Experiment: Growth with Chloride 200 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS05580 and PFLU_RS05585 are separated by 228 nucleotides PFLU_RS05585 and PFLU_RS05590 overlap by 8 nucleotides PFLU_RS05590 and PFLU_RS05595 overlap by 4 nucleotides
PFLU_RS05580: PFLU_RS05580 - MipA/OmpV family protein, at 1,258,645 to 1,259,415
_RS05580
PFLU_RS05585: PFLU_RS05585 - MtnX-like HAD-IB family phosphatase, at 1,259,644 to 1,260,348
_RS05585
PFLU_RS05590: PFLU_RS05590 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 1,260,341 to 1,261,735
_RS05590
PFLU_RS05595: PFLU_RS05595 - alpha/beta fold hydrolase, at 1,261,732 to 1,262,631
_RS05595
Position (kb)
1260
1261
1262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1259.380 kb on + strand at 1259.380 kb on + strand at 1259.381 kb on - strand at 1259.381 kb on - strand at 1259.381 kb on - strand at 1259.437 kb on + strand at 1259.437 kb on + strand at 1259.437 kb on + strand at 1259.437 kb on + strand at 1259.437 kb on + strand at 1259.437 kb on + strand at 1259.438 kb on - strand at 1259.438 kb on - strand at 1259.438 kb on - strand at 1259.438 kb on - strand at 1259.438 kb on - strand at 1259.492 kb on + strand at 1259.492 kb on + strand at 1259.492 kb on + strand at 1259.501 kb on - strand at 1259.512 kb on - strand at 1259.518 kb on + strand at 1259.635 kb on + strand at 1259.635 kb on + strand at 1259.635 kb on + strand at 1259.635 kb on + strand at 1259.636 kb on - strand at 1259.636 kb on - strand at 1259.636 kb on - strand at 1259.636 kb on - strand at 1259.636 kb on - strand at 1259.659 kb on - strand at 1259.659 kb on - strand at 1259.659 kb on - strand at 1259.659 kb on - strand at 1259.842 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.971 kb on + strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1259.972 kb on - strand, within PFLU_RS05585 at 1260.199 kb on + strand, within PFLU_RS05585 at 1260.200 kb on - strand, within PFLU_RS05585 at 1260.401 kb on + strand at 1260.401 kb on + strand at 1260.401 kb on + strand at 1260.401 kb on + strand at 1260.402 kb on - strand at 1260.402 kb on - strand at 1260.402 kb on - strand at 1260.402 kb on - strand at 1260.428 kb on + strand at 1260.428 kb on + strand at 1260.428 kb on + strand at 1260.428 kb on + strand at 1260.429 kb on - strand at 1260.429 kb on - strand at 1260.445 kb on + strand at 1260.446 kb on - strand at 1260.446 kb on - strand at 1260.446 kb on - strand at 1260.446 kb on - strand at 1260.446 kb on - strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.470 kb on + strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.471 kb on - strand at 1260.474 kb on + strand at 1260.474 kb on + strand at 1260.474 kb on + strand at 1260.474 kb on + strand at 1260.475 kb on - strand at 1260.475 kb on - strand at 1260.475 kb on - strand at 1260.475 kb on - strand at 1260.475 kb on - strand at 1260.498 kb on - strand, within PFLU_RS05590 at 1260.498 kb on - strand, within PFLU_RS05590 at 1260.608 kb on + strand, within PFLU_RS05590 at 1260.608 kb on + strand, within PFLU_RS05590 at 1260.608 kb on + strand, within PFLU_RS05590 at 1260.800 kb on - strand, within PFLU_RS05590 at 1260.929 kb on + strand, within PFLU_RS05590 at 1260.929 kb on + strand, within PFLU_RS05590 at 1260.930 kb on - strand, within PFLU_RS05590 at 1260.930 kb on - strand, within PFLU_RS05590 at 1260.930 kb on - strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1260.977 kb on + strand, within PFLU_RS05590 at 1261.017 kb on - strand, within PFLU_RS05590 at 1261.017 kb on - strand, within PFLU_RS05590 at 1261.028 kb on + strand, within PFLU_RS05590 at 1261.059 kb on - strand, within PFLU_RS05590 at 1261.154 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.547 kb on + strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.548 kb on - strand, within PFLU_RS05590 at 1261.581 kb on + strand, within PFLU_RS05590 at 1261.581 kb on + strand, within PFLU_RS05590 at 1261.581 kb on + strand, within PFLU_RS05590 at 1261.581 kb on + strand, within PFLU_RS05590 at 1261.582 kb on - strand, within PFLU_RS05590 at 1261.626 kb on - strand at 1261.626 kb on - strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.628 kb on + strand at 1261.629 kb on - strand at 1261.629 kb on - strand at 1261.629 kb on - strand at 1261.629 kb on - strand at 1261.629 kb on - strand at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.888 kb on + strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.889 kb on - strand, within PFLU_RS05595 at 1261.896 kb on + strand, within PFLU_RS05595 at 1261.957 kb on + strand, within PFLU_RS05595 at 1262.050 kb on + strand, within PFLU_RS05595 at 1262.051 kb on - strand, within PFLU_RS05595 at 1262.051 kb on - strand, within PFLU_RS05595 at 1262.051 kb on - strand, within PFLU_RS05595 at 1262.051 kb on - strand, within PFLU_RS05595 at 1262.095 kb on + strand, within PFLU_RS05595 at 1262.095 kb on + strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.096 kb on - strand, within PFLU_RS05595 at 1262.113 kb on + strand, within PFLU_RS05595 at 1262.113 kb on + strand, within PFLU_RS05595 at 1262.113 kb on + strand, within PFLU_RS05595 at 1262.113 kb on + strand, within PFLU_RS05595 at 1262.113 kb on + strand, within PFLU_RS05595 at 1262.114 kb on - strand, within PFLU_RS05595 at 1262.114 kb on - strand, within PFLU_RS05595 at 1262.114 kb on - strand, within PFLU_RS05595 at 1262.212 kb on + strand, within PFLU_RS05595 at 1262.369 kb on + strand, within PFLU_RS05595 at 1262.370 kb on - strand, within PFLU_RS05595 at 1262.428 kb on + strand, within PFLU_RS05595 at 1262.429 kb on - strand, within PFLU_RS05595 at 1262.483 kb on - strand, within PFLU_RS05595 at 1262.528 kb on + strand, within PFLU_RS05595 at 1262.528 kb on + strand, within PFLU_RS05595 at 1262.529 kb on - strand, within PFLU_RS05595 at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.630 kb on - strand at 1262.630 kb on - strand at 1262.650 kb on - strand at 1262.720 kb on - strand at 1262.720 kb on - strand at 1262.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 200 mM; with MOPS remove 1,259,380 + -0.4 1,259,380 + -1.9 1,259,381 - +0.4 1,259,381 - +0.5 1,259,381 - -3.3 1,259,437 + -0.2 1,259,437 + +1.4 1,259,437 + +0.8 1,259,437 + -0.7 1,259,437 + +0.8 1,259,437 + +0.7 1,259,438 - +0.6 1,259,438 - +1.5 1,259,438 - -1.5 1,259,438 - -2.1 1,259,438 - -2.9 1,259,492 + -2.0 1,259,492 + -1.0 1,259,492 + -0.9 1,259,501 - -1.2 1,259,512 - -1.1 1,259,518 + +0.4 1,259,635 + -0.3 1,259,635 + -1.2 1,259,635 + +0.4 1,259,635 + +0.8 1,259,636 - +1.4 1,259,636 - -3.0 1,259,636 - -1.2 1,259,636 - -0.4 1,259,636 - +1.7 1,259,659 - +1.2 1,259,659 - -2.1 1,259,659 - -0.9 1,259,659 - +1.6 1,259,842 + PFLU_RS05585 0.28 -1.7 1,259,971 + PFLU_RS05585 0.46 -1.0 1,259,971 + PFLU_RS05585 0.46 -0.4 1,259,971 + PFLU_RS05585 0.46 +1.6 1,259,971 + PFLU_RS05585 0.46 -0.4 1,259,971 + PFLU_RS05585 0.46 +1.0 1,259,971 + PFLU_RS05585 0.46 -2.1 1,259,971 + PFLU_RS05585 0.46 +2.2 1,259,971 + PFLU_RS05585 0.46 -1.0 1,259,971 + PFLU_RS05585 0.46 -1.6 1,259,972 - PFLU_RS05585 0.47 -0.0 1,259,972 - PFLU_RS05585 0.47 -1.1 1,259,972 - PFLU_RS05585 0.47 -2.3 1,259,972 - PFLU_RS05585 0.47 -1.7 1,259,972 - PFLU_RS05585 0.47 -2.6 1,259,972 - PFLU_RS05585 0.47 -1.3 1,259,972 - PFLU_RS05585 0.47 +2.9 1,259,972 - PFLU_RS05585 0.47 -2.2 1,259,972 - PFLU_RS05585 0.47 -0.9 1,259,972 - PFLU_RS05585 0.47 +1.7 1,259,972 - PFLU_RS05585 0.47 -0.1 1,259,972 - PFLU_RS05585 0.47 +1.2 1,260,199 + PFLU_RS05585 0.79 -1.0 1,260,200 - PFLU_RS05585 0.79 -0.2 1,260,401 + +1.3 1,260,401 + -0.4 1,260,401 + +1.2 1,260,401 + +0.6 1,260,402 - -1.2 1,260,402 - -0.3 1,260,402 - -0.4 1,260,402 - -0.0 1,260,428 + -1.0 1,260,428 + +1.3 1,260,428 + +0.7 1,260,428 + -0.0 1,260,429 - -2.7 1,260,429 - -1.6 1,260,445 + -1.8 1,260,446 - +0.3 1,260,446 - +0.6 1,260,446 - -0.4 1,260,446 - -2.3 1,260,446 - -0.1 1,260,470 + -1.2 1,260,470 + -1.9 1,260,470 + -0.0 1,260,470 + +0.3 1,260,470 + -1.1 1,260,470 + +0.3 1,260,470 + -1.0 1,260,471 - -0.3 1,260,471 - -0.7 1,260,471 - -3.3 1,260,471 - +0.1 1,260,471 - +0.5 1,260,471 - -1.0 1,260,471 - -1.9 1,260,471 - -2.8 1,260,471 - -1.0 1,260,471 - -1.7 1,260,471 - +0.8 1,260,471 - +2.6 1,260,471 - -0.9 1,260,471 - -3.2 1,260,474 + +0.3 1,260,474 + -0.9 1,260,474 + +0.5 1,260,474 + -0.2 1,260,475 - +0.3 1,260,475 - -1.0 1,260,475 - +0.6 1,260,475 - -1.5 1,260,475 - -0.7 1,260,498 - PFLU_RS05590 0.11 -2.7 1,260,498 - PFLU_RS05590 0.11 -2.0 1,260,608 + PFLU_RS05590 0.19 -0.9 1,260,608 + PFLU_RS05590 0.19 +1.1 1,260,608 + PFLU_RS05590 0.19 +1.8 1,260,800 - PFLU_RS05590 0.33 -2.0 1,260,929 + PFLU_RS05590 0.42 -0.5 1,260,929 + PFLU_RS05590 0.42 -2.7 1,260,930 - PFLU_RS05590 0.42 +0.5 1,260,930 - PFLU_RS05590 0.42 -2.3 1,260,930 - PFLU_RS05590 0.42 -0.8 1,260,977 + PFLU_RS05590 0.46 -0.5 1,260,977 + PFLU_RS05590 0.46 -2.8 1,260,977 + PFLU_RS05590 0.46 +0.4 1,260,977 + PFLU_RS05590 0.46 -1.4 1,260,977 + PFLU_RS05590 0.46 -1.4 1,260,977 + PFLU_RS05590 0.46 -2.6 1,260,977 + PFLU_RS05590 0.46 -2.4 1,260,977 + PFLU_RS05590 0.46 +1.0 1,261,017 - PFLU_RS05590 0.48 -0.2 1,261,017 - PFLU_RS05590 0.48 -0.1 1,261,028 + PFLU_RS05590 0.49 -0.5 1,261,059 - PFLU_RS05590 0.51 -0.4 1,261,154 + PFLU_RS05590 0.58 +1.2 1,261,547 + PFLU_RS05590 0.86 -0.7 1,261,547 + PFLU_RS05590 0.86 +2.1 1,261,547 + PFLU_RS05590 0.86 -1.6 1,261,547 + PFLU_RS05590 0.86 -0.0 1,261,547 + PFLU_RS05590 0.86 +1.6 1,261,547 + PFLU_RS05590 0.86 -1.0 1,261,547 + PFLU_RS05590 0.86 -2.7 1,261,547 + PFLU_RS05590 0.86 -0.2 1,261,547 + PFLU_RS05590 0.86 -0.4 1,261,547 + PFLU_RS05590 0.86 -2.6 1,261,547 + PFLU_RS05590 0.86 -0.7 1,261,547 + PFLU_RS05590 0.86 -0.7 1,261,547 + PFLU_RS05590 0.86 -0.7 1,261,547 + PFLU_RS05590 0.86 -0.7 1,261,547 + PFLU_RS05590 0.86 +0.4 1,261,548 - PFLU_RS05590 0.87 -2.0 1,261,548 - PFLU_RS05590 0.87 -0.4 1,261,548 - PFLU_RS05590 0.87 -2.3 1,261,548 - PFLU_RS05590 0.87 -0.9 1,261,548 - PFLU_RS05590 0.87 -0.5 1,261,548 - PFLU_RS05590 0.87 -2.5 1,261,548 - PFLU_RS05590 0.87 +0.6 1,261,581 + PFLU_RS05590 0.89 -0.6 1,261,581 + PFLU_RS05590 0.89 -0.7 1,261,581 + PFLU_RS05590 0.89 -0.2 1,261,581 + PFLU_RS05590 0.89 -0.2 1,261,582 - PFLU_RS05590 0.89 -0.9 1,261,626 - +0.3 1,261,626 - +1.9 1,261,628 + -1.2 1,261,628 + -1.6 1,261,628 + +0.8 1,261,628 + +1.3 1,261,628 + -0.7 1,261,628 + -1.9 1,261,628 + -1.7 1,261,629 - -1.3 1,261,629 - +0.7 1,261,629 - -1.7 1,261,629 - +0.1 1,261,629 - -1.6 1,261,888 + PFLU_RS05595 0.17 +0.1 1,261,888 + PFLU_RS05595 0.17 -1.4 1,261,888 + PFLU_RS05595 0.17 +1.6 1,261,888 + PFLU_RS05595 0.17 -0.5 1,261,888 + PFLU_RS05595 0.17 +2.4 1,261,888 + PFLU_RS05595 0.17 +0.4 1,261,888 + PFLU_RS05595 0.17 +1.0 1,261,888 + PFLU_RS05595 0.17 -0.3 1,261,888 + PFLU_RS05595 0.17 -1.0 1,261,888 + PFLU_RS05595 0.17 +0.9 1,261,889 - PFLU_RS05595 0.17 +0.4 1,261,889 - PFLU_RS05595 0.17 +0.5 1,261,889 - PFLU_RS05595 0.17 +0.9 1,261,889 - PFLU_RS05595 0.17 -1.4 1,261,889 - PFLU_RS05595 0.17 +1.2 1,261,889 - PFLU_RS05595 0.17 +1.6 1,261,889 - PFLU_RS05595 0.17 -0.0 1,261,889 - PFLU_RS05595 0.17 -1.7 1,261,889 - PFLU_RS05595 0.17 +0.7 1,261,889 - PFLU_RS05595 0.17 -1.7 1,261,896 + PFLU_RS05595 0.18 +0.3 1,261,957 + PFLU_RS05595 0.25 -0.1 1,262,050 + PFLU_RS05595 0.35 -0.4 1,262,051 - PFLU_RS05595 0.35 +0.8 1,262,051 - PFLU_RS05595 0.35 -1.6 1,262,051 - PFLU_RS05595 0.35 +1.0 1,262,051 - PFLU_RS05595 0.35 -1.2 1,262,095 + PFLU_RS05595 0.40 +2.3 1,262,095 + PFLU_RS05595 0.40 +1.3 1,262,096 - PFLU_RS05595 0.40 -0.5 1,262,096 - PFLU_RS05595 0.40 -3.1 1,262,096 - PFLU_RS05595 0.40 -2.2 1,262,096 - PFLU_RS05595 0.40 +0.5 1,262,096 - PFLU_RS05595 0.40 +2.3 1,262,096 - PFLU_RS05595 0.40 -2.5 1,262,096 - PFLU_RS05595 0.40 -1.9 1,262,113 + PFLU_RS05595 0.42 -0.9 1,262,113 + PFLU_RS05595 0.42 -0.9 1,262,113 + PFLU_RS05595 0.42 -1.1 1,262,113 + PFLU_RS05595 0.42 +2.6 1,262,113 + PFLU_RS05595 0.42 +0.8 1,262,114 - PFLU_RS05595 0.42 +0.4 1,262,114 - PFLU_RS05595 0.42 -1.0 1,262,114 - PFLU_RS05595 0.42 -1.1 1,262,212 + PFLU_RS05595 0.53 -0.1 1,262,369 + PFLU_RS05595 0.71 -1.8 1,262,370 - PFLU_RS05595 0.71 -0.4 1,262,428 + PFLU_RS05595 0.77 -0.0 1,262,429 - PFLU_RS05595 0.77 +0.3 1,262,483 - PFLU_RS05595 0.83 -0.9 1,262,528 + PFLU_RS05595 0.88 -0.0 1,262,528 + PFLU_RS05595 0.88 -2.9 1,262,529 - PFLU_RS05595 0.89 -2.4 1,262,559 - +0.5 1,262,559 - -0.6 1,262,559 - -2.3 1,262,559 - +1.2 1,262,559 - +1.5 1,262,559 - -0.5 1,262,559 - -0.5 1,262,563 - -1.6 1,262,563 - -1.6 1,262,563 - -0.1 1,262,563 - +0.1 1,262,563 - +0.6 1,262,563 - -1.2 1,262,563 - -1.6 1,262,563 - +1.8 1,262,630 - +1.0 1,262,630 - +3.2 1,262,650 - +0.8 1,262,720 - +0.4 1,262,720 - -2.6 1,262,720 - -2.2
Or see this region's nucleotide sequence