,949 - -2.2
3,001,949 - -0.3
3,001,982 + +0.8
3,001,982 + -3.1
3,001,982 + +2.4
3,001,983 - -2.7
3,001,983 - +2.6
3,001,993 - +1.0
3,001,993 - +0.3
3,001,993 - +0.9
3,001,993 - -0.0
3,002,244 + +0.7
3,002,244 + -0.5
3,002,245 - +1.6
3,002,245 - +4.1
3,002,245 - -0.1
3,002,245 - +1.4
3,002,427 + PFLU_RS13280 0.14 +0.3
3,002,427 + PFLU_RS13280 0.14 +0.4
3,002,427 + PFLU_RS13280 0.14 +1.2
3,002,427 + PFLU_RS13280 0.14 -1.2
3,002,427 + PFLU_RS13280 0.14 +0.2
3,002,427 + PFLU_RS13280 0.14 +1.2
3,002,427 + PFLU_RS13280 0.14 +0.9
3,002,427 + PFLU_RS13280 0.14 +0.1
3,002,427 + PFLU_RS13280 0.14 -0.2
3,002,427 + PFLU_RS13280 0.14 +0.8
3,002,428 - PFLU_RS13280 0.14 -0.2
3,002,428 - PFLU_RS13280 0.14 -0.4
3,002,428 - PFLU_RS13280 0.14 -2.5
3,002,428 - PFLU_RS13280 0.14 -0.0

Or see this region's nucleotide sequence