Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS05590

Experiment: Growth with Chloride 200 mM; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS05580 and PFLU_RS05585 are separated by 228 nucleotidesPFLU_RS05585 and PFLU_RS05590 overlap by 8 nucleotidesPFLU_RS05590 and PFLU_RS05595 overlap by 4 nucleotides PFLU_RS05580: PFLU_RS05580 - MipA/OmpV family protein, at 1,258,645 to 1,259,415 _RS05580 PFLU_RS05585: PFLU_RS05585 - MtnX-like HAD-IB family phosphatase, at 1,259,644 to 1,260,348 _RS05585 PFLU_RS05590: PFLU_RS05590 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 1,260,341 to 1,261,735 _RS05590 PFLU_RS05595: PFLU_RS05595 - alpha/beta fold hydrolase, at 1,261,732 to 1,262,631 _RS05595 Position (kb) 1260 1261 1262Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1259.380 kb on + strandat 1259.380 kb on + strandat 1259.381 kb on - strandat 1259.381 kb on - strandat 1259.381 kb on - strandat 1259.437 kb on + strandat 1259.437 kb on + strandat 1259.437 kb on + strandat 1259.437 kb on + strandat 1259.437 kb on + strandat 1259.437 kb on + strandat 1259.438 kb on - strandat 1259.438 kb on - strandat 1259.438 kb on - strandat 1259.438 kb on - strandat 1259.438 kb on - strandat 1259.492 kb on + strandat 1259.492 kb on + strandat 1259.492 kb on + strandat 1259.501 kb on - strandat 1259.512 kb on - strandat 1259.518 kb on + strandat 1259.635 kb on + strandat 1259.635 kb on + strandat 1259.635 kb on + strandat 1259.635 kb on + strandat 1259.636 kb on - strandat 1259.636 kb on - strandat 1259.636 kb on - strandat 1259.636 kb on - strandat 1259.636 kb on - strandat 1259.659 kb on - strandat 1259.659 kb on - strandat 1259.659 kb on - strandat 1259.659 kb on - strandat 1259.842 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.971 kb on + strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1259.972 kb on - strand, within PFLU_RS05585at 1260.199 kb on + strand, within PFLU_RS05585at 1260.200 kb on - strand, within PFLU_RS05585at 1260.401 kb on + strandat 1260.401 kb on + strandat 1260.401 kb on + strandat 1260.401 kb on + strandat 1260.402 kb on - strandat 1260.402 kb on - strandat 1260.402 kb on - strandat 1260.402 kb on - strandat 1260.428 kb on + strandat 1260.428 kb on + strandat 1260.428 kb on + strandat 1260.428 kb on + strandat 1260.429 kb on - strandat 1260.429 kb on - strandat 1260.445 kb on + strandat 1260.446 kb on - strandat 1260.446 kb on - strandat 1260.446 kb on - strandat 1260.446 kb on - strandat 1260.446 kb on - strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.470 kb on + strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.471 kb on - strandat 1260.474 kb on + strandat 1260.474 kb on + strandat 1260.474 kb on + strandat 1260.474 kb on + strandat 1260.475 kb on - strandat 1260.475 kb on - strandat 1260.475 kb on - strandat 1260.475 kb on - strandat 1260.475 kb on - strandat 1260.498 kb on - strand, within PFLU_RS05590at 1260.498 kb on - strand, within PFLU_RS05590at 1260.608 kb on + strand, within PFLU_RS05590at 1260.608 kb on + strand, within PFLU_RS05590at 1260.608 kb on + strand, within PFLU_RS05590at 1260.800 kb on - strand, within PFLU_RS05590at 1260.929 kb on + strand, within PFLU_RS05590at 1260.929 kb on + strand, within PFLU_RS05590at 1260.930 kb on - strand, within PFLU_RS05590at 1260.930 kb on - strand, within PFLU_RS05590at 1260.930 kb on - strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1260.977 kb on + strand, within PFLU_RS05590at 1261.017 kb on - strand, within PFLU_RS05590at 1261.017 kb on - strand, within PFLU_RS05590at 1261.028 kb on + strand, within PFLU_RS05590at 1261.059 kb on - strand, within PFLU_RS05590at 1261.154 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.547 kb on + strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.548 kb on - strand, within PFLU_RS05590at 1261.581 kb on + strand, within PFLU_RS05590at 1261.581 kb on + strand, within PFLU_RS05590at 1261.581 kb on + strand, within PFLU_RS05590at 1261.581 kb on + strand, within PFLU_RS05590at 1261.582 kb on - strand, within PFLU_RS05590at 1261.626 kb on - strandat 1261.626 kb on - strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.628 kb on + strandat 1261.629 kb on - strandat 1261.629 kb on - strandat 1261.629 kb on - strandat 1261.629 kb on - strandat 1261.629 kb on - strandat 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.888 kb on + strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.889 kb on - strand, within PFLU_RS05595at 1261.896 kb on + strand, within PFLU_RS05595at 1261.957 kb on + strand, within PFLU_RS05595at 1262.050 kb on + strand, within PFLU_RS05595at 1262.051 kb on - strand, within PFLU_RS05595at 1262.051 kb on - strand, within PFLU_RS05595at 1262.051 kb on - strand, within PFLU_RS05595at 1262.051 kb on - strand, within PFLU_RS05595at 1262.095 kb on + strand, within PFLU_RS05595at 1262.095 kb on + strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.096 kb on - strand, within PFLU_RS05595at 1262.113 kb on + strand, within PFLU_RS05595at 1262.113 kb on + strand, within PFLU_RS05595at 1262.113 kb on + strand, within PFLU_RS05595at 1262.113 kb on + strand, within PFLU_RS05595at 1262.113 kb on + strand, within PFLU_RS05595at 1262.114 kb on - strand, within PFLU_RS05595at 1262.114 kb on - strand, within PFLU_RS05595at 1262.114 kb on - strand, within PFLU_RS05595at 1262.212 kb on + strand, within PFLU_RS05595at 1262.369 kb on + strand, within PFLU_RS05595at 1262.370 kb on - strand, within PFLU_RS05595at 1262.428 kb on + strand, within PFLU_RS05595at 1262.429 kb on - strand, within PFLU_RS05595at 1262.483 kb on - strand, within PFLU_RS05595at 1262.528 kb on + strand, within PFLU_RS05595at 1262.528 kb on + strand, within PFLU_RS05595at 1262.529 kb on - strand, within PFLU_RS05595at 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.559 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.563 kb on - strandat 1262.630 kb on - strandat 1262.630 kb on - strandat 1262.650 kb on - strandat 1262.720 kb on - strandat 1262.720 kb on - strandat 1262.720 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth with Chloride 200 mM; with MOPS
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1,259,380 + -0.4
1,259,380 + -1.9
1,259,381 - +0.4
1,259,381 - +0.5
1,259,381 - -3.3
1,259,437 + -0.2
1,259,437 + +1.4
1,259,437 + +0.8
1,259,437 + -0.7
1,259,437 + +0.8
1,259,437 + +0.7
1,259,438 - +0.6
1,259,438 - +1.5
1,259,438 - -1.5
1,259,438 - -2.1
1,259,438 - -2.9
1,259,492 + -2.0
1,259,492 + -1.0
1,259,492 + -0.9
1,259,501 - -1.2
1,259,512 - -1.1
1,259,518 + +0.4
1,259,635 + -0.3
1,259,635 + -1.2
1,259,635 + +0.4
1,259,635 + +0.8
1,259,636 - +1.4
1,259,636 - -3.0
1,259,636 - -1.2
1,259,636 - -0.4
1,259,636 - +1.7
1,259,659 - +1.2
1,259,659 - -2.1
1,259,659 - -0.9
1,259,659 - +1.6
1,259,842 + PFLU_RS05585 0.28 -1.7
1,259,971 + PFLU_RS05585 0.46 -1.0
1,259,971 + PFLU_RS05585 0.46 -0.4
1,259,971 + PFLU_RS05585 0.46 +1.6
1,259,971 + PFLU_RS05585 0.46 -0.4
1,259,971 + PFLU_RS05585 0.46 +1.0
1,259,971 + PFLU_RS05585 0.46 -2.1
1,259,971 + PFLU_RS05585 0.46 +2.2
1,259,971 + PFLU_RS05585 0.46 -1.0
1,259,971 + PFLU_RS05585 0.46 -1.6
1,259,972 - PFLU_RS05585 0.47 -0.0
1,259,972 - PFLU_RS05585 0.47 -1.1
1,259,972 - PFLU_RS05585 0.47 -2.3
1,259,972 - PFLU_RS05585 0.47 -1.7
1,259,972 - PFLU_RS05585 0.47 -2.6
1,259,972 - PFLU_RS05585 0.47 -1.3
1,259,972 - PFLU_RS05585 0.47 +2.9
1,259,972 - PFLU_RS05585 0.47 -2.2
1,259,972 - PFLU_RS05585 0.47 -0.9
1,259,972 - PFLU_RS05585 0.47 +1.7
1,259,972 - PFLU_RS05585 0.47 -0.1
1,259,972 - PFLU_RS05585 0.47 +1.2
1,260,199 + PFLU_RS05585 0.79 -1.0
1,260,200 - PFLU_RS05585 0.79 -0.2
1,260,401 + +1.3
1,260,401 + -0.4
1,260,401 + +1.2
1,260,401 + +0.6
1,260,402 - -1.2
1,260,402 - -0.3
1,260,402 - -0.4
1,260,402 - -0.0
1,260,428 + -1.0
1,260,428 + +1.3
1,260,428 + +0.7
1,260,428 + -0.0
1,260,429 - -2.7
1,260,429 - -1.6
1,260,445 + -1.8
1,260,446 - +0.3
1,260,446 - +0.6
1,260,446 - -0.4
1,260,446 - -2.3
1,260,446 - -0.1
1,260,470 + -1.2
1,260,470 + -1.9
1,260,470 + -0.0
1,260,470 + +0.3
1,260,470 + -1.1
1,260,470 + +0.3
1,260,470 + -1.0
1,260,471 - -0.3
1,260,471 - -0.7
1,260,471 - -3.3
1,260,471 - +0.1
1,260,471 - +0.5
1,260,471 - -1.0
1,260,471 - -1.9
1,260,471 - -2.8
1,260,471 - -1.0
1,260,471 - -1.7
1,260,471 - +0.8
1,260,471 - +2.6
1,260,471 - -0.9
1,260,471 - -3.2
1,260,474 + +0.3
1,260,474 + -0.9
1,260,474 + +0.5
1,260,474 + -0.2
1,260,475 - +0.3
1,260,475 - -1.0
1,260,475 - +0.6
1,260,475 - -1.5
1,260,475 - -0.7
1,260,498 - PFLU_RS05590 0.11 -2.7
1,260,498 - PFLU_RS05590 0.11 -2.0
1,260,608 + PFLU_RS05590 0.19 -0.9
1,260,608 + PFLU_RS05590 0.19 +1.1
1,260,608 + PFLU_RS05590 0.19 +1.8
1,260,800 - PFLU_RS05590 0.33 -2.0
1,260,929 + PFLU_RS05590 0.42 -0.5
1,260,929 + PFLU_RS05590 0.42 -2.7
1,260,930 - PFLU_RS05590 0.42 +0.5
1,260,930 - PFLU_RS05590 0.42 -2.3
1,260,930 - PFLU_RS05590 0.42 -0.8
1,260,977 + PFLU_RS05590 0.46 -0.5
1,260,977 + PFLU_RS05590 0.46 -2.8
1,260,977 + PFLU_RS05590 0.46 +0.4
1,260,977 + PFLU_RS05590 0.46 -1.4
1,260,977 + PFLU_RS05590 0.46 -1.4
1,260,977 + PFLU_RS05590 0.46 -2.6
1,260,977 + PFLU_RS05590 0.46 -2.4
1,260,977 + PFLU_RS05590 0.46 +1.0
1,261,017 - PFLU_RS05590 0.48 -0.2
1,261,017 - PFLU_RS05590 0.48 -0.1
1,261,028 + PFLU_RS05590 0.49 -0.5
1,261,059 - PFLU_RS05590 0.51 -0.4
1,261,154 + PFLU_RS05590 0.58 +1.2
1,261,547 + PFLU_RS05590 0.86 -0.7
1,261,547 + PFLU_RS05590 0.86 +2.1
1,261,547 + PFLU_RS05590 0.86 -1.6
1,261,547 + PFLU_RS05590 0.86 -0.0
1,261,547 + PFLU_RS05590 0.86 +1.6
1,261,547 + PFLU_RS05590 0.86 -1.0
1,261,547 + PFLU_RS05590 0.86 -2.7
1,261,547 + PFLU_RS05590 0.86 -0.2
1,261,547 + PFLU_RS05590 0.86 -0.4
1,261,547 + PFLU_RS05590 0.86 -2.6
1,261,547 + PFLU_RS05590 0.86 -0.7
1,261,547 + PFLU_RS05590 0.86 -0.7
1,261,547 + PFLU_RS05590 0.86 -0.7
1,261,547 + PFLU_RS05590 0.86 -0.7
1,261,547 + PFLU_RS05590 0.86 +0.4
1,261,548 - PFLU_RS05590 0.87 -2.0
1,261,548 - PFLU_RS05590 0.87 -0.4
1,261,548 - PFLU_RS05590 0.87 -2.3
1,261,548 - PFLU_RS05590 0.87 -0.9
1,261,548 - PFLU_RS05590 0.87 -0.5
1,261,548 - PFLU_RS05590 0.87 -2.5
1,261,548 - PFLU_RS05590 0.87 +0.6
1,261,581 + PFLU_RS05590 0.89 -0.6
1,261,581 + PFLU_RS05590 0.89 -0.7
1,261,581 + PFLU_RS05590 0.89 -0.2
1,261,581 + PFLU_RS05590 0.89 -0.2
1,261,582 - PFLU_RS05590 0.89 -0.9
1,261,626 - +0.3
1,261,626 - +1.9
1,261,628 + -1.2
1,261,628 + -1.6
1,261,628 + +0.8
1,261,628 + +1.3
1,261,628 + -0.7
1,261,628 + -1.9
1,261,628 + -1.7
1,261,629 - -1.3
1,261,629 - +0.7
1,261,629 - -1.7
1,261,629 - +0.1
1,261,629 - -1.6
1,261,888 + PFLU_RS05595 0.17 +0.1
1,261,888 + PFLU_RS05595 0.17 -1.4
1,261,888 + PFLU_RS05595 0.17 +1.6
1,261,888 + PFLU_RS05595 0.17 -0.5
1,261,888 + PFLU_RS05595 0.17 +2.4
1,261,888 + PFLU_RS05595 0.17 +0.4
1,261,888 + PFLU_RS05595 0.17 +1.0
1,261,888 + PFLU_RS05595 0.17 -0.3
1,261,888 + PFLU_RS05595 0.17 -1.0
1,261,888 + PFLU_RS05595 0.17 +0.9
1,261,889 - PFLU_RS05595 0.17 +0.4
1,261,889 - PFLU_RS05595 0.17 +0.5
1,261,889 - PFLU_RS05595 0.17 +0.9
1,261,889 - PFLU_RS05595 0.17 -1.4
1,261,889 - PFLU_RS05595 0.17 +1.2
1,261,889 - PFLU_RS05595 0.17 +1.6
1,261,889 - PFLU_RS05595 0.17 -0.0
1,261,889 - PFLU_RS05595 0.17 -1.7
1,261,889 - PFLU_RS05595 0.17 +0.7
1,261,889 - PFLU_RS05595 0.17 -1.7
1,261,896 + PFLU_RS05595 0.18 +0.3
1,261,957 + PFLU_RS05595 0.25 -0.1
1,262,050 + PFLU_RS05595 0.35 -0.4
1,262,051 - PFLU_RS05595 0.35 +0.8
1,262,051 - PFLU_RS05595 0.35 -1.6
1,262,051 - PFLU_RS05595 0.35 +1.0
1,262,051 - PFLU_RS05595 0.35 -1.2
1,262,095 + PFLU_RS05595 0.40 +2.3
1,262,095 + PFLU_RS05595 0.40 +1.3
1,262,096 - PFLU_RS05595 0.40 -0.5
1,262,096 - PFLU_RS05595 0.40 -3.1
1,262,096 - PFLU_RS05595 0.40 -2.2
1,262,096 - PFLU_RS05595 0.40 +0.5
1,262,096 - PFLU_RS05595 0.40 +2.3
1,262,096 - PFLU_RS05595 0.40 -2.5
1,262,096 - PFLU_RS05595 0.40 -1.9
1,262,113 + PFLU_RS05595 0.42 -0.9
1,262,113 + PFLU_RS05595 0.42 -0.9
1,262,113 + PFLU_RS05595 0.42 -1.1
1,262,113 + PFLU_RS05595 0.42 +2.6
1,262,113 + PFLU_RS05595 0.42 +0.8
1,262,114 - PFLU_RS05595 0.42 +0.4
1,262,114 - PFLU_RS05595 0.42 -1.0
1,262,114 - PFLU_RS05595 0.42 -1.1
1,262,212 + PFLU_RS05595 0.53 -0.1
1,262,369 + PFLU_RS05595 0.71 -1.8
1,262,370 - PFLU_RS05595 0.71 -0.4
1,262,428 + PFLU_RS05595 0.77 -0.0
1,262,429 - PFLU_RS05595 0.77 +0.3
1,262,483 - PFLU_RS05595 0.83 -0.9
1,262,528 + PFLU_RS05595 0.88 -0.0
1,262,528 + PFLU_RS05595 0.88 -2.9
1,262,529 - PFLU_RS05595 0.89 -2.4
1,262,559 - +0.5
1,262,559 - -0.6
1,262,559 - -2.3
1,262,559 - +1.2
1,262,559 - +1.5
1,262,559 - -0.5
1,262,559 - -0.5
1,262,563 - -1.6
1,262,563 - -1.6
1,262,563 - -0.1
1,262,563 - +0.1
1,262,563 - +0.6
1,262,563 - -1.2
1,262,563 - -1.6
1,262,563 - +1.8
1,262,630 - +1.0
1,262,630 - +3.2
1,262,650 - +0.8
1,262,720 - +0.4
1,262,720 - -2.6
1,262,720 - -2.2

Or see this region's nucleotide sequence