Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0064 and Dshi_0065 are separated by 77 nucleotides Dshi_0065 and Dshi_0066 are separated by 115 nucleotides Dshi_0066 and Dshi_0067 overlap by 4 nucleotides
Dshi_0064: Dshi_0064 - peptidase U62 modulator of DNA gyrase (RefSeq), at 64,554 to 65,900
_0064
Dshi_0065: Dshi_0065 - hypothetical protein (RefSeq), at 65,978 to 67,468
_0065
Dshi_0066: Dshi_0066 - short-chain dehydrogenase/reductase SDR (RefSeq), at 67,584 to 68,342
_0066
Dshi_0067: Dshi_0067 - Enoyl-CoA hydratase/isomerase (RefSeq), at 68,339 to 69,085
_0067
Position (kb)
65
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1 at 65.082 kb on - strand, within Dshi_0064 at 65.240 kb on + strand, within Dshi_0064 at 65.319 kb on + strand, within Dshi_0064 at 65.486 kb on - strand, within Dshi_0064 at 65.696 kb on - strand, within Dshi_0064 at 65.865 kb on - strand at 65.882 kb on - strand at 65.939 kb on - strand at 66.085 kb on - strand at 66.096 kb on + strand at 66.132 kb on + strand, within Dshi_0065 at 66.318 kb on + strand, within Dshi_0065 at 66.475 kb on - strand, within Dshi_0065 at 66.673 kb on - strand, within Dshi_0065 at 66.817 kb on - strand, within Dshi_0065 at 66.990 kb on - strand, within Dshi_0065 at 67.067 kb on + strand, within Dshi_0065 at 67.077 kb on + strand, within Dshi_0065 at 67.080 kb on - strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.126 kb on + strand at 67.135 kb on - strand, within Dshi_0065 at 67.242 kb on - strand, within Dshi_0065 at 67.365 kb on + strand at 67.366 kb on + strand at 67.373 kb on - strand at 67.395 kb on - strand at 67.406 kb on - strand at 67.417 kb on - strand at 67.456 kb on + strand at 67.474 kb on - strand at 67.527 kb on - strand at 67.531 kb on + strand at 67.531 kb on + strand at 67.537 kb on - strand at 67.553 kb on - strand at 67.563 kb on + strand at 67.578 kb on - strand at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.810 kb on - strand at 67.810 kb on - strand, within Dshi_0066 at 67.851 kb on - strand, within Dshi_0066 at 67.920 kb on - strand, within Dshi_0066 at 67.931 kb on + strand, within Dshi_0066 at 68.208 kb on + strand, within Dshi_0066 at 68.251 kb on - strand, within Dshi_0066 at 68.393 kb on + strand at 68.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 65,082 - Dshi_0064 0.39 -0.7 65,240 + Dshi_0064 0.51 -0.6 65,319 + Dshi_0064 0.57 -2.6 65,486 - Dshi_0064 0.69 +0.5 65,696 - Dshi_0064 0.85 -0.5 65,865 - -0.1 65,882 - -0.1 65,939 - +0.1 66,085 - +0.3 66,096 + +0.0 66,132 + Dshi_0065 0.10 -0.6 66,318 + Dshi_0065 0.23 -0.2 66,475 - Dshi_0065 0.33 +1.1 66,673 - Dshi_0065 0.47 -0.1 66,817 - Dshi_0065 0.56 +0.2 66,990 - Dshi_0065 0.68 +0.2 67,067 + Dshi_0065 0.73 +0.1 67,077 + Dshi_0065 0.74 -0.7 67,080 - Dshi_0065 0.74 -1.6 67,104 + Dshi_0065 0.76 +0.7 67,104 + Dshi_0065 0.76 -1.4 67,126 + -1.6 67,135 - Dshi_0065 0.78 -1.1 67,242 - Dshi_0065 0.85 -0.8 67,365 + +0.1 67,366 + -0.3 67,373 - -0.6 67,395 - -0.4 67,406 - +0.5 67,417 - -0.2 67,456 + +0.1 67,474 - -1.1 67,527 - -0.6 67,531 + +0.9 67,531 + +0.5 67,537 - -0.3 67,553 - +0.7 67,563 + +0.2 67,578 - +0.4 67,748 + Dshi_0066 0.22 -0.3 67,748 + Dshi_0066 0.22 +0.2 67,748 + Dshi_0066 0.22 +0.1 67,810 - -0.1 67,810 - Dshi_0066 0.30 +0.1 67,851 - Dshi_0066 0.35 -0.6 67,920 - Dshi_0066 0.44 -0.4 67,931 + Dshi_0066 0.46 -1.2 68,208 + Dshi_0066 0.82 -0.9 68,251 - Dshi_0066 0.88 -0.5 68,393 + +0.6 68,398 + -0.0
Or see this region's nucleotide sequence