Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0201 and Dshi_0202 are separated by 3 nucleotides Dshi_0202 and Dshi_0203 are separated by 68 nucleotides Dshi_0203 and Dshi_0204 are separated by 61 nucleotides
Dshi_0201: Dshi_0201 - GTP-binding protein Era (RefSeq), at 199,783 to 200,709
_0201
Dshi_0202: Dshi_0202 - protein of unknown function DUF1491 (RefSeq), at 200,713 to 201,045
_0202
Dshi_0203: Dshi_0203 - DNA repair protein RecO (RefSeq), at 201,114 to 201,839
_0203
Dshi_0204: Dshi_0204 - acyl-CoA dehydrogenase domain protein (RefSeq), at 201,901 to 203,595
_0204
Position (kb)
201
202 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 200.654 kb on + strand at 200.786 kb on + strand, within Dshi_0202 at 200.786 kb on + strand, within Dshi_0202 at 200.992 kb on + strand, within Dshi_0202 at 201.059 kb on - strand at 201.132 kb on - strand at 201.194 kb on - strand, within Dshi_0203 at 201.715 kb on + strand, within Dshi_0203 at 201.875 kb on + strand at 202.055 kb on + strand at 202.055 kb on + strand at 202.123 kb on - strand, within Dshi_0204 at 202.301 kb on - strand, within Dshi_0204 at 202.312 kb on - strand, within Dshi_0204 at 202.382 kb on + strand, within Dshi_0204 at 202.467 kb on - strand, within Dshi_0204 at 202.527 kb on + strand, within Dshi_0204 at 202.550 kb on + strand, within Dshi_0204 at 202.577 kb on - strand, within Dshi_0204 at 202.590 kb on - strand, within Dshi_0204 at 202.642 kb on + strand, within Dshi_0204 at 202.657 kb on + strand, within Dshi_0204 at 202.685 kb on + strand, within Dshi_0204
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 200,654 + -0.6 200,786 + Dshi_0202 0.22 -1.0 200,786 + Dshi_0202 0.22 +0.3 200,992 + Dshi_0202 0.84 -2.6 201,059 - -1.3 201,132 - -3.7 201,194 - Dshi_0203 0.11 -3.5 201,715 + Dshi_0203 0.83 -4.0 201,875 + -0.9 202,055 + +0.7 202,055 + +0.9 202,123 - Dshi_0204 0.13 +1.5 202,301 - Dshi_0204 0.24 +0.3 202,312 - Dshi_0204 0.24 -0.2 202,382 + Dshi_0204 0.28 +0.1 202,467 - Dshi_0204 0.33 +0.3 202,527 + Dshi_0204 0.37 +0.1 202,550 + Dshi_0204 0.38 +1.0 202,577 - Dshi_0204 0.40 +0.7 202,590 - Dshi_0204 0.41 +0.5 202,642 + Dshi_0204 0.44 +0.9 202,657 + Dshi_0204 0.45 +0.5 202,685 + Dshi_0204 0.46 +1.0
Or see this region's nucleotide sequence