Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0022 and Shew_0023 overlap by 1 nucleotides Shew_0023 and Shew_0024 are separated by 21 nucleotides Shew_0024 and Shew_0025 are separated by 272 nucleotides Shew_0025 and Shew_0026 are separated by 115 nucleotides
Shew_0022: Shew_0022 - TrkH family potassium uptake protein (RefSeq), at 25,599 to 27,056
_0022
Shew_0023: Shew_0023 - protoporphyrinogen oxidase (RefSeq), at 27,056 to 27,583
_0023
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Shew_0025: Shew_0025 - protoporphyrinogen oxidase (RefSeq), at 28,183 to 28,716
_0025
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Position (kb) 27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 27.032 kb on - strand at 27.097 kb on + strand at 27.165 kb on + strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.208 kb on + strand, within Shew_0023 at 27.420 kb on - strand, within Shew_0023 at 27.540 kb on + strand at 27.556 kb on - strand at 27.567 kb on + strand at 27.594 kb on - strand at 27.671 kb on + strand, within Shew_0024 at 27.851 kb on - strand, within Shew_0024 at 28.301 kb on + strand, within Shew_0025 at 28.365 kb on - strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.442 kb on + strand, within Shew_0025 at 28.700 kb on - strand at 28.750 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 27,032 - +1.5 27,097 + +0.1 27,165 + Shew_0023 0.21 -1.0 27,173 - Shew_0023 0.22 +0.0 27,173 - Shew_0023 0.22 -0.4 27,208 + Shew_0023 0.29 -0.3 27,420 - Shew_0023 0.69 +0.6 27,540 + +0.9 27,556 - +0.2 27,567 + -0.1 27,594 - -0.2 27,671 + Shew_0024 0.22 -1.5 27,851 - Shew_0024 0.80 -1.5 28,301 + Shew_0025 0.22 +0.5 28,365 - Shew_0025 0.34 +0.2 28,401 + Shew_0025 0.41 +0.1 28,401 + Shew_0025 0.41 -2.1 28,401 + Shew_0025 0.41 +0.0 28,442 + Shew_0025 0.49 +0.5 28,700 - +0.0 28,750 + -0.3
Or see this region's nucleotide sequence