Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0176 and Dshi_0177 are separated by 60 nucleotides Dshi_0177 and Dshi_0178 are separated by 3 nucleotides Dshi_0178 and Dshi_0179 are separated by 73 nucleotides Dshi_0179 and Dshi_0180 are separated by 72 nucleotides
Dshi_0176: Dshi_0176 - precorrin-4 C11-methyltransferase (RefSeq), at 180,607 to 181,383
_0176
Dshi_0177: Dshi_0177 - hypothetical protein (RefSeq), at 181,444 to 181,797
_0177
Dshi_0178: Dshi_0178 - aminotransferase class I and II (RefSeq), at 181,801 to 182,973
_0178
Dshi_0179: Dshi_0179 - peptide deformylase (RefSeq), at 183,047 to 183,610
_0179
Dshi_0180: Dshi_0180 - peptide deformylase (RefSeq), at 183,683 to 184,189
_0180
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2
3 at 181.416 kb on + strand at 181.696 kb on - strand, within Dshi_0177 at 181.908 kb on + strand at 181.916 kb on - strand at 181.979 kb on - strand, within Dshi_0178 at 182.002 kb on + strand, within Dshi_0178 at 182.002 kb on + strand, within Dshi_0178 at 182.351 kb on - strand, within Dshi_0178 at 182.361 kb on - strand, within Dshi_0178 at 182.438 kb on + strand, within Dshi_0178 at 182.438 kb on + strand, within Dshi_0178 at 182.530 kb on + strand, within Dshi_0178 at 182.539 kb on - strand at 182.539 kb on - strand, within Dshi_0178 at 182.563 kb on + strand, within Dshi_0178 at 182.609 kb on + strand, within Dshi_0178 at 182.668 kb on + strand, within Dshi_0178 at 182.730 kb on - strand, within Dshi_0178 at 182.770 kb on + strand, within Dshi_0178 at 182.874 kb on + strand at 182.939 kb on + strand at 182.947 kb on - strand at 182.968 kb on + strand at 182.976 kb on - strand at 182.988 kb on - strand at 183.760 kb on + strand, within Dshi_0180 at 183.827 kb on + strand, within Dshi_0180 at 183.959 kb on - strand, within Dshi_0180
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 181,416 + +0.6 181,696 - Dshi_0177 0.71 +0.5 181,908 + -0.3 181,916 - +0.7 181,979 - Dshi_0178 0.15 +0.8 182,002 + Dshi_0178 0.17 -0.3 182,002 + Dshi_0178 0.17 +0.1 182,351 - Dshi_0178 0.47 +0.6 182,361 - Dshi_0178 0.48 +0.3 182,438 + Dshi_0178 0.54 +0.4 182,438 + Dshi_0178 0.54 +3.3 182,530 + Dshi_0178 0.62 -0.9 182,539 - -0.5 182,539 - Dshi_0178 0.63 +0.3 182,563 + Dshi_0178 0.65 +0.5 182,609 + Dshi_0178 0.69 +0.1 182,668 + Dshi_0178 0.74 +0.8 182,730 - Dshi_0178 0.79 -0.2 182,770 + Dshi_0178 0.83 -0.5 182,874 + +0.1 182,939 + +1.4 182,947 - +0.1 182,968 + -0.3 182,976 - +1.5 182,988 - +0.1 183,760 + Dshi_0180 0.15 +1.1 183,827 + Dshi_0180 0.28 -0.2 183,959 - Dshi_0180 0.54 +0.3
Or see this region's nucleotide sequence