Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00157

Experiment: PYE with Phosphomycin 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00155 and CCNA_00156 are separated by 132 nucleotidesCCNA_00156 and CCNA_00157 are separated by 70 nucleotidesCCNA_00157 and CCNA_00158 are separated by 227 nucleotides CCNA_00155: CCNA_00155 - DNA polymerase III, beta chain, at 163,413 to 164,531 _00155 CCNA_00156: CCNA_00156 - ArsR-family transcriptional regulator, at 164,664 to 164,951 _00156 CCNA_00157: CCNA_00157 - glyoxalase family protein, at 165,022 to 165,393 _00157 CCNA_00158: CCNA_00158 - DNA replication and repair protein recF, at 165,621 to 166,784 _00158 Position (kb) 165 166Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 164.525 kb on + strandat 164.525 kb on + strandat 164.609 kb on + strandat 164.609 kb on + strandat 164.612 kb on + strandat 164.612 kb on - strandat 164.618 kb on + strandat 164.627 kb on - strandat 164.627 kb on - strandat 164.637 kb on + strandat 164.645 kb on - strandat 164.650 kb on - strandat 164.694 kb on + strand, within CCNA_00156at 164.696 kb on + strand, within CCNA_00156at 164.696 kb on + strand, within CCNA_00156at 164.696 kb on + strand, within CCNA_00156at 164.703 kb on + strand, within CCNA_00156at 164.704 kb on - strand, within CCNA_00156at 164.704 kb on - strand, within CCNA_00156at 164.711 kb on - strand, within CCNA_00156at 164.712 kb on + strand, within CCNA_00156at 164.738 kb on - strand, within CCNA_00156at 164.745 kb on + strand, within CCNA_00156at 164.745 kb on + strand, within CCNA_00156at 164.750 kb on + strand, within CCNA_00156at 164.753 kb on - strand, within CCNA_00156at 164.781 kb on + strand, within CCNA_00156at 164.793 kb on + strand, within CCNA_00156at 164.798 kb on + strand, within CCNA_00156at 164.803 kb on + strand, within CCNA_00156at 164.823 kb on + strand, within CCNA_00156at 164.868 kb on + strand, within CCNA_00156at 164.868 kb on + strand, within CCNA_00156at 164.868 kb on + strand, within CCNA_00156at 164.876 kb on - strand, within CCNA_00156at 164.876 kb on - strand, within CCNA_00156at 164.876 kb on - strand, within CCNA_00156at 164.911 kb on + strand, within CCNA_00156at 164.913 kb on + strand, within CCNA_00156at 164.919 kb on - strand, within CCNA_00156at 164.943 kb on - strandat 164.943 kb on - strandat 164.944 kb on - strandat 164.946 kb on + strandat 165.018 kb on + strandat 165.018 kb on + strandat 165.018 kb on + strandat 165.026 kb on - strandat 165.026 kb on - strandat 165.026 kb on - strandat 165.033 kb on + strandat 165.071 kb on - strand, within CCNA_00157at 165.077 kb on - strand, within CCNA_00157at 165.077 kb on - strand, within CCNA_00157at 165.162 kb on - strand, within CCNA_00157at 165.253 kb on - strand, within CCNA_00157at 165.263 kb on - strand, within CCNA_00157at 165.278 kb on + strand, within CCNA_00157at 165.278 kb on + strand, within CCNA_00157at 165.286 kb on - strand, within CCNA_00157at 165.286 kb on - strand, within CCNA_00157at 165.295 kb on + strand, within CCNA_00157at 165.297 kb on + strand, within CCNA_00157at 165.305 kb on - strand, within CCNA_00157at 165.307 kb on - strand, within CCNA_00157at 165.325 kb on + strand, within CCNA_00157at 165.330 kb on + strand, within CCNA_00157at 165.338 kb on - strand, within CCNA_00157at 165.377 kb on + strandat 165.377 kb on - strandat 165.385 kb on - strandat 165.421 kb on - strandat 165.428 kb on + strandat 165.447 kb on + strandat 165.466 kb on - strandat 165.506 kb on - strandat 165.522 kb on - strandat 165.522 kb on - strandat 165.522 kb on - strandat 165.536 kb on + strandat 165.554 kb on - strandat 165.564 kb on - strandat 165.582 kb on + strandat 165.582 kb on + strandat 165.582 kb on + strandat 165.595 kb on + strandat 165.614 kb on + strandat 165.681 kb on + strandat 165.683 kb on + strandat 165.711 kb on - strandat 165.711 kb on - strandat 165.711 kb on - strandat 165.711 kb on - strandat 165.727 kb on + strandat 165.744 kb on - strand, within CCNA_00158at 165.764 kb on + strand, within CCNA_00158at 165.764 kb on + strand, within CCNA_00158at 165.764 kb on + strand, within CCNA_00158at 165.767 kb on - strand, within CCNA_00158at 165.772 kb on - strand, within CCNA_00158at 165.772 kb on - strand, within CCNA_00158at 165.772 kb on - strand, within CCNA_00158at 165.777 kb on - strand, within CCNA_00158at 165.780 kb on + strand, within CCNA_00158at 165.780 kb on + strand, within CCNA_00158at 165.787 kb on - strand, within CCNA_00158at 165.790 kb on + strand, within CCNA_00158at 165.791 kb on + strand, within CCNA_00158at 165.796 kb on + strand, within CCNA_00158at 165.824 kb on + strand, within CCNA_00158at 165.842 kb on + strand, within CCNA_00158at 165.842 kb on + strand, within CCNA_00158at 165.842 kb on + strand, within CCNA_00158at 165.842 kb on + strand, within CCNA_00158at 165.845 kb on - strand, within CCNA_00158at 165.847 kb on + strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.850 kb on - strand, within CCNA_00158at 165.857 kb on - strand, within CCNA_00158at 165.864 kb on + strand, within CCNA_00158at 165.881 kb on - strand, within CCNA_00158at 165.900 kb on - strand, within CCNA_00158at 165.903 kb on + strand, within CCNA_00158at 165.911 kb on - strand, within CCNA_00158at 165.912 kb on - strand, within CCNA_00158at 165.917 kb on - strand, within CCNA_00158at 165.917 kb on - strand, within CCNA_00158at 165.917 kb on - strand, within CCNA_00158at 165.917 kb on - strand, within CCNA_00158at 165.947 kb on + strand, within CCNA_00158at 165.978 kb on + strand, within CCNA_00158at 166.005 kb on - strand, within CCNA_00158at 166.036 kb on + strand, within CCNA_00158at 166.085 kb on + strand, within CCNA_00158at 166.107 kb on + strand, within CCNA_00158at 166.115 kb on - strand, within CCNA_00158at 166.115 kb on - strand, within CCNA_00158at 166.179 kb on - strand, within CCNA_00158at 166.195 kb on + strand, within CCNA_00158at 166.203 kb on - strand, within CCNA_00158at 166.227 kb on + strand, within CCNA_00158at 166.227 kb on + strand, within CCNA_00158at 166.227 kb on + strand, within CCNA_00158at 166.235 kb on - strand, within CCNA_00158at 166.235 kb on - strand, within CCNA_00158at 166.235 kb on - strand, within CCNA_00158at 166.268 kb on + strand, within CCNA_00158at 166.312 kb on + strand, within CCNA_00158at 166.312 kb on + strand, within CCNA_00158at 166.312 kb on + strand, within CCNA_00158at 166.337 kb on - strand, within CCNA_00158at 166.339 kb on - strand, within CCNA_00158at 166.344 kb on + strand, within CCNA_00158at 166.344 kb on + strand, within CCNA_00158at 166.344 kb on + strand, within CCNA_00158at 166.352 kb on - strand, within CCNA_00158at 166.352 kb on - strand, within CCNA_00158at 166.391 kb on + strand, within CCNA_00158

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Phosphomycin 0.005 mg/ml
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164,525 + -0.0
164,525 + -0.1
164,609 + -0.6
164,609 + -0.8
164,612 + -0.3
164,612 - -1.7
164,618 + -0.6
164,627 - -1.0
164,627 - -1.6
164,637 + -1.6
164,645 - +2.4
164,650 - -0.7
164,694 + CCNA_00156 0.10 -0.4
164,696 + CCNA_00156 0.11 +0.3
164,696 + CCNA_00156 0.11 -2.0
164,696 + CCNA_00156 0.11 -0.2
164,703 + CCNA_00156 0.14 -1.3
164,704 - CCNA_00156 0.14 -2.7
164,704 - CCNA_00156 0.14 +1.0
164,711 - CCNA_00156 0.16 -2.0
164,712 + CCNA_00156 0.17 +0.2
164,738 - CCNA_00156 0.26 -2.1
164,745 + CCNA_00156 0.28 +1.4
164,745 + CCNA_00156 0.28 -1.8
164,750 + CCNA_00156 0.30 +2.0
164,753 - CCNA_00156 0.31 -2.0
164,781 + CCNA_00156 0.41 -0.0
164,793 + CCNA_00156 0.45 -2.5
164,798 + CCNA_00156 0.47 -1.7
164,803 + CCNA_00156 0.48 -0.8
164,823 + CCNA_00156 0.55 +2.2
164,868 + CCNA_00156 0.71 -0.9
164,868 + CCNA_00156 0.71 -1.2
164,868 + CCNA_00156 0.71 +2.4
164,876 - CCNA_00156 0.74 -0.4
164,876 - CCNA_00156 0.74 +0.3
164,876 - CCNA_00156 0.74 +1.6
164,911 + CCNA_00156 0.86 -1.6
164,913 + CCNA_00156 0.86 -1.3
164,919 - CCNA_00156 0.89 +0.2
164,943 - +0.3
164,943 - -0.6
164,944 - -1.6
164,946 + +0.4
165,018 + -2.6
165,018 + -1.1
165,018 + +0.8
165,026 - -0.4
165,026 - -0.2
165,026 - +0.3
165,033 + -0.6
165,071 - CCNA_00157 0.13 +0.6
165,077 - CCNA_00157 0.15 +1.6
165,077 - CCNA_00157 0.15 +1.6
165,162 - CCNA_00157 0.38 -0.2
165,253 - CCNA_00157 0.62 +1.0
165,263 - CCNA_00157 0.65 +1.0
165,278 + CCNA_00157 0.69 -1.7
165,278 + CCNA_00157 0.69 -1.1
165,286 - CCNA_00157 0.71 +0.6
165,286 - CCNA_00157 0.71 +2.6
165,295 + CCNA_00157 0.73 -0.3
165,297 + CCNA_00157 0.74 -1.0
165,305 - CCNA_00157 0.76 -1.2
165,307 - CCNA_00157 0.77 -1.5
165,325 + CCNA_00157 0.81 +0.0
165,330 + CCNA_00157 0.83 -1.4
165,338 - CCNA_00157 0.85 -0.8
165,377 + -0.8
165,377 - -0.3
165,385 - -0.2
165,421 - +2.6
165,428 + -0.5
165,447 + +0.0
165,466 - -0.2
165,506 - -1.0
165,522 - -2.7
165,522 - -0.7
165,522 - -1.3
165,536 + -1.4
165,554 - -0.8
165,564 - +2.3
165,582 + -1.0
165,582 + +0.9
165,582 + -0.8
165,595 + +0.3
165,614 + +1.3
165,681 + -1.9
165,683 + +2.2
165,711 - +0.3
165,711 - -1.0
165,711 - +2.6
165,711 - +0.6
165,727 + -0.0
165,744 - CCNA_00158 0.11 -1.6
165,764 + CCNA_00158 0.12 -0.2
165,764 + CCNA_00158 0.12 -0.4
165,764 + CCNA_00158 0.12 +0.1
165,767 - CCNA_00158 0.13 -0.4
165,772 - CCNA_00158 0.13 +0.5
165,772 - CCNA_00158 0.13 +0.7
165,772 - CCNA_00158 0.13 +0.8
165,777 - CCNA_00158 0.13 -0.2
165,780 + CCNA_00158 0.14 +0.8
165,780 + CCNA_00158 0.14 +1.0
165,787 - CCNA_00158 0.14 -0.4
165,790 + CCNA_00158 0.15 +1.2
165,791 + CCNA_00158 0.15 -1.6
165,796 + CCNA_00158 0.15 -1.3
165,824 + CCNA_00158 0.17 +0.9
165,842 + CCNA_00158 0.19 +0.1
165,842 + CCNA_00158 0.19 -1.7
165,842 + CCNA_00158 0.19 +0.1
165,842 + CCNA_00158 0.19 -0.1
165,845 - CCNA_00158 0.19 -1.5
165,847 + CCNA_00158 0.19 -1.6
165,850 - CCNA_00158 0.20 +0.0
165,850 - CCNA_00158 0.20 +0.3
165,850 - CCNA_00158 0.20 -1.2
165,850 - CCNA_00158 0.20 -2.3
165,850 - CCNA_00158 0.20 +1.0
165,850 - CCNA_00158 0.20 -0.8
165,850 - CCNA_00158 0.20 +0.6
165,850 - CCNA_00158 0.20 +0.6
165,857 - CCNA_00158 0.20 +1.0
165,864 + CCNA_00158 0.21 -0.6
165,881 - CCNA_00158 0.22 -1.1
165,900 - CCNA_00158 0.24 -0.8
165,903 + CCNA_00158 0.24 +0.4
165,911 - CCNA_00158 0.25 +0.0
165,912 - CCNA_00158 0.25 +1.7
165,917 - CCNA_00158 0.25 +0.6
165,917 - CCNA_00158 0.25 +1.3
165,917 - CCNA_00158 0.25 +0.3
165,917 - CCNA_00158 0.25 -0.3
165,947 + CCNA_00158 0.28 -1.2
165,978 + CCNA_00158 0.31 +2.3
166,005 - CCNA_00158 0.33 -2.0
166,036 + CCNA_00158 0.36 +0.2
166,085 + CCNA_00158 0.40 +1.2
166,107 + CCNA_00158 0.42 -0.8
166,115 - CCNA_00158 0.42 +3.7
166,115 - CCNA_00158 0.42 -0.2
166,179 - CCNA_00158 0.48 +1.3
166,195 + CCNA_00158 0.49 -1.7
166,203 - CCNA_00158 0.50 -0.6
166,227 + CCNA_00158 0.52 +0.0
166,227 + CCNA_00158 0.52 +0.7
166,227 + CCNA_00158 0.52 +2.5
166,235 - CCNA_00158 0.53 +0.3
166,235 - CCNA_00158 0.53 -0.2
166,235 - CCNA_00158 0.53 +2.6
166,268 + CCNA_00158 0.56 +1.3
166,312 + CCNA_00158 0.59 -0.0
166,312 + CCNA_00158 0.59 -0.1
166,312 + CCNA_00158 0.59 -1.5
166,337 - CCNA_00158 0.62 -2.8
166,339 - CCNA_00158 0.62 +1.2
166,344 + CCNA_00158 0.62 +1.0
166,344 + CCNA_00158 0.62 -1.9
166,344 + CCNA_00158 0.62 -1.8
166,352 - CCNA_00158 0.63 -1.2
166,352 - CCNA_00158 0.63 +0.3
166,391 + CCNA_00158 0.66 +0.6

Or see this region's nucleotide sequence