Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00001

Experiment: PYE with Phosphomycin 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00001 and CCNA_00002 overlap by 4 nucleotidesCCNA_00002 and CCNA_00003 overlap by 4 nucleotides CCNA_00001: CCNA_00001 - ATP/GTP-binding protein, at 202 to 1,107 _00001 CCNA_00002: CCNA_00002 - septum formation protein Maf, at 1,104 to 1,703 _00002 CCNA_00003: CCNA_00003 - shikimate dehydrogenase (EC 1.1.1.25) (from data), at 1,700 to 2,557 _00003 Position (kb) 0 1 2Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 0.042 kb on + strandat 0.194 kb on - strandat 0.194 kb on - strandat 0.196 kb on + strandat 0.239 kb on + strandat 0.249 kb on + strandat 0.255 kb on + strandat 0.257 kb on - strandat 0.304 kb on + strand, within CCNA_00001at 0.387 kb on + strand, within CCNA_00001at 0.443 kb on + strand, within CCNA_00001at 0.455 kb on + strand, within CCNA_00001at 0.504 kb on - strand, within CCNA_00001at 0.588 kb on + strand, within CCNA_00001at 0.649 kb on + strand, within CCNA_00001at 0.649 kb on + strand, within CCNA_00001at 0.705 kb on + strand, within CCNA_00001at 0.705 kb on + strand, within CCNA_00001at 0.718 kb on - strand, within CCNA_00001at 0.738 kb on - strand, within CCNA_00001at 0.748 kb on - strand, within CCNA_00001at 0.829 kb on - strand, within CCNA_00001at 1.036 kb on + strandat 1.036 kb on + strandat 1.067 kb on - strandat 1.078 kb on + strandat 1.079 kb on - strandat 1.079 kb on - strandat 1.083 kb on + strandat 1.083 kb on + strandat 1.086 kb on - strandat 1.086 kb on - strandat 1.091 kb on - strandat 1.096 kb on + strandat 1.096 kb on - strandat 1.104 kb on - strandat 1.114 kb on + strandat 1.114 kb on + strandat 1.114 kb on + strandat 1.131 kb on + strandat 1.131 kb on + strandat 1.155 kb on + strandat 1.192 kb on + strand, within CCNA_00002at 1.220 kb on + strand, within CCNA_00002at 1.248 kb on + strand, within CCNA_00002at 1.261 kb on + strand, within CCNA_00002at 1.261 kb on + strand, within CCNA_00002at 1.292 kb on + strand, within CCNA_00002at 1.293 kb on + strand, within CCNA_00002at 1.293 kb on - strand, within CCNA_00002at 1.300 kb on - strand, within CCNA_00002at 1.300 kb on - strand, within CCNA_00002at 1.319 kb on + strand, within CCNA_00002at 1.324 kb on + strand, within CCNA_00002at 1.324 kb on + strand, within CCNA_00002at 1.327 kb on - strand, within CCNA_00002at 1.329 kb on + strand, within CCNA_00002at 1.329 kb on + strand, within CCNA_00002at 1.337 kb on - strand, within CCNA_00002at 1.337 kb on - strand, within CCNA_00002at 1.342 kb on + strand, within CCNA_00002at 1.350 kb on - strand, within CCNA_00002at 1.364 kb on - strand, within CCNA_00002at 1.374 kb on + strand, within CCNA_00002at 1.386 kb on + strand, within CCNA_00002at 1.399 kb on + strand, within CCNA_00002at 1.399 kb on + strand, within CCNA_00002at 1.399 kb on + strand, within CCNA_00002at 1.399 kb on + strand, within CCNA_00002at 1.399 kb on + strand, within CCNA_00002at 1.401 kb on + strand, within CCNA_00002at 1.407 kb on - strand, within CCNA_00002at 1.407 kb on - strand, within CCNA_00002at 1.407 kb on - strand, within CCNA_00002at 1.407 kb on - strand, within CCNA_00002at 1.407 kb on - strand, within CCNA_00002at 1.417 kb on - strand, within CCNA_00002at 1.428 kb on - strand, within CCNA_00002at 1.459 kb on + strand, within CCNA_00002at 1.467 kb on + strand, within CCNA_00002at 1.486 kb on - strand, within CCNA_00002at 1.531 kb on + strand, within CCNA_00002at 1.531 kb on + strand, within CCNA_00002at 1.531 kb on + strand, within CCNA_00002at 1.531 kb on + strand, within CCNA_00002at 1.531 kb on + strand, within CCNA_00002at 1.534 kb on + strand, within CCNA_00002at 1.534 kb on + strand, within CCNA_00002at 1.534 kb on + strand, within CCNA_00002at 1.534 kb on + strand, within CCNA_00002at 1.539 kb on - strand, within CCNA_00002at 1.539 kb on - strand, within CCNA_00002at 1.542 kb on - strand, within CCNA_00002at 1.542 kb on - strand, within CCNA_00002at 1.542 kb on - strand, within CCNA_00002at 1.542 kb on - strand, within CCNA_00002at 1.542 kb on - strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.554 kb on + strand, within CCNA_00002at 1.559 kb on + strand, within CCNA_00002at 1.559 kb on + strand, within CCNA_00002at 1.562 kb on - strand, within CCNA_00002at 1.562 kb on - strand, within CCNA_00002at 1.562 kb on - strand, within CCNA_00002at 1.562 kb on - strand, within CCNA_00002at 1.567 kb on - strand, within CCNA_00002at 1.567 kb on - strand, within CCNA_00002at 1.567 kb on - strand, within CCNA_00002at 1.576 kb on + strand, within CCNA_00002at 1.576 kb on + strand, within CCNA_00002at 1.576 kb on + strand, within CCNA_00002at 1.581 kb on + strand, within CCNA_00002at 1.581 kb on + strand, within CCNA_00002at 1.581 kb on - strand, within CCNA_00002at 1.589 kb on - strand, within CCNA_00002at 1.591 kb on - strand, within CCNA_00002at 1.605 kb on - strand, within CCNA_00002at 1.610 kb on + strand, within CCNA_00002at 1.639 kb on + strand, within CCNA_00002at 1.647 kb on - strandat 1.663 kb on - strandat 1.689 kb on + strandat 1.696 kb on + strandat 1.697 kb on - strandat 1.697 kb on - strandat 1.739 kb on + strandat 1.739 kb on + strandat 1.747 kb on - strandat 1.751 kb on - strandat 1.768 kb on - strandat 1.814 kb on - strand, within CCNA_00003at 1.817 kb on + strand, within CCNA_00003at 1.849 kb on + strand, within CCNA_00003at 1.849 kb on + strand, within CCNA_00003at 1.901 kb on - strand, within CCNA_00003at 1.905 kb on + strand, within CCNA_00003at 1.919 kb on + strand, within CCNA_00003at 1.980 kb on + strand, within CCNA_00003at 2.039 kb on - strand, within CCNA_00003at 2.044 kb on - strand, within CCNA_00003at 2.077 kb on - strand, within CCNA_00003

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Phosphomycin 0.005 mg/ml
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42 + +2.6
194 - -1.2
194 - -2.1
196 + -1.9
239 + +0.3
249 + -2.3
255 + +2.2
257 - -0.7
304 + CCNA_00001 0.11 +2.6
387 + CCNA_00001 0.20 +4.2
443 + CCNA_00001 0.27 +3.3
455 + CCNA_00001 0.28 +4.5
504 - CCNA_00001 0.33 +4.6
588 + CCNA_00001 0.43 +4.6
649 + CCNA_00001 0.49 +6.7
649 + CCNA_00001 0.49 +5.3
705 + CCNA_00001 0.56 +0.6
705 + CCNA_00001 0.56 +3.3
718 - CCNA_00001 0.57 +2.0
738 - CCNA_00001 0.59 +1.0
748 - CCNA_00001 0.60 +1.0
829 - CCNA_00001 0.69 +2.6
1,036 + +1.6
1,036 + +4.3
1,067 - +2.6
1,078 + +0.6
1,079 - +1.6
1,079 - +0.3
1,083 + -2.9
1,083 + -1.1
1,086 - +0.0
1,086 - -0.2
1,091 - -0.4
1,096 + +5.6
1,096 - -1.2
1,104 - +1.6
1,114 + -1.0
1,114 + -2.8
1,114 + -2.3
1,131 + +1.6
1,131 + +1.3
1,155 + -0.9
1,192 + CCNA_00002 0.15 -1.4
1,220 + CCNA_00002 0.19 +2.6
1,248 + CCNA_00002 0.24 -0.2
1,261 + CCNA_00002 0.26 +0.2
1,261 + CCNA_00002 0.26 -0.3
1,292 + CCNA_00002 0.31 +0.6
1,293 + CCNA_00002 0.32 -0.3
1,293 - CCNA_00002 0.32 -0.7
1,300 - CCNA_00002 0.33 +1.0
1,300 - CCNA_00002 0.33 -0.2
1,319 + CCNA_00002 0.36 +0.1
1,324 + CCNA_00002 0.37 +0.1
1,324 + CCNA_00002 0.37 +4.2
1,327 - CCNA_00002 0.37 -0.3
1,329 + CCNA_00002 0.38 +0.1
1,329 + CCNA_00002 0.38 -1.9
1,337 - CCNA_00002 0.39 +1.3
1,337 - CCNA_00002 0.39 +2.6
1,342 + CCNA_00002 0.40 -1.0
1,350 - CCNA_00002 0.41 +0.4
1,364 - CCNA_00002 0.43 +0.5
1,374 + CCNA_00002 0.45 +0.1
1,386 + CCNA_00002 0.47 -1.7
1,399 + CCNA_00002 0.49 -0.9
1,399 + CCNA_00002 0.49 +2.6
1,399 + CCNA_00002 0.49 +0.3
1,399 + CCNA_00002 0.49 -1.2
1,399 + CCNA_00002 0.49 -0.7
1,401 + CCNA_00002 0.49 -1.3
1,407 - CCNA_00002 0.51 -0.4
1,407 - CCNA_00002 0.51 +2.0
1,407 - CCNA_00002 0.51 -0.7
1,407 - CCNA_00002 0.51 +0.1
1,407 - CCNA_00002 0.51 -1.4
1,417 - CCNA_00002 0.52 -0.7
1,428 - CCNA_00002 0.54 +1.4
1,459 + CCNA_00002 0.59 -2.9
1,467 + CCNA_00002 0.60 -1.0
1,486 - CCNA_00002 0.64 -1.2
1,531 + CCNA_00002 0.71 -1.3
1,531 + CCNA_00002 0.71 +0.6
1,531 + CCNA_00002 0.71 -0.2
1,531 + CCNA_00002 0.71 +1.5
1,531 + CCNA_00002 0.71 +0.2
1,534 + CCNA_00002 0.72 -1.2
1,534 + CCNA_00002 0.72 -1.5
1,534 + CCNA_00002 0.72 +0.4
1,534 + CCNA_00002 0.72 -0.7
1,539 - CCNA_00002 0.72 +1.3
1,539 - CCNA_00002 0.72 -1.9
1,542 - CCNA_00002 0.73 -0.7
1,542 - CCNA_00002 0.73 -0.6
1,542 - CCNA_00002 0.73 -0.2
1,542 - CCNA_00002 0.73 -1.2
1,542 - CCNA_00002 0.73 +0.6
1,554 + CCNA_00002 0.75 +0.5
1,554 + CCNA_00002 0.75 +0.4
1,554 + CCNA_00002 0.75 +0.2
1,554 + CCNA_00002 0.75 +1.2
1,554 + CCNA_00002 0.75 -0.8
1,554 + CCNA_00002 0.75 -0.4
1,554 + CCNA_00002 0.75 +0.1
1,559 + CCNA_00002 0.76 -0.8
1,559 + CCNA_00002 0.76 +2.0
1,562 - CCNA_00002 0.76 +1.7
1,562 - CCNA_00002 0.76 -1.0
1,562 - CCNA_00002 0.76 -2.0
1,562 - CCNA_00002 0.76 +1.4
1,567 - CCNA_00002 0.77 -1.3
1,567 - CCNA_00002 0.77 -0.4
1,567 - CCNA_00002 0.77 -0.7
1,576 + CCNA_00002 0.79 +0.0
1,576 + CCNA_00002 0.79 +0.3
1,576 + CCNA_00002 0.79 +0.3
1,581 + CCNA_00002 0.80 +0.2
1,581 + CCNA_00002 0.80 -0.4
1,581 - CCNA_00002 0.80 +0.6
1,589 - CCNA_00002 0.81 +1.0
1,591 - CCNA_00002 0.81 -0.7
1,605 - CCNA_00002 0.83 -1.4
1,610 + CCNA_00002 0.84 +0.3
1,639 + CCNA_00002 0.89 -0.9
1,647 - +0.6
1,663 - +2.6
1,689 + +1.5
1,696 + -0.9
1,697 - +0.0
1,697 - -1.4
1,739 + +1.6
1,739 + -1.1
1,747 - -0.9
1,751 - -0.1
1,768 - +0.8
1,814 - CCNA_00003 0.13 +0.3
1,817 + CCNA_00003 0.14 +0.3
1,849 + CCNA_00003 0.17 -0.8
1,849 + CCNA_00003 0.17 -1.4
1,901 - CCNA_00003 0.23 +1.0
1,905 + CCNA_00003 0.24 +2.2
1,919 + CCNA_00003 0.26 -0.9
1,980 + CCNA_00003 0.33 -1.9
2,039 - CCNA_00003 0.40 -1.1
2,044 - CCNA_00003 0.40 +1.3
2,077 - CCNA_00003 0.44 -0.9

Or see this region's nucleotide sequence