Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1309 and Rru_A1310 are separated by 13 nucleotides Rru_A1310 and Rru_A1311 are separated by 52 nucleotides Rru_A1311 and Rru_A1312 are separated by 130 nucleotides
Rru_A1309: Rru_A1309 - acetoacetyl-CoA reductase (NCBI), at 1,544,051 to 1,546,375
_A1309
Rru_A1310: Rru_A1310 - Acetyl-CoA C-acyltransferase (NCBI), at 1,546,389 to 1,547,531
_A1310
Rru_A1311: Rru_A1311 - Transcriptional Regulator, MerR family (NCBI), at 1,547,584 to 1,547,982
_A1311
Rru_A1312: Rru_A1312 - AMP-dependent synthetase and ligase (NCBI), at 1,548,113 to 1,549,807
_A1312
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1545.501 kb on + strand, within Rru_A1309 at 1545.501 kb on + strand, within Rru_A1309 at 1545.502 kb on - strand, within Rru_A1309 at 1545.502 kb on - strand, within Rru_A1309 at 1545.550 kb on + strand, within Rru_A1309 at 1545.576 kb on + strand, within Rru_A1309 at 1545.576 kb on + strand, within Rru_A1309 at 1545.577 kb on - strand, within Rru_A1309 at 1545.577 kb on - strand, within Rru_A1309 at 1545.737 kb on - strand, within Rru_A1309 at 1545.737 kb on - strand, within Rru_A1309 at 1545.737 kb on - strand, within Rru_A1309 at 1545.814 kb on + strand, within Rru_A1309 at 1545.815 kb on - strand, within Rru_A1309 at 1545.922 kb on - strand, within Rru_A1309 at 1546.066 kb on - strand, within Rru_A1309 at 1546.132 kb on + strand, within Rru_A1309 at 1546.133 kb on - strand, within Rru_A1309 at 1546.133 kb on - strand, within Rru_A1309 at 1546.554 kb on - strand, within Rru_A1310 at 1546.563 kb on - strand, within Rru_A1310 at 1546.563 kb on - strand, within Rru_A1310 at 1546.563 kb on - strand, within Rru_A1310 at 1547.100 kb on - strand, within Rru_A1310 at 1547.101 kb on + strand, within Rru_A1310 at 1547.101 kb on + strand, within Rru_A1310 at 1547.101 kb on + strand, within Rru_A1310 at 1547.102 kb on - strand, within Rru_A1310 at 1547.458 kb on - strand at 1547.518 kb on - strand at 1547.518 kb on - strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.564 kb on + strand at 1547.565 kb on - strand at 1547.565 kb on - strand at 1547.769 kb on - strand, within Rru_A1311 at 1547.861 kb on + strand, within Rru_A1311 at 1547.989 kb on - strand at 1548.017 kb on + strand at 1548.265 kb on - strand at 1548.391 kb on - strand, within Rru_A1312 at 1548.426 kb on + strand, within Rru_A1312 at 1548.426 kb on + strand, within Rru_A1312 at 1548.474 kb on + strand, within Rru_A1312 at 1548.474 kb on + strand, within Rru_A1312 at 1548.474 kb on + strand, within Rru_A1312 at 1548.474 kb on + strand, within Rru_A1312 at 1548.474 kb on + strand, within Rru_A1312 at 1548.475 kb on - strand, within Rru_A1312 at 1548.475 kb on - strand, within Rru_A1312 at 1548.490 kb on + strand, within Rru_A1312 at 1548.492 kb on + strand, within Rru_A1312 at 1548.493 kb on - strand, within Rru_A1312
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 1,545,501 + Rru_A1309 0.62 -0.0 1,545,501 + Rru_A1309 0.62 -0.0 1,545,502 - Rru_A1309 0.62 -0.0 1,545,502 - Rru_A1309 0.62 -0.0 1,545,550 + Rru_A1309 0.64 -0.0 1,545,576 + Rru_A1309 0.66 -0.0 1,545,576 + Rru_A1309 0.66 -3.6 1,545,577 - Rru_A1309 0.66 -0.0 1,545,577 - Rru_A1309 0.66 -1.3 1,545,737 - Rru_A1309 0.73 -0.0 1,545,737 - Rru_A1309 0.73 -3.2 1,545,737 - Rru_A1309 0.73 -2.7 1,545,814 + Rru_A1309 0.76 -2.7 1,545,815 - Rru_A1309 0.76 -0.0 1,545,922 - Rru_A1309 0.80 -1.9 1,546,066 - Rru_A1309 0.87 -0.0 1,546,132 + Rru_A1309 0.90 -2.9 1,546,133 - Rru_A1309 0.90 -0.0 1,546,133 - Rru_A1309 0.90 -0.0 1,546,554 - Rru_A1310 0.14 -2.1 1,546,563 - Rru_A1310 0.15 -0.0 1,546,563 - Rru_A1310 0.15 -0.0 1,546,563 - Rru_A1310 0.15 -0.0 1,547,100 - Rru_A1310 0.62 -0.0 1,547,101 + Rru_A1310 0.62 -2.5 1,547,101 + Rru_A1310 0.62 -2.7 1,547,101 + Rru_A1310 0.62 -0.0 1,547,102 - Rru_A1310 0.62 -2.6 1,547,458 - -0.0 1,547,518 - -0.0 1,547,518 - -3.4 1,547,564 + -2.6 1,547,564 + -2.2 1,547,564 + -0.0 1,547,564 + -0.0 1,547,564 + -2.3 1,547,564 + -0.0 1,547,564 + -0.0 1,547,565 - -0.0 1,547,565 - -0.0 1,547,769 - Rru_A1311 0.46 -1.0 1,547,861 + Rru_A1311 0.69 -0.0 1,547,989 - -0.0 1,548,017 + -0.0 1,548,265 - -1.5 1,548,391 - Rru_A1312 0.16 -3.0 1,548,426 + Rru_A1312 0.18 -1.6 1,548,426 + Rru_A1312 0.18 -0.0 1,548,474 + Rru_A1312 0.21 -2.0 1,548,474 + Rru_A1312 0.21 -2.1 1,548,474 + Rru_A1312 0.21 -0.0 1,548,474 + Rru_A1312 0.21 -1.8 1,548,474 + Rru_A1312 0.21 -1.0 1,548,475 - Rru_A1312 0.21 -1.3 1,548,475 - Rru_A1312 0.21 -0.0 1,548,490 + Rru_A1312 0.22 -1.3 1,548,492 + Rru_A1312 0.22 -0.0 1,548,493 - Rru_A1312 0.22 -0.0
Or see this region's nucleotide sequence