Experiment: PYE with Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00145 and CCNA_00146 are separated by 24 nucleotides CCNA_00146 and CCNA_00147 are separated by 167 nucleotides CCNA_00147 and CCNA_00148 are separated by 75 nucleotides CCNA_00148 and CCNA_00149 are separated by 16 nucleotides CCNA_00149 and CCNA_00150 overlap by 4 nucleotides
CCNA_00145: CCNA_00145 - xanthosine triphosphate pyrophosphatase, at 156,966 to 157,568
_00145
CCNA_00146: CCNA_00146 - glutathione S-transferase, at 157,593 to 158,186
_00146
CCNA_00147: CCNA_00147 - hypothetical protein, at 158,354 to 158,470
_00147
CCNA_00148: CCNA_00148 - hypothetical protein, at 158,546 to 159,079
_00148
CCNA_00149: CCNA_00149 - Cro/CI-family transcriptional regulator, at 159,096 to 159,323
_00149
CCNA_00150: CCNA_00150 - PDZ domain family protein, at 159,320 to 160,297
_00150
Position (kb) 158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 157.364 kb on + strand, within CCNA_00145 at 157.364 kb on + strand, within CCNA_00145 at 157.373 kb on + strand, within CCNA_00145 at 157.374 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.390 kb on + strand, within CCNA_00145 at 157.390 kb on + strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.434 kb on - strand, within CCNA_00145 at 157.445 kb on + strand, within CCNA_00145 at 157.500 kb on + strand, within CCNA_00145 at 157.516 kb on + strand at 157.517 kb on + strand at 157.538 kb on + strand at 157.550 kb on + strand at 157.550 kb on + strand at 157.681 kb on - strand, within CCNA_00146 at 157.695 kb on - strand, within CCNA_00146 at 157.705 kb on - strand, within CCNA_00146 at 157.741 kb on - strand, within CCNA_00146 at 157.748 kb on - strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.775 kb on + strand, within CCNA_00146 at 157.781 kb on - strand, within CCNA_00146 at 157.814 kb on + strand, within CCNA_00146 at 157.814 kb on + strand, within CCNA_00146 at 157.860 kb on + strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.887 kb on + strand, within CCNA_00146 at 157.902 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.956 kb on - strand, within CCNA_00146 at 157.956 kb on - strand, within CCNA_00146 at 157.965 kb on + strand, within CCNA_00146 at 157.978 kb on - strand, within CCNA_00146 at 158.016 kb on - strand, within CCNA_00146 at 158.071 kb on + strand, within CCNA_00146 at 158.079 kb on - strand, within CCNA_00146 at 158.124 kb on + strand, within CCNA_00146 at 158.128 kb on + strand at 158.139 kb on + strand at 158.147 kb on - strand at 158.149 kb on - strand at 158.172 kb on - strand at 158.174 kb on + strand at 158.181 kb on + strand at 158.181 kb on + strand at 158.189 kb on - strand at 158.194 kb on - strand at 158.291 kb on + strand at 158.294 kb on - strand at 158.299 kb on - strand at 158.299 kb on - strand at 158.309 kb on - strand at 158.334 kb on + strand at 158.366 kb on + strand, within CCNA_00147 at 158.434 kb on + strand, within CCNA_00147 at 158.463 kb on + strand at 158.463 kb on + strand at 158.464 kb on - strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.471 kb on - strand at 158.471 kb on - strand at 158.492 kb on - strand at 158.492 kb on - strand at 158.517 kb on + strand at 158.532 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand at 158.602 kb on + strand, within CCNA_00148 at 158.620 kb on - strand, within CCNA_00148 at 158.652 kb on - strand, within CCNA_00148 at 158.668 kb on - strand, within CCNA_00148 at 158.670 kb on + strand, within CCNA_00148 at 158.670 kb on - strand, within CCNA_00148 at 158.673 kb on - strand, within CCNA_00148 at 158.707 kb on + strand, within CCNA_00148 at 158.738 kb on + strand, within CCNA_00148 at 158.764 kb on + strand, within CCNA_00148 at 158.772 kb on - strand, within CCNA_00148 at 158.774 kb on + strand, within CCNA_00148 at 158.813 kb on + strand, within CCNA_00148 at 158.819 kb on + strand, within CCNA_00148 at 158.889 kb on - strand, within CCNA_00148 at 158.901 kb on + strand, within CCNA_00148 at 158.956 kb on + strand, within CCNA_00148 at 158.957 kb on + strand, within CCNA_00148 at 158.964 kb on - strand, within CCNA_00148 at 158.982 kb on - strand, within CCNA_00148 at 159.076 kb on - strand at 159.078 kb on + strand at 159.078 kb on + strand at 159.111 kb on + strand at 159.119 kb on - strand, within CCNA_00149 at 159.124 kb on + strand, within CCNA_00149 at 159.132 kb on - strand, within CCNA_00149 at 159.166 kb on - strand, within CCNA_00149 at 159.188 kb on + strand, within CCNA_00149 at 159.196 kb on - strand, within CCNA_00149 at 159.203 kb on - strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.230 kb on - strand, within CCNA_00149 at 159.266 kb on - strand, within CCNA_00149 at 159.279 kb on + strand, within CCNA_00149 at 159.345 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.354 kb on - strand at 159.354 kb on - strand at 159.370 kb on + strand at 159.391 kb on - strand at 159.402 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Phosphomycin 0.005 mg/ml remove 157,364 + CCNA_00145 0.66 +0.3 157,364 + CCNA_00145 0.66 -2.6 157,373 + CCNA_00145 0.67 +1.8 157,374 - CCNA_00145 0.68 +0.0 157,381 - CCNA_00145 0.69 +0.3 157,381 - CCNA_00145 0.69 +0.0 157,381 - CCNA_00145 0.69 +0.1 157,390 + CCNA_00145 0.70 -1.9 157,390 + CCNA_00145 0.70 +0.6 157,398 - CCNA_00145 0.72 +0.0 157,398 - CCNA_00145 0.72 +0.1 157,398 - CCNA_00145 0.72 -0.3 157,398 - CCNA_00145 0.72 +0.6 157,398 - CCNA_00145 0.72 +0.3 157,398 - CCNA_00145 0.72 +0.6 157,434 - CCNA_00145 0.78 +0.8 157,445 + CCNA_00145 0.79 +2.6 157,500 + CCNA_00145 0.89 +0.6 157,516 + -0.8 157,517 + -0.9 157,538 + +2.4 157,550 + -1.1 157,550 + +0.3 157,681 - CCNA_00146 0.15 +0.0 157,695 - CCNA_00146 0.17 -1.1 157,705 - CCNA_00146 0.19 -1.7 157,741 - CCNA_00146 0.25 +1.0 157,748 - CCNA_00146 0.26 -1.6 157,773 + CCNA_00146 0.30 -1.1 157,773 + CCNA_00146 0.30 -0.7 157,773 + CCNA_00146 0.30 -2.2 157,775 + CCNA_00146 0.31 -1.1 157,781 - CCNA_00146 0.32 -1.7 157,814 + CCNA_00146 0.37 +0.6 157,814 + CCNA_00146 0.37 +1.1 157,860 + CCNA_00146 0.45 -2.4 157,868 - CCNA_00146 0.46 +0.0 157,868 - CCNA_00146 0.46 +0.8 157,868 - CCNA_00146 0.46 +2.6 157,868 - CCNA_00146 0.46 -0.9 157,868 - CCNA_00146 0.46 -0.5 157,887 + CCNA_00146 0.49 -1.5 157,902 + CCNA_00146 0.52 +0.2 157,948 + CCNA_00146 0.60 +0.0 157,948 + CCNA_00146 0.60 -0.4 157,948 + CCNA_00146 0.60 -1.4 157,956 - CCNA_00146 0.61 -0.7 157,956 - CCNA_00146 0.61 -1.9 157,965 + CCNA_00146 0.63 +1.2 157,978 - CCNA_00146 0.65 -1.2 158,016 - CCNA_00146 0.71 -0.1 158,071 + CCNA_00146 0.80 +0.3 158,079 - CCNA_00146 0.82 +1.0 158,124 + CCNA_00146 0.89 -1.5 158,128 + -0.6 158,139 + -0.9 158,147 - +1.1 158,149 - -2.4 158,172 - -1.7 158,174 + -0.2 158,181 + +0.3 158,181 + +0.6 158,189 - +0.3 158,194 - +0.6 158,291 + +2.3 158,294 - +3.6 158,299 - +1.0 158,299 - +2.6 158,309 - -1.2 158,334 + -3.1 158,366 + CCNA_00147 0.10 +2.2 158,434 + CCNA_00147 0.68 +0.1 158,463 + -0.8 158,463 + +0.6 158,464 - -0.6 158,465 + +0.4 158,465 + +0.6 158,465 + -0.8 158,465 + -1.6 158,471 - -1.2 158,471 - +2.9 158,492 - -1.0 158,492 - +0.6 158,517 + -0.1 158,532 + -0.4 158,553 + +0.4 158,553 + -1.1 158,553 + +0.2 158,602 + CCNA_00148 0.10 -1.6 158,620 - CCNA_00148 0.14 -1.5 158,652 - CCNA_00148 0.20 -1.0 158,668 - CCNA_00148 0.23 -0.2 158,670 + CCNA_00148 0.23 -0.3 158,670 - CCNA_00148 0.23 -1.9 158,673 - CCNA_00148 0.24 +0.0 158,707 + CCNA_00148 0.30 -0.5 158,738 + CCNA_00148 0.36 -1.1 158,764 + CCNA_00148 0.41 -0.5 158,772 - CCNA_00148 0.42 +0.3 158,774 + CCNA_00148 0.43 -2.0 158,813 + CCNA_00148 0.50 -2.0 158,819 + CCNA_00148 0.51 -0.4 158,889 - CCNA_00148 0.64 -1.2 158,901 + CCNA_00148 0.66 -0.1 158,956 + CCNA_00148 0.77 -2.4 158,957 + CCNA_00148 0.77 -0.5 158,964 - CCNA_00148 0.78 -2.4 158,982 - CCNA_00148 0.82 -1.3 159,076 - -1.7 159,078 + -1.9 159,078 + -2.5 159,111 + +1.6 159,119 - CCNA_00149 0.10 +0.3 159,124 + CCNA_00149 0.12 -2.5 159,132 - CCNA_00149 0.16 -2.2 159,166 - CCNA_00149 0.31 -2.2 159,188 + CCNA_00149 0.40 -0.7 159,196 - CCNA_00149 0.44 +1.0 159,203 - CCNA_00149 0.47 +2.6 159,227 + CCNA_00149 0.57 -0.4 159,227 + CCNA_00149 0.57 -1.2 159,227 + CCNA_00149 0.57 -0.3 159,230 - CCNA_00149 0.59 -2.2 159,266 - CCNA_00149 0.75 -1.4 159,279 + CCNA_00149 0.80 -1.7 159,345 + -1.3 159,346 + +1.3 159,346 + +0.6 159,354 - +0.0 159,354 - -0.5 159,370 + -2.3 159,391 - -0.6 159,402 + +0.7
Or see this region's nucleotide sequence