Experiment: PYE with Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01447 and CCNA_01448 are separated by 74 nucleotides CCNA_01448 and CCNA_01449 are separated by 60 nucleotides
CCNA_01447: CCNA_01447 - homoserine dehydrogenase, at 1,559,503 to 1,560,792
_01447
CCNA_01448: CCNA_01448 - fructose-1,6-bisphosphatase, class II, at 1,560,867 to 1,561,820
_01448
CCNA_01449: CCNA_01449 - fructose-1,6-bisphosphatase, FIG superfamily, at 1,561,881 to 1,562,858
_01449
Position (kb)
1560
1561
1562 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8
9 at 1559.884 kb on - strand, within CCNA_01447 at 1559.886 kb on + strand, within CCNA_01447 at 1560.052 kb on + strand, within CCNA_01447 at 1560.052 kb on + strand, within CCNA_01447 at 1560.058 kb on + strand, within CCNA_01447 at 1560.058 kb on + strand, within CCNA_01447 at 1560.058 kb on + strand, within CCNA_01447 at 1560.058 kb on + strand, within CCNA_01447 at 1560.060 kb on - strand, within CCNA_01447 at 1560.060 kb on - strand, within CCNA_01447 at 1560.060 kb on - strand, within CCNA_01447 at 1560.063 kb on + strand, within CCNA_01447 at 1560.066 kb on - strand, within CCNA_01447 at 1560.066 kb on - strand, within CCNA_01447 at 1560.067 kb on - strand, within CCNA_01447 at 1560.194 kb on + strand, within CCNA_01447 at 1560.209 kb on + strand, within CCNA_01447 at 1560.219 kb on + strand, within CCNA_01447 at 1560.266 kb on + strand, within CCNA_01447 at 1560.267 kb on + strand, within CCNA_01447 at 1560.406 kb on - strand, within CCNA_01447 at 1560.415 kb on + strand, within CCNA_01447 at 1560.459 kb on + strand, within CCNA_01447 at 1560.459 kb on + strand, within CCNA_01447 at 1560.459 kb on + strand, within CCNA_01447 at 1560.459 kb on + strand, within CCNA_01447 at 1560.510 kb on - strand, within CCNA_01447 at 1560.536 kb on - strand, within CCNA_01447 at 1560.536 kb on - strand, within CCNA_01447 at 1560.548 kb on - strand, within CCNA_01447 at 1560.565 kb on + strand, within CCNA_01447 at 1560.605 kb on + strand, within CCNA_01447 at 1560.660 kb on + strand, within CCNA_01447 at 1560.828 kb on + strand at 1560.838 kb on + strand at 1560.956 kb on - strand at 1561.087 kb on - strand, within CCNA_01448 at 1561.180 kb on - strand, within CCNA_01448 at 1561.202 kb on + strand, within CCNA_01448 at 1561.215 kb on + strand, within CCNA_01448 at 1561.242 kb on - strand, within CCNA_01448 at 1561.328 kb on + strand, within CCNA_01448 at 1561.336 kb on - strand, within CCNA_01448 at 1561.336 kb on - strand, within CCNA_01448 at 1561.353 kb on + strand, within CCNA_01448 at 1561.413 kb on + strand, within CCNA_01448 at 1561.468 kb on - strand, within CCNA_01448 at 1561.481 kb on - strand, within CCNA_01448 at 1561.486 kb on + strand, within CCNA_01448 at 1561.493 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.535 kb on - strand, within CCNA_01448 at 1561.540 kb on - strand, within CCNA_01448 at 1561.663 kb on - strand, within CCNA_01448 at 1561.751 kb on + strand at 1561.753 kb on + strand at 1561.761 kb on - strand at 1561.761 kb on - strand at 1561.788 kb on + strand at 1561.796 kb on + strand at 1561.823 kb on - strand at 1561.823 kb on - strand at 1561.849 kb on - strand at 1561.949 kb on + strand at 1561.950 kb on + strand at 1561.950 kb on + strand at 1561.950 kb on + strand at 1561.994 kb on + strand, within CCNA_01449 at 1561.999 kb on + strand, within CCNA_01449 at 1562.030 kb on + strand, within CCNA_01449 at 1562.101 kb on + strand, within CCNA_01449 at 1562.153 kb on + strand, within CCNA_01449 at 1562.153 kb on + strand, within CCNA_01449 at 1562.330 kb on + strand, within CCNA_01449 at 1562.439 kb on - strand, within CCNA_01449 at 1562.693 kb on + strand, within CCNA_01449 at 1562.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Phosphomycin 0.005 mg/ml remove 1,559,884 - CCNA_01447 0.30 -1.1 1,559,886 + CCNA_01447 0.30 +2.9 1,560,052 + CCNA_01447 0.43 -0.6 1,560,052 + CCNA_01447 0.43 +2.3 1,560,058 + CCNA_01447 0.43 +0.8 1,560,058 + CCNA_01447 0.43 -0.6 1,560,058 + CCNA_01447 0.43 +1.8 1,560,058 + CCNA_01447 0.43 +1.3 1,560,060 - CCNA_01447 0.43 -0.2 1,560,060 - CCNA_01447 0.43 +0.5 1,560,060 - CCNA_01447 0.43 +0.5 1,560,063 + CCNA_01447 0.43 +0.8 1,560,066 - CCNA_01447 0.44 +1.3 1,560,066 - CCNA_01447 0.44 +2.8 1,560,067 - CCNA_01447 0.44 +0.3 1,560,194 + CCNA_01447 0.54 +0.1 1,560,209 + CCNA_01447 0.55 +1.1 1,560,219 + CCNA_01447 0.56 -0.9 1,560,266 + CCNA_01447 0.59 -0.1 1,560,267 + CCNA_01447 0.59 +0.5 1,560,406 - CCNA_01447 0.70 +0.0 1,560,415 + CCNA_01447 0.71 +2.2 1,560,459 + CCNA_01447 0.74 +1.9 1,560,459 + CCNA_01447 0.74 +0.3 1,560,459 + CCNA_01447 0.74 +0.3 1,560,459 + CCNA_01447 0.74 +1.0 1,560,510 - CCNA_01447 0.78 +0.5 1,560,536 - CCNA_01447 0.80 +0.5 1,560,536 - CCNA_01447 0.80 +3.0 1,560,548 - CCNA_01447 0.81 +4.8 1,560,565 + CCNA_01447 0.82 +1.8 1,560,605 + CCNA_01447 0.85 +2.7 1,560,660 + CCNA_01447 0.90 +0.5 1,560,828 + -0.8 1,560,838 + -0.7 1,560,956 - +0.0 1,561,087 - CCNA_01448 0.23 +0.5 1,561,180 - CCNA_01448 0.33 +0.8 1,561,202 + CCNA_01448 0.35 +0.8 1,561,215 + CCNA_01448 0.36 +0.1 1,561,242 - CCNA_01448 0.39 +1.0 1,561,328 + CCNA_01448 0.48 -0.7 1,561,336 - CCNA_01448 0.49 -0.2 1,561,336 - CCNA_01448 0.49 +1.3 1,561,353 + CCNA_01448 0.51 +6.3 1,561,413 + CCNA_01448 0.57 +3.2 1,561,468 - CCNA_01448 0.63 +1.8 1,561,481 - CCNA_01448 0.64 +2.8 1,561,486 + CCNA_01448 0.65 +0.7 1,561,493 + CCNA_01448 0.66 -0.2 1,561,527 + CCNA_01448 0.69 +1.1 1,561,527 + CCNA_01448 0.69 -0.2 1,561,527 + CCNA_01448 0.69 +1.7 1,561,535 - CCNA_01448 0.70 +1.8 1,561,540 - CCNA_01448 0.71 -0.2 1,561,663 - CCNA_01448 0.83 +0.3 1,561,751 + -1.5 1,561,753 + +0.0 1,561,761 - +2.8 1,561,761 - +1.8 1,561,788 + -1.6 1,561,796 + +2.4 1,561,823 - -0.6 1,561,823 - +2.8 1,561,849 - +2.8 1,561,949 + +7.8 1,561,950 + +5.7 1,561,950 + +8.6 1,561,950 + +7.9 1,561,994 + CCNA_01449 0.12 +5.7 1,561,999 + CCNA_01449 0.12 +6.0 1,562,030 + CCNA_01449 0.15 +8.3 1,562,101 + CCNA_01449 0.22 +8.2 1,562,153 + CCNA_01449 0.28 +6.1 1,562,153 + CCNA_01449 0.28 +7.6 1,562,330 + CCNA_01449 0.46 +6.9 1,562,439 - CCNA_01449 0.57 +6.2 1,562,693 + CCNA_01449 0.83 +9.0 1,562,779 + +7.3
Or see this region's nucleotide sequence