Experiment: Growth with Chloride 200 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS25110 and PFLU_RS25115 are separated by 0 nucleotides PFLU_RS25115 and PFLU_RS25120 are separated by 182 nucleotides PFLU_RS25120 and PFLU_RS25125 are separated by 195 nucleotides PFLU_RS25125 and PFLU_RS25130 overlap by 4 nucleotides
PFLU_RS25110: PFLU_RS25110 - LTA synthase family protein, at 5,613,454 to 5,615,475
_RS25110
PFLU_RS25115: PFLU_RS25115 - cytochrome c family protein, at 5,615,476 to 5,615,850
_RS25115
PFLU_RS25120: PFLU_RS25120 - response regulator, at 5,616,033 to 5,616,413
_RS25120
PFLU_RS25125: PFLU_RS25125 - biliverdin-producing heme oxygenase, at 5,616,609 to 5,617,223
_RS25125
PFLU_RS25130: PFLU_RS25130 - GAF domain-containing protein, at 5,617,220 to 5,619,463
_RS25130
Position (kb) 5616
5617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.070 kb on + strand, within PFLU_RS25110 at 5615.071 kb on - strand, within PFLU_RS25110 at 5615.071 kb on - strand, within PFLU_RS25110 at 5615.071 kb on - strand, within PFLU_RS25110 at 5615.073 kb on + strand, within PFLU_RS25110 at 5615.073 kb on + strand, within PFLU_RS25110 at 5615.074 kb on - strand, within PFLU_RS25110 at 5615.074 kb on - strand, within PFLU_RS25110 at 5615.074 kb on - strand, within PFLU_RS25110 at 5615.400 kb on + strand at 5615.401 kb on - strand at 5615.401 kb on - strand at 5615.401 kb on - strand at 5615.401 kb on - strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.477 kb on + strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.478 kb on - strand at 5615.501 kb on + strand at 5615.501 kb on + strand at 5615.501 kb on + strand at 5615.502 kb on - strand at 5615.502 kb on - strand at 5615.591 kb on + strand, within PFLU_RS25115 at 5615.591 kb on + strand, within PFLU_RS25115 at 5615.592 kb on - strand, within PFLU_RS25115 at 5615.648 kb on + strand, within PFLU_RS25115 at 5615.648 kb on + strand, within PFLU_RS25115 at 5615.648 kb on + strand, within PFLU_RS25115 at 5615.649 kb on - strand, within PFLU_RS25115 at 5615.654 kb on + strand, within PFLU_RS25115 at 5615.654 kb on + strand, within PFLU_RS25115 at 5615.655 kb on - strand, within PFLU_RS25115 at 5615.655 kb on - strand, within PFLU_RS25115 at 5615.818 kb on + strand at 5615.818 kb on + strand at 5615.819 kb on - strand at 5615.836 kb on + strand at 5615.836 kb on + strand at 5615.837 kb on - strand at 5615.837 kb on - strand at 5615.837 kb on - strand at 5615.837 kb on - strand at 5615.843 kb on + strand at 5615.843 kb on + strand at 5615.843 kb on + strand at 5615.844 kb on - strand at 5615.883 kb on + strand at 5615.883 kb on + strand at 5615.883 kb on + strand at 5615.883 kb on + strand at 5615.883 kb on + strand at 5615.884 kb on - strand at 5615.884 kb on - strand at 5615.891 kb on + strand at 5615.927 kb on + strand at 5615.928 kb on - strand at 5615.978 kb on + strand at 5615.978 kb on + strand at 5616.003 kb on + strand at 5616.003 kb on + strand at 5616.004 kb on - strand at 5616.025 kb on + strand at 5616.025 kb on + strand at 5616.026 kb on - strand at 5616.026 kb on - strand at 5616.056 kb on - strand at 5616.156 kb on + strand, within PFLU_RS25120 at 5616.156 kb on + strand, within PFLU_RS25120 at 5616.157 kb on - strand, within PFLU_RS25120 at 5616.157 kb on - strand, within PFLU_RS25120 at 5616.291 kb on - strand, within PFLU_RS25120 at 5616.414 kb on + strand at 5616.415 kb on - strand at 5616.415 kb on - strand at 5616.415 kb on - strand at 5616.415 kb on - strand at 5616.415 kb on - strand at 5616.465 kb on + strand at 5616.465 kb on + strand at 5616.466 kb on - strand at 5616.466 kb on - strand at 5616.749 kb on - strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.759 kb on + strand, within PFLU_RS25125 at 5616.760 kb on - strand, within PFLU_RS25125 at 5616.760 kb on - strand, within PFLU_RS25125 at 5616.760 kb on - strand, within PFLU_RS25125 at 5616.760 kb on - strand, within PFLU_RS25125 at 5616.760 kb on - strand, within PFLU_RS25125 at 5616.762 kb on + strand, within PFLU_RS25125 at 5616.762 kb on + strand, within PFLU_RS25125 at 5616.762 kb on + strand, within PFLU_RS25125 at 5616.763 kb on - strand, within PFLU_RS25125 at 5616.771 kb on + strand, within PFLU_RS25125 at 5616.771 kb on + strand, within PFLU_RS25125 at 5616.772 kb on - strand, within PFLU_RS25125 at 5616.790 kb on + strand, within PFLU_RS25125 at 5616.791 kb on - strand, within PFLU_RS25125 at 5616.791 kb on - strand, within PFLU_RS25125 at 5616.795 kb on + strand, within PFLU_RS25125 at 5616.795 kb on + strand, within PFLU_RS25125 at 5616.795 kb on + strand, within PFLU_RS25125 at 5616.795 kb on + strand, within PFLU_RS25125 at 5616.796 kb on - strand, within PFLU_RS25125 at 5616.796 kb on - strand, within PFLU_RS25125 at 5616.796 kb on - strand, within PFLU_RS25125 at 5616.796 kb on - strand, within PFLU_RS25125 at 5616.796 kb on - strand, within PFLU_RS25125 at 5616.851 kb on + strand, within PFLU_RS25125 at 5616.851 kb on + strand, within PFLU_RS25125 at 5616.852 kb on - strand, within PFLU_RS25125 at 5616.897 kb on - strand, within PFLU_RS25125 at 5616.938 kb on + strand, within PFLU_RS25125 at 5616.939 kb on - strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.966 kb on + strand, within PFLU_RS25125 at 5616.967 kb on - strand, within PFLU_RS25125 at 5616.967 kb on - strand, within PFLU_RS25125 at 5616.967 kb on - strand, within PFLU_RS25125 at 5616.967 kb on - strand, within PFLU_RS25125 at 5616.967 kb on - strand, within PFLU_RS25125 at 5617.000 kb on + strand, within PFLU_RS25125 at 5617.001 kb on - strand, within PFLU_RS25125 at 5617.001 kb on - strand, within PFLU_RS25125 at 5617.001 kb on - strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.066 kb on + strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.067 kb on - strand, within PFLU_RS25125 at 5617.068 kb on + strand, within PFLU_RS25125 at 5617.068 kb on + strand, within PFLU_RS25125 at 5617.069 kb on - strand, within PFLU_RS25125 at 5617.110 kb on + strand, within PFLU_RS25125 at 5617.110 kb on + strand, within PFLU_RS25125 at 5617.111 kb on - strand, within PFLU_RS25125 at 5617.111 kb on - strand, within PFLU_RS25125 at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.213 kb on + strand at 5617.214 kb on - strand at 5617.214 kb on - strand at 5617.214 kb on - strand at 5617.214 kb on - strand at 5617.214 kb on - strand at 5617.214 kb on - strand at 5617.219 kb on + strand at 5617.219 kb on + strand at 5617.219 kb on + strand at 5617.219 kb on + strand at 5617.219 kb on + strand at 5617.219 kb on + strand at 5617.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 200 mM; with MOPS remove 5,615,070 + PFLU_RS25110 0.80 -0.3 5,615,070 + PFLU_RS25110 0.80 +0.4 5,615,070 + PFLU_RS25110 0.80 +0.4 5,615,070 + PFLU_RS25110 0.80 +0.7 5,615,070 + PFLU_RS25110 0.80 +0.8 5,615,070 + PFLU_RS25110 0.80 +1.3 5,615,070 + PFLU_RS25110 0.80 +1.1 5,615,070 + PFLU_RS25110 0.80 -1.3 5,615,071 - PFLU_RS25110 0.80 +0.3 5,615,071 - PFLU_RS25110 0.80 -1.5 5,615,071 - PFLU_RS25110 0.80 -0.4 5,615,073 + PFLU_RS25110 0.80 +0.5 5,615,073 + PFLU_RS25110 0.80 -0.3 5,615,074 - PFLU_RS25110 0.80 +0.5 5,615,074 - PFLU_RS25110 0.80 -0.1 5,615,074 - PFLU_RS25110 0.80 +2.3 5,615,400 + -0.0 5,615,401 - -0.0 5,615,401 - -0.0 5,615,401 - +1.0 5,615,401 - -0.3 5,615,477 + -0.4 5,615,477 + -1.3 5,615,477 + +1.5 5,615,477 + -0.5 5,615,477 + -1.3 5,615,477 + +0.1 5,615,477 + +0.8 5,615,477 + +1.0 5,615,477 + +1.5 5,615,477 + -2.2 5,615,478 - -0.3 5,615,478 - +2.0 5,615,478 - +0.4 5,615,478 - +2.3 5,615,478 - -3.3 5,615,478 - -1.8 5,615,478 - -2.4 5,615,478 - +0.7 5,615,501 + -2.0 5,615,501 + +0.9 5,615,501 + +0.3 5,615,502 - -2.3 5,615,502 - +0.4 5,615,591 + PFLU_RS25115 0.31 -0.1 5,615,591 + PFLU_RS25115 0.31 -1.0 5,615,592 - PFLU_RS25115 0.31 +0.2 5,615,648 + PFLU_RS25115 0.46 -2.8 5,615,648 + PFLU_RS25115 0.46 +0.7 5,615,648 + PFLU_RS25115 0.46 +1.0 5,615,649 - PFLU_RS25115 0.46 +1.7 5,615,654 + PFLU_RS25115 0.47 +1.5 5,615,654 + PFLU_RS25115 0.47 +2.9 5,615,655 - PFLU_RS25115 0.48 +2.2 5,615,655 - PFLU_RS25115 0.48 -0.2 5,615,818 + -0.4 5,615,818 + -0.1 5,615,819 - +1.2 5,615,836 + +0.2 5,615,836 + -0.1 5,615,837 - -1.6 5,615,837 - +0.7 5,615,837 - +0.1 5,615,837 - -2.3 5,615,843 + -0.9 5,615,843 + +0.1 5,615,843 + -1.0 5,615,844 - -0.2 5,615,883 + -1.4 5,615,883 + -0.9 5,615,883 + +0.6 5,615,883 + +0.8 5,615,883 + +1.7 5,615,884 - +1.3 5,615,884 - +1.5 5,615,891 + -1.8 5,615,927 + +0.0 5,615,928 - -1.2 5,615,978 + +0.2 5,615,978 + -2.0 5,616,003 + -1.3 5,616,003 + +1.2 5,616,004 - -0.1 5,616,025 + +0.4 5,616,025 + +0.9 5,616,026 - +2.8 5,616,026 - -1.8 5,616,056 - +0.5 5,616,156 + PFLU_RS25120 0.32 -1.8 5,616,156 + PFLU_RS25120 0.32 -0.1 5,616,157 - PFLU_RS25120 0.33 -2.2 5,616,157 - PFLU_RS25120 0.33 -2.4 5,616,291 - PFLU_RS25120 0.68 -0.5 5,616,414 + +1.1 5,616,415 - +0.1 5,616,415 - -0.9 5,616,415 - -0.3 5,616,415 - -1.9 5,616,415 - -0.1 5,616,465 + +0.7 5,616,465 + -2.7 5,616,466 - -0.9 5,616,466 - -0.9 5,616,749 - PFLU_RS25125 0.23 -0.4 5,616,759 + PFLU_RS25125 0.24 +0.2 5,616,759 + PFLU_RS25125 0.24 -0.1 5,616,759 + PFLU_RS25125 0.24 +1.4 5,616,759 + PFLU_RS25125 0.24 -1.1 5,616,759 + PFLU_RS25125 0.24 -0.5 5,616,759 + PFLU_RS25125 0.24 +1.4 5,616,759 + PFLU_RS25125 0.24 +1.2 5,616,760 - PFLU_RS25125 0.25 -0.2 5,616,760 - PFLU_RS25125 0.25 -0.2 5,616,760 - PFLU_RS25125 0.25 -0.3 5,616,760 - PFLU_RS25125 0.25 +1.6 5,616,760 - PFLU_RS25125 0.25 +0.4 5,616,762 + PFLU_RS25125 0.25 -0.5 5,616,762 + PFLU_RS25125 0.25 +0.8 5,616,762 + PFLU_RS25125 0.25 -0.3 5,616,763 - PFLU_RS25125 0.25 +0.9 5,616,771 + PFLU_RS25125 0.26 -0.8 5,616,771 + PFLU_RS25125 0.26 +0.6 5,616,772 - PFLU_RS25125 0.27 +1.0 5,616,790 + PFLU_RS25125 0.29 -0.4 5,616,791 - PFLU_RS25125 0.30 -2.7 5,616,791 - PFLU_RS25125 0.30 -1.1 5,616,795 + PFLU_RS25125 0.30 -0.3 5,616,795 + PFLU_RS25125 0.30 +0.7 5,616,795 + PFLU_RS25125 0.30 -1.0 5,616,795 + PFLU_RS25125 0.30 +0.5 5,616,796 - PFLU_RS25125 0.30 +0.7 5,616,796 - PFLU_RS25125 0.30 -0.3 5,616,796 - PFLU_RS25125 0.30 +1.6 5,616,796 - PFLU_RS25125 0.30 +0.4 5,616,796 - PFLU_RS25125 0.30 +1.0 5,616,851 + PFLU_RS25125 0.39 +0.1 5,616,851 + PFLU_RS25125 0.39 +0.3 5,616,852 - PFLU_RS25125 0.40 +1.4 5,616,897 - PFLU_RS25125 0.47 +0.4 5,616,938 + PFLU_RS25125 0.53 -1.9 5,616,939 - PFLU_RS25125 0.54 -0.3 5,616,966 + PFLU_RS25125 0.58 +1.3 5,616,966 + PFLU_RS25125 0.58 +1.0 5,616,966 + PFLU_RS25125 0.58 -2.0 5,616,966 + PFLU_RS25125 0.58 -1.3 5,616,966 + PFLU_RS25125 0.58 +1.7 5,616,966 + PFLU_RS25125 0.58 -0.7 5,616,966 + PFLU_RS25125 0.58 -1.1 5,616,966 + PFLU_RS25125 0.58 -1.4 5,616,967 - PFLU_RS25125 0.58 -1.5 5,616,967 - PFLU_RS25125 0.58 -0.9 5,616,967 - PFLU_RS25125 0.58 +0.9 5,616,967 - PFLU_RS25125 0.58 -0.2 5,616,967 - PFLU_RS25125 0.58 -1.3 5,617,000 + PFLU_RS25125 0.64 -1.2 5,617,001 - PFLU_RS25125 0.64 -0.1 5,617,001 - PFLU_RS25125 0.64 -1.1 5,617,001 - PFLU_RS25125 0.64 +0.5 5,617,066 + PFLU_RS25125 0.74 -0.1 5,617,066 + PFLU_RS25125 0.74 -0.3 5,617,066 + PFLU_RS25125 0.74 -2.2 5,617,066 + PFLU_RS25125 0.74 +0.1 5,617,066 + PFLU_RS25125 0.74 +0.6 5,617,066 + PFLU_RS25125 0.74 +0.0 5,617,066 + PFLU_RS25125 0.74 +1.3 5,617,066 + PFLU_RS25125 0.74 -1.9 5,617,066 + PFLU_RS25125 0.74 +0.6 5,617,067 - PFLU_RS25125 0.74 -0.9 5,617,067 - PFLU_RS25125 0.74 -0.0 5,617,067 - PFLU_RS25125 0.74 -0.3 5,617,067 - PFLU_RS25125 0.74 -0.3 5,617,067 - PFLU_RS25125 0.74 -2.0 5,617,067 - PFLU_RS25125 0.74 -1.6 5,617,067 - PFLU_RS25125 0.74 -0.9 5,617,067 - PFLU_RS25125 0.74 -0.6 5,617,067 - PFLU_RS25125 0.74 -0.0 5,617,067 - PFLU_RS25125 0.74 -0.4 5,617,067 - PFLU_RS25125 0.74 +1.5 5,617,067 - PFLU_RS25125 0.74 +0.5 5,617,067 - PFLU_RS25125 0.74 +1.5 5,617,068 + PFLU_RS25125 0.75 +0.2 5,617,068 + PFLU_RS25125 0.75 -0.7 5,617,069 - PFLU_RS25125 0.75 -0.3 5,617,110 + PFLU_RS25125 0.81 -0.5 5,617,110 + PFLU_RS25125 0.81 +0.8 5,617,111 - PFLU_RS25125 0.82 -1.5 5,617,111 - PFLU_RS25125 0.82 -0.1 5,617,213 + +0.5 5,617,213 + -0.1 5,617,213 + +0.1 5,617,213 + -0.2 5,617,213 + -0.7 5,617,213 + +0.2 5,617,213 + +0.0 5,617,213 + +0.3 5,617,214 - -0.1 5,617,214 - -0.3 5,617,214 - -0.6 5,617,214 - -1.4 5,617,214 - -0.8 5,617,214 - -1.3 5,617,219 + -1.0 5,617,219 + -1.5 5,617,219 + -0.3 5,617,219 + +0.4 5,617,219 + -2.0 5,617,219 + +2.4 5,617,220 - +0.3
Or see this region's nucleotide sequence