Experiment: PYE with benzoic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01538 and CCNA_03952 are separated by 116 nucleotides CCNA_03952 and CCNA_01539 overlap by 47 nucleotides CCNA_01539 and CCNA_01541 are separated by 125 nucleotides
CCNA_01538: CCNA_01538 - pyruvate phosphate dikinase, at 1,646,212 to 1,648,902
_01538
CCNA_03952: CCNA_03952 - hypothetical protein, at 1,649,019 to 1,649,504
_03952
CCNA_01539: CCNA_01539 - pirin family protein, at 1,649,458 to 1,650,156
_01539
CCNA_01541: CCNA_01541 - LysR-family transcriptional regulator, at 1,650,282 to 1,651,178
_01541
Position (kb)
1649
1650 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1648.879 kb on + strand at 1648.933 kb on + strand at 1648.933 kb on + strand at 1648.942 kb on - strand at 1648.945 kb on + strand at 1648.966 kb on + strand at 1649.074 kb on - strand, within CCNA_03952 at 1649.074 kb on - strand, within CCNA_03952 at 1649.117 kb on + strand, within CCNA_03952 at 1649.203 kb on + strand, within CCNA_03952 at 1649.211 kb on - strand, within CCNA_03952 at 1649.213 kb on + strand, within CCNA_03952 at 1649.216 kb on - strand, within CCNA_03952 at 1649.221 kb on - strand, within CCNA_03952 at 1649.234 kb on - strand, within CCNA_03952 at 1649.262 kb on + strand, within CCNA_03952 at 1649.282 kb on + strand, within CCNA_03952 at 1649.282 kb on + strand, within CCNA_03952 at 1649.282 kb on + strand, within CCNA_03952 at 1649.290 kb on - strand, within CCNA_03952 at 1649.290 kb on - strand, within CCNA_03952 at 1649.301 kb on - strand, within CCNA_03952 at 1649.398 kb on - strand, within CCNA_03952 at 1649.455 kb on + strand, within CCNA_03952 at 1649.455 kb on + strand, within CCNA_03952 at 1649.463 kb on - strand at 1649.463 kb on - strand at 1649.504 kb on - strand at 1649.507 kb on - strand at 1649.517 kb on - strand at 1649.622 kb on - strand, within CCNA_01539 at 1649.627 kb on - strand, within CCNA_01539 at 1649.644 kb on + strand, within CCNA_01539 at 1649.680 kb on + strand, within CCNA_01539 at 1649.731 kb on - strand, within CCNA_01539 at 1649.747 kb on + strand, within CCNA_01539 at 1649.747 kb on + strand, within CCNA_01539 at 1649.747 kb on + strand, within CCNA_01539 at 1649.755 kb on - strand, within CCNA_01539 at 1649.767 kb on - strand, within CCNA_01539 at 1649.801 kb on - strand, within CCNA_01539 at 1649.804 kb on + strand, within CCNA_01539 at 1649.812 kb on - strand, within CCNA_01539 at 1649.840 kb on + strand, within CCNA_01539 at 1649.850 kb on - strand, within CCNA_01539 at 1649.870 kb on - strand, within CCNA_01539 at 1649.871 kb on - strand, within CCNA_01539 at 1649.881 kb on + strand, within CCNA_01539 at 1649.882 kb on + strand, within CCNA_01539 at 1649.916 kb on + strand, within CCNA_01539 at 1649.924 kb on - strand, within CCNA_01539 at 1649.955 kb on + strand, within CCNA_01539 at 1649.975 kb on - strand, within CCNA_01539 at 1650.026 kb on + strand, within CCNA_01539 at 1650.106 kb on - strand at 1650.166 kb on + strand at 1650.282 kb on - strand at 1650.346 kb on - strand at 1650.363 kb on + strand at 1650.371 kb on - strand at 1650.371 kb on - strand at 1650.374 kb on + strand, within CCNA_01541 at 1650.382 kb on + strand, within CCNA_01541 at 1650.389 kb on + strand, within CCNA_01541 at 1650.411 kb on + strand, within CCNA_01541 at 1650.424 kb on + strand, within CCNA_01541 at 1650.424 kb on + strand, within CCNA_01541 at 1650.442 kb on - strand, within CCNA_01541 at 1650.454 kb on + strand, within CCNA_01541 at 1650.459 kb on + strand, within CCNA_01541 at 1650.459 kb on + strand, within CCNA_01541 at 1650.459 kb on + strand, within CCNA_01541 at 1650.462 kb on - strand, within CCNA_01541 at 1650.462 kb on - strand, within CCNA_01541 at 1650.464 kb on + strand, within CCNA_01541 at 1650.485 kb on + strand, within CCNA_01541
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with benzoic 0.2 mg/ml remove 1,648,879 + +3.4 1,648,933 + +3.4 1,648,933 + +3.0 1,648,942 - +0.2 1,648,945 + +2.8 1,648,966 + +2.2 1,649,074 - CCNA_03952 0.11 -0.6 1,649,074 - CCNA_03952 0.11 +0.5 1,649,117 + CCNA_03952 0.20 +2.2 1,649,203 + CCNA_03952 0.38 +2.2 1,649,211 - CCNA_03952 0.40 +2.5 1,649,213 + CCNA_03952 0.40 +2.5 1,649,216 - CCNA_03952 0.41 +1.0 1,649,221 - CCNA_03952 0.42 +0.7 1,649,234 - CCNA_03952 0.44 +0.5 1,649,262 + CCNA_03952 0.50 +3.1 1,649,282 + CCNA_03952 0.54 +2.3 1,649,282 + CCNA_03952 0.54 +2.5 1,649,282 + CCNA_03952 0.54 +2.8 1,649,290 - CCNA_03952 0.56 -2.3 1,649,290 - CCNA_03952 0.56 -0.3 1,649,301 - CCNA_03952 0.58 +0.2 1,649,398 - CCNA_03952 0.78 +1.2 1,649,455 + CCNA_03952 0.90 +0.9 1,649,455 + CCNA_03952 0.90 +1.1 1,649,463 - +1.1 1,649,463 - +1.8 1,649,504 - +0.7 1,649,507 - +0.7 1,649,517 - +1.4 1,649,622 - CCNA_01539 0.23 +0.9 1,649,627 - CCNA_01539 0.24 +0.5 1,649,644 + CCNA_01539 0.27 +1.5 1,649,680 + CCNA_01539 0.32 +0.5 1,649,731 - CCNA_01539 0.39 -1.3 1,649,747 + CCNA_01539 0.41 -0.1 1,649,747 + CCNA_01539 0.41 +0.0 1,649,747 + CCNA_01539 0.41 -0.8 1,649,755 - CCNA_01539 0.42 +0.5 1,649,767 - CCNA_01539 0.44 +0.1 1,649,801 - CCNA_01539 0.49 -0.6 1,649,804 + CCNA_01539 0.49 +0.9 1,649,812 - CCNA_01539 0.51 +0.0 1,649,840 + CCNA_01539 0.55 -1.6 1,649,850 - CCNA_01539 0.56 -0.6 1,649,870 - CCNA_01539 0.59 -0.1 1,649,871 - CCNA_01539 0.59 +0.6 1,649,881 + CCNA_01539 0.61 -1.5 1,649,882 + CCNA_01539 0.61 -1.9 1,649,916 + CCNA_01539 0.66 +2.1 1,649,924 - CCNA_01539 0.67 -0.2 1,649,955 + CCNA_01539 0.71 +1.0 1,649,975 - CCNA_01539 0.74 +1.2 1,650,026 + CCNA_01539 0.81 +0.5 1,650,106 - +0.5 1,650,166 + +0.3 1,650,282 - -0.4 1,650,346 - +0.2 1,650,363 + +2.5 1,650,371 - +1.5 1,650,371 - +1.9 1,650,374 + CCNA_01541 0.10 +1.5 1,650,382 + CCNA_01541 0.11 +2.1 1,650,389 + CCNA_01541 0.12 +1.9 1,650,411 + CCNA_01541 0.14 +2.3 1,650,424 + CCNA_01541 0.16 +1.6 1,650,424 + CCNA_01541 0.16 +1.6 1,650,442 - CCNA_01541 0.18 -0.1 1,650,454 + CCNA_01541 0.19 +2.0 1,650,459 + CCNA_01541 0.20 +2.0 1,650,459 + CCNA_01541 0.20 +2.3 1,650,459 + CCNA_01541 0.20 +2.5 1,650,462 - CCNA_01541 0.20 +1.5 1,650,462 - CCNA_01541 0.20 +1.2 1,650,464 + CCNA_01541 0.20 +2.5 1,650,485 + CCNA_01541 0.23 +2.7
Or see this region's nucleotide sequence