Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2213 and Rru_A2214 are separated by 69 nucleotides Rru_A2214 and Rru_A2215 overlap by 4 nucleotides Rru_A2215 and Rru_A2216 are separated by 145 nucleotides Rru_A2216 and Rru_A2217 are separated by 129 nucleotides
Rru_A2213: Rru_A2213 - sulfotransferase (NCBI), at 2,571,302 to 2,573,272
_A2213
Rru_A2214: Rru_A2214 - Transcriptional Regulator, LysR family (NCBI), at 2,573,342 to 2,574,268
_A2214
Rru_A2215: Rru_A2215 - Transcriptional Regulator, LysR family (NCBI), at 2,574,265 to 2,575,218
_A2215
Rru_A2216: Rru_A2216 - Protein of unknown function DUF1127 (NCBI), at 2,575,364 to 2,575,714
_A2216
Rru_A2217: Rru_A2217 - hypothetical protein (NCBI), at 2,575,844 to 2,576,596
_A2217
Position (kb)
2574
2575
2576 Strain fitness (log2 ratio)
-2
-1
0
1 at 2573.286 kb on + strand at 2573.286 kb on + strand at 2573.287 kb on - strand at 2573.448 kb on + strand, within Rru_A2214 at 2573.449 kb on - strand, within Rru_A2214 at 2573.655 kb on + strand, within Rru_A2214 at 2573.655 kb on + strand, within Rru_A2214 at 2573.656 kb on - strand, within Rru_A2214 at 2573.656 kb on - strand, within Rru_A2214 at 2573.814 kb on + strand, within Rru_A2214 at 2574.063 kb on + strand, within Rru_A2214 at 2574.064 kb on - strand, within Rru_A2214 at 2574.064 kb on - strand, within Rru_A2214 at 2574.064 kb on - strand, within Rru_A2214 at 2574.402 kb on - strand, within Rru_A2215 at 2574.402 kb on - strand, within Rru_A2215 at 2574.541 kb on - strand, within Rru_A2215 at 2574.541 kb on - strand, within Rru_A2215 at 2574.764 kb on + strand, within Rru_A2215 at 2574.765 kb on - strand, within Rru_A2215 at 2574.765 kb on - strand, within Rru_A2215 at 2574.811 kb on - strand, within Rru_A2215 at 2575.013 kb on + strand, within Rru_A2215 at 2575.013 kb on + strand, within Rru_A2215 at 2575.013 kb on + strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.150 kb on - strand at 2575.308 kb on - strand at 2575.308 kb on - strand at 2575.308 kb on - strand at 2575.442 kb on + strand, within Rru_A2216 at 2575.562 kb on + strand, within Rru_A2216 at 2575.563 kb on - strand, within Rru_A2216 at 2575.563 kb on - strand, within Rru_A2216 at 2575.812 kb on + strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 2,573,286 + +0.1 2,573,286 + +0.1 2,573,287 - -0.9 2,573,448 + Rru_A2214 0.11 +0.1 2,573,449 - Rru_A2214 0.12 -1.8 2,573,655 + Rru_A2214 0.34 +0.1 2,573,655 + Rru_A2214 0.34 -0.9 2,573,656 - Rru_A2214 0.34 +0.1 2,573,656 - Rru_A2214 0.34 +0.1 2,573,814 + Rru_A2214 0.51 +0.1 2,574,063 + Rru_A2214 0.78 +0.1 2,574,064 - Rru_A2214 0.78 +0.1 2,574,064 - Rru_A2214 0.78 +0.1 2,574,064 - Rru_A2214 0.78 -0.8 2,574,402 - Rru_A2215 0.14 -0.9 2,574,402 - Rru_A2215 0.14 +0.1 2,574,541 - Rru_A2215 0.29 +0.1 2,574,541 - Rru_A2215 0.29 -1.0 2,574,764 + Rru_A2215 0.52 -0.6 2,574,765 - Rru_A2215 0.52 +0.1 2,574,765 - Rru_A2215 0.52 +0.1 2,574,811 - Rru_A2215 0.57 +0.1 2,575,013 + Rru_A2215 0.78 +0.1 2,575,013 + Rru_A2215 0.78 +0.1 2,575,013 + Rru_A2215 0.78 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,150 - -1.1 2,575,308 - -1.1 2,575,308 - -2.2 2,575,308 - +0.1 2,575,442 + Rru_A2216 0.22 -1.6 2,575,562 + Rru_A2216 0.56 +0.1 2,575,563 - Rru_A2216 0.57 +0.1 2,575,563 - Rru_A2216 0.57 +0.1 2,575,812 + -1.4 2,575,813 - +0.1 2,575,813 - +0.1 2,575,813 - -1.3 2,575,813 - +0.1 2,575,813 - -1.0
Or see this region's nucleotide sequence