Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0825 and Dshi_0826 are separated by 180 nucleotides Dshi_0826 and Dshi_0827 are separated by 173 nucleotides Dshi_0827 and Dshi_0828 overlap by 4 nucleotides
Dshi_0825: Dshi_0825 - AMP-dependent synthetase and ligase (RefSeq), at 830,837 to 832,729
_0825
Dshi_0826: Dshi_0826 - malic protein NAD-binding (RefSeq), at 832,910 to 835,189
_0826
Dshi_0827: Dshi_0827 - cytidine deaminase (RefSeq), at 835,363 to 835,755
_0827
Dshi_0828: Dshi_0828 - pyrimidine-nucleoside phosphorylase (RefSeq), at 835,752 to 837,059
_0828
Position (kb)
832
833
834
835
836 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 831.916 kb on + strand, within Dshi_0825 at 831.917 kb on - strand, within Dshi_0825 at 831.950 kb on - strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.997 kb on + strand, within Dshi_0825 at 832.022 kb on - strand, within Dshi_0825 at 832.046 kb on - strand, within Dshi_0825 at 832.145 kb on + strand, within Dshi_0825 at 832.193 kb on + strand, within Dshi_0825 at 832.213 kb on - strand, within Dshi_0825 at 832.579 kb on - strand at 832.614 kb on + strand at 832.707 kb on + strand at 832.707 kb on + strand at 832.715 kb on - strand at 832.715 kb on - strand at 832.715 kb on - strand at 832.717 kb on + strand at 832.765 kb on + strand at 832.949 kb on + strand at 832.993 kb on - strand at 833.070 kb on + strand at 833.152 kb on + strand, within Dshi_0826 at 833.188 kb on - strand, within Dshi_0826 at 833.233 kb on - strand, within Dshi_0826 at 833.274 kb on - strand, within Dshi_0826 at 833.291 kb on - strand, within Dshi_0826 at 833.313 kb on - strand, within Dshi_0826 at 833.383 kb on + strand, within Dshi_0826 at 833.386 kb on + strand, within Dshi_0826 at 833.457 kb on - strand, within Dshi_0826 at 833.658 kb on + strand, within Dshi_0826 at 833.775 kb on - strand, within Dshi_0826 at 833.776 kb on + strand, within Dshi_0826 at 833.921 kb on - strand, within Dshi_0826 at 834.124 kb on - strand, within Dshi_0826 at 834.268 kb on + strand, within Dshi_0826 at 834.300 kb on - strand, within Dshi_0826 at 834.323 kb on - strand, within Dshi_0826 at 834.449 kb on + strand, within Dshi_0826 at 834.734 kb on - strand, within Dshi_0826 at 834.767 kb on - strand, within Dshi_0826 at 834.817 kb on - strand, within Dshi_0826 at 834.817 kb on - strand, within Dshi_0826 at 834.889 kb on + strand, within Dshi_0826 at 834.902 kb on - strand, within Dshi_0826 at 834.921 kb on - strand, within Dshi_0826 at 834.999 kb on - strand at 835.111 kb on + strand at 835.111 kb on + strand at 835.344 kb on - strand at 835.379 kb on - strand at 835.418 kb on - strand, within Dshi_0827 at 835.459 kb on - strand, within Dshi_0827 at 835.469 kb on - strand, within Dshi_0827 at 835.597 kb on - strand, within Dshi_0827 at 835.622 kb on + strand, within Dshi_0827 at 836.098 kb on - strand, within Dshi_0828
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 831,916 + Dshi_0825 0.57 -0.4 831,917 - Dshi_0825 0.57 -0.8 831,950 - Dshi_0825 0.59 -1.4 831,987 + Dshi_0825 0.61 +0.6 831,987 + Dshi_0825 0.61 +0.3 831,987 + Dshi_0825 0.61 -0.6 831,987 + Dshi_0825 0.61 +0.4 831,995 - Dshi_0825 0.61 -0.5 831,995 - Dshi_0825 0.61 +1.9 831,995 - Dshi_0825 0.61 -0.6 831,995 - Dshi_0825 0.61 +0.4 831,995 - Dshi_0825 0.61 -0.1 831,997 + Dshi_0825 0.61 -1.0 832,022 - Dshi_0825 0.63 -1.1 832,046 - Dshi_0825 0.64 +0.5 832,145 + Dshi_0825 0.69 +0.6 832,193 + Dshi_0825 0.72 -0.7 832,213 - Dshi_0825 0.73 -0.5 832,579 - +0.5 832,614 + +0.0 832,707 + -1.2 832,707 + -0.2 832,715 - -0.2 832,715 - +1.2 832,715 - -0.9 832,717 + +0.7 832,765 + -0.5 832,949 + -0.6 832,993 - -0.2 833,070 + -1.0 833,152 + Dshi_0826 0.11 -1.8 833,188 - Dshi_0826 0.12 -1.1 833,233 - Dshi_0826 0.14 -1.7 833,274 - Dshi_0826 0.16 -1.0 833,291 - Dshi_0826 0.17 +0.0 833,313 - Dshi_0826 0.18 -0.2 833,383 + Dshi_0826 0.21 -0.6 833,386 + Dshi_0826 0.21 -0.6 833,457 - Dshi_0826 0.24 -1.1 833,658 + Dshi_0826 0.33 -0.4 833,775 - Dshi_0826 0.38 +0.0 833,776 + Dshi_0826 0.38 -0.7 833,921 - Dshi_0826 0.44 -1.2 834,124 - Dshi_0826 0.53 -0.5 834,268 + Dshi_0826 0.60 +0.0 834,300 - Dshi_0826 0.61 -0.4 834,323 - Dshi_0826 0.62 -1.1 834,449 + Dshi_0826 0.68 -1.0 834,734 - Dshi_0826 0.80 -0.4 834,767 - Dshi_0826 0.81 -2.6 834,817 - Dshi_0826 0.84 -1.4 834,817 - Dshi_0826 0.84 -0.7 834,889 + Dshi_0826 0.87 -1.0 834,902 - Dshi_0826 0.87 -1.0 834,921 - Dshi_0826 0.88 -1.1 834,999 - -0.7 835,111 + -1.5 835,111 + -0.2 835,344 - -0.3 835,379 - +0.1 835,418 - Dshi_0827 0.14 -0.5 835,459 - Dshi_0827 0.24 +0.8 835,469 - Dshi_0827 0.27 +0.8 835,597 - Dshi_0827 0.60 +0.0 835,622 + Dshi_0827 0.66 +0.4 836,098 - Dshi_0828 0.26 +0.5
Or see this region's nucleotide sequence