Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0065 and Dshi_0066 are separated by 115 nucleotides Dshi_0066 and Dshi_0067 overlap by 4 nucleotides Dshi_0067 and Dshi_0068 are separated by 4 nucleotides
Dshi_0065: Dshi_0065 - hypothetical protein (RefSeq), at 65,978 to 67,468
_0065
Dshi_0066: Dshi_0066 - short-chain dehydrogenase/reductase SDR (RefSeq), at 67,584 to 68,342
_0066
Dshi_0067: Dshi_0067 - Enoyl-CoA hydratase/isomerase (RefSeq), at 68,339 to 69,085
_0067
Dshi_0068: Dshi_0068 - lipoprotein, putative (RefSeq), at 69,090 to 69,674
_0068
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 66.673 kb on - strand, within Dshi_0065 at 66.817 kb on - strand, within Dshi_0065 at 66.990 kb on - strand, within Dshi_0065 at 67.067 kb on + strand, within Dshi_0065 at 67.077 kb on + strand, within Dshi_0065 at 67.080 kb on - strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.126 kb on + strand at 67.135 kb on - strand, within Dshi_0065 at 67.242 kb on - strand, within Dshi_0065 at 67.365 kb on + strand at 67.366 kb on + strand at 67.373 kb on - strand at 67.395 kb on - strand at 67.406 kb on - strand at 67.417 kb on - strand at 67.456 kb on + strand at 67.474 kb on - strand at 67.527 kb on - strand at 67.531 kb on + strand at 67.531 kb on + strand at 67.537 kb on - strand at 67.553 kb on - strand at 67.563 kb on + strand at 67.578 kb on - strand at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.810 kb on - strand at 67.810 kb on - strand, within Dshi_0066 at 67.851 kb on - strand, within Dshi_0066 at 67.920 kb on - strand, within Dshi_0066 at 67.931 kb on + strand, within Dshi_0066 at 68.208 kb on + strand, within Dshi_0066 at 68.251 kb on - strand, within Dshi_0066 at 68.393 kb on + strand at 68.398 kb on + strand at 68.533 kb on + strand, within Dshi_0067 at 68.585 kb on + strand, within Dshi_0067 at 68.594 kb on - strand, within Dshi_0067 at 68.595 kb on + strand, within Dshi_0067 at 68.600 kb on + strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.772 kb on - strand, within Dshi_0067 at 68.935 kb on + strand, within Dshi_0067
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 66,673 - Dshi_0065 0.47 +1.1 66,817 - Dshi_0065 0.56 -0.4 66,990 - Dshi_0065 0.68 -0.2 67,067 + Dshi_0065 0.73 -0.6 67,077 + Dshi_0065 0.74 +0.1 67,080 - Dshi_0065 0.74 +0.1 67,104 + Dshi_0065 0.76 +2.2 67,104 + Dshi_0065 0.76 -1.1 67,126 + +0.1 67,135 - Dshi_0065 0.78 -0.8 67,242 - Dshi_0065 0.85 -2.3 67,365 + -0.3 67,366 + -0.9 67,373 - +0.7 67,395 - +0.4 67,406 - -0.9 67,417 - +0.5 67,456 + -0.8 67,474 - -0.7 67,527 - -1.4 67,531 + +0.1 67,531 + +0.7 67,537 - +0.5 67,553 - -1.7 67,563 + -0.1 67,578 - -0.0 67,748 + Dshi_0066 0.22 +0.4 67,748 + Dshi_0066 0.22 -2.3 67,748 + Dshi_0066 0.22 +0.3 67,810 - +1.1 67,810 - Dshi_0066 0.30 +1.3 67,851 - Dshi_0066 0.35 -0.2 67,920 - Dshi_0066 0.44 -0.6 67,931 + Dshi_0066 0.46 +0.2 68,208 + Dshi_0066 0.82 -0.5 68,251 - Dshi_0066 0.88 -0.2 68,393 + +0.1 68,398 + +0.1 68,533 + Dshi_0067 0.26 +0.7 68,585 + Dshi_0067 0.33 -0.4 68,594 - Dshi_0067 0.34 +0.1 68,595 + Dshi_0067 0.34 +0.8 68,600 + Dshi_0067 0.35 +1.1 68,603 - Dshi_0067 0.35 +0.1 68,603 - Dshi_0067 0.35 +0.1 68,772 - Dshi_0067 0.58 +1.1 68,935 + Dshi_0067 0.80 +0.1
Or see this region's nucleotide sequence