Experiment: PYE with benzoic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03802 and CCNA_03803 are separated by 130 nucleotides CCNA_03803 and CCNA_03804 are separated by 111 nucleotides CCNA_03804 and CCNA_03805 overlap by 32 nucleotides
CCNA_03802: CCNA_03802 - serine protease, peptidase family S9A, at 3,966,471 to 3,968,627
_03802
CCNA_03803: CCNA_03803 - cellulose biosynthesis protein CelD, at 3,968,758 to 3,969,843
_03803
CCNA_03804: CCNA_03804 - hypothetical protein, at 3,969,955 to 3,970,287
_03804
CCNA_03805: CCNA_03805 - PadR-family transcriptional regulator, at 3,970,256 to 3,970,834
_03805
Position (kb)
3968
3969
3970 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3967.758 kb on - strand, within CCNA_03802 at 3967.765 kb on + strand, within CCNA_03802 at 3967.765 kb on + strand, within CCNA_03802 at 3967.795 kb on + strand, within CCNA_03802 at 3967.803 kb on - strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.805 kb on + strand, within CCNA_03802 at 3967.813 kb on - strand, within CCNA_03802 at 3967.813 kb on - strand, within CCNA_03802 at 3967.813 kb on - strand, within CCNA_03802 at 3967.813 kb on - strand, within CCNA_03802 at 3967.813 kb on - strand, within CCNA_03802 at 3967.826 kb on + strand, within CCNA_03802 at 3967.848 kb on + strand, within CCNA_03802 at 3967.864 kb on - strand, within CCNA_03802 at 3967.870 kb on + strand, within CCNA_03802 at 3967.870 kb on + strand, within CCNA_03802 at 3967.870 kb on + strand, within CCNA_03802 at 3967.878 kb on - strand, within CCNA_03802 at 3967.879 kb on + strand, within CCNA_03802 at 3967.907 kb on + strand, within CCNA_03802 at 3967.910 kb on - strand, within CCNA_03802 at 3967.915 kb on - strand, within CCNA_03802 at 3967.915 kb on - strand, within CCNA_03802 at 3967.915 kb on - strand, within CCNA_03802 at 3967.915 kb on - strand, within CCNA_03802 at 3967.941 kb on + strand, within CCNA_03802 at 3967.949 kb on - strand, within CCNA_03802 at 3967.954 kb on - strand, within CCNA_03802 at 3967.954 kb on - strand, within CCNA_03802 at 3967.954 kb on - strand, within CCNA_03802 at 3967.982 kb on - strand, within CCNA_03802 at 3968.009 kb on + strand, within CCNA_03802 at 3968.017 kb on - strand, within CCNA_03802 at 3968.017 kb on - strand, within CCNA_03802 at 3968.076 kb on + strand, within CCNA_03802 at 3968.084 kb on - strand, within CCNA_03802 at 3968.092 kb on + strand, within CCNA_03802 at 3968.092 kb on + strand, within CCNA_03802 at 3968.092 kb on + strand, within CCNA_03802 at 3968.100 kb on - strand, within CCNA_03802 at 3968.115 kb on + strand, within CCNA_03802 at 3968.126 kb on + strand, within CCNA_03802 at 3968.145 kb on + strand, within CCNA_03802 at 3968.153 kb on - strand, within CCNA_03802 at 3968.162 kb on + strand, within CCNA_03802 at 3968.211 kb on + strand, within CCNA_03802 at 3968.226 kb on + strand, within CCNA_03802 at 3968.227 kb on + strand, within CCNA_03802 at 3968.244 kb on + strand, within CCNA_03802 at 3968.249 kb on + strand, within CCNA_03802 at 3968.249 kb on + strand, within CCNA_03802 at 3968.257 kb on - strand, within CCNA_03802 at 3968.298 kb on + strand, within CCNA_03802 at 3968.303 kb on + strand, within CCNA_03802 at 3968.317 kb on + strand, within CCNA_03802 at 3968.321 kb on - strand, within CCNA_03802 at 3968.386 kb on - strand, within CCNA_03802 at 3968.396 kb on + strand, within CCNA_03802 at 3968.416 kb on + strand at 3968.416 kb on + strand at 3968.416 kb on + strand at 3968.424 kb on + strand at 3968.424 kb on - strand at 3968.424 kb on - strand at 3968.475 kb on + strand at 3968.499 kb on + strand at 3968.504 kb on + strand at 3968.507 kb on - strand at 3968.509 kb on + strand at 3968.512 kb on - strand at 3968.514 kb on + strand at 3968.549 kb on + strand at 3968.564 kb on + strand at 3968.564 kb on + strand at 3968.626 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.646 kb on + strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.654 kb on - strand at 3968.656 kb on - strand at 3968.666 kb on + strand at 3968.666 kb on + strand at 3968.674 kb on - strand at 3968.674 kb on - strand at 3968.698 kb on + strand at 3968.706 kb on - strand at 3968.719 kb on + strand at 3968.755 kb on + strand at 3968.820 kb on + strand at 3968.831 kb on + strand at 3968.863 kb on + strand at 3968.867 kb on + strand, within CCNA_03803 at 3968.875 kb on - strand, within CCNA_03803 at 3968.903 kb on - strand, within CCNA_03803 at 3968.913 kb on + strand, within CCNA_03803 at 3968.913 kb on + strand, within CCNA_03803 at 3968.913 kb on + strand, within CCNA_03803 at 3968.986 kb on - strand, within CCNA_03803 at 3968.986 kb on - strand, within CCNA_03803 at 3968.988 kb on + strand, within CCNA_03803 at 3968.990 kb on - strand, within CCNA_03803 at 3968.992 kb on - strand, within CCNA_03803 at 3969.004 kb on + strand, within CCNA_03803 at 3969.020 kb on + strand, within CCNA_03803 at 3969.024 kb on + strand, within CCNA_03803 at 3969.025 kb on + strand, within CCNA_03803 at 3969.025 kb on + strand, within CCNA_03803 at 3969.033 kb on - strand, within CCNA_03803 at 3969.088 kb on + strand, within CCNA_03803 at 3969.142 kb on - strand, within CCNA_03803 at 3969.167 kb on - strand, within CCNA_03803 at 3969.207 kb on + strand, within CCNA_03803 at 3969.207 kb on + strand, within CCNA_03803 at 3969.207 kb on + strand, within CCNA_03803 at 3969.207 kb on + strand, within CCNA_03803 at 3969.215 kb on - strand, within CCNA_03803 at 3969.263 kb on - strand, within CCNA_03803 at 3969.277 kb on + strand, within CCNA_03803 at 3969.378 kb on + strand, within CCNA_03803 at 3969.378 kb on + strand, within CCNA_03803 at 3969.378 kb on + strand at 3969.378 kb on + strand, within CCNA_03803 at 3969.378 kb on + strand, within CCNA_03803 at 3969.378 kb on + strand, within CCNA_03803 at 3969.386 kb on - strand, within CCNA_03803 at 3969.386 kb on - strand, within CCNA_03803 at 3969.386 kb on - strand, within CCNA_03803 at 3969.402 kb on + strand, within CCNA_03803 at 3969.450 kb on + strand, within CCNA_03803 at 3969.450 kb on + strand, within CCNA_03803 at 3969.450 kb on + strand, within CCNA_03803 at 3969.458 kb on - strand, within CCNA_03803 at 3969.458 kb on - strand, within CCNA_03803 at 3969.458 kb on - strand, within CCNA_03803 at 3969.491 kb on + strand, within CCNA_03803 at 3969.517 kb on + strand, within CCNA_03803 at 3969.517 kb on + strand, within CCNA_03803 at 3969.517 kb on + strand, within CCNA_03803 at 3969.517 kb on + strand, within CCNA_03803 at 3969.525 kb on - strand, within CCNA_03803 at 3969.558 kb on - strand, within CCNA_03803 at 3969.579 kb on + strand, within CCNA_03803 at 3969.579 kb on + strand, within CCNA_03803 at 3969.579 kb on + strand, within CCNA_03803 at 3969.597 kb on - strand, within CCNA_03803 at 3969.607 kb on - strand, within CCNA_03803 at 3969.613 kb on + strand, within CCNA_03803 at 3969.666 kb on + strand, within CCNA_03803 at 3969.673 kb on + strand, within CCNA_03803 at 3969.688 kb on + strand, within CCNA_03803 at 3969.688 kb on + strand, within CCNA_03803 at 3969.747 kb on - strand at 3969.747 kb on - strand at 3969.772 kb on - strand at 3969.774 kb on + strand at 3969.791 kb on - strand at 3969.793 kb on + strand at 3969.803 kb on - strand at 3969.837 kb on + strand at 3969.837 kb on + strand at 3969.845 kb on - strand at 3969.845 kb on - strand at 3969.906 kb on + strand at 3970.009 kb on + strand, within CCNA_03804 at 3970.009 kb on + strand, within CCNA_03804 at 3970.032 kb on + strand, within CCNA_03804 at 3970.042 kb on + strand, within CCNA_03804 at 3970.085 kb on - strand at 3970.085 kb on - strand, within CCNA_03804 at 3970.085 kb on - strand, within CCNA_03804 at 3970.085 kb on - strand, within CCNA_03804 at 3970.085 kb on - strand, within CCNA_03804 at 3970.121 kb on - strand, within CCNA_03804 at 3970.166 kb on - strand, within CCNA_03804 at 3970.167 kb on + strand, within CCNA_03804 at 3970.172 kb on + strand, within CCNA_03804 at 3970.172 kb on + strand, within CCNA_03804 at 3970.172 kb on + strand, within CCNA_03804 at 3970.180 kb on - strand, within CCNA_03804 at 3970.180 kb on - strand, within CCNA_03804 at 3970.180 kb on - strand, within CCNA_03804 at 3970.191 kb on + strand, within CCNA_03804 at 3970.208 kb on + strand, within CCNA_03804 at 3970.254 kb on + strand at 3970.254 kb on + strand at 3970.254 kb on + strand at 3970.294 kb on - strand at 3970.321 kb on - strand, within CCNA_03805 at 3970.328 kb on - strand, within CCNA_03805 at 3970.328 kb on - strand, within CCNA_03805 at 3970.367 kb on - strand, within CCNA_03805 at 3970.367 kb on - strand, within CCNA_03805 at 3970.369 kb on - strand, within CCNA_03805 at 3970.388 kb on - strand, within CCNA_03805 at 3970.417 kb on - strand, within CCNA_03805 at 3970.417 kb on - strand, within CCNA_03805 at 3970.417 kb on - strand, within CCNA_03805 at 3970.439 kb on - strand, within CCNA_03805 at 3970.441 kb on - strand, within CCNA_03805 at 3970.464 kb on - strand, within CCNA_03805 at 3970.483 kb on - strand, within CCNA_03805 at 3970.505 kb on - strand, within CCNA_03805 at 3970.505 kb on - strand, within CCNA_03805 at 3970.544 kb on - strand, within CCNA_03805 at 3970.544 kb on - strand, within CCNA_03805 at 3970.567 kb on - strand, within CCNA_03805 at 3970.572 kb on - strand, within CCNA_03805 at 3970.572 kb on - strand, within CCNA_03805 at 3970.631 kb on - strand, within CCNA_03805 at 3970.690 kb on - strand, within CCNA_03805 at 3970.690 kb on - strand, within CCNA_03805 at 3970.737 kb on + strand, within CCNA_03805 at 3970.745 kb on - strand, within CCNA_03805
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with benzoic 0.2 mg/ml remove 3,967,758 - CCNA_03802 0.60 +0.5 3,967,765 + CCNA_03802 0.60 +0.7 3,967,765 + CCNA_03802 0.60 +1.0 3,967,795 + CCNA_03802 0.61 -2.2 3,967,803 - CCNA_03802 0.62 +1.9 3,967,805 + CCNA_03802 0.62 +1.0 3,967,805 + CCNA_03802 0.62 +0.8 3,967,805 + CCNA_03802 0.62 +1.0 3,967,805 + CCNA_03802 0.62 +2.5 3,967,805 + CCNA_03802 0.62 +1.1 3,967,805 + CCNA_03802 0.62 +1.5 3,967,813 - CCNA_03802 0.62 +1.1 3,967,813 - CCNA_03802 0.62 +1.1 3,967,813 - CCNA_03802 0.62 +0.7 3,967,813 - CCNA_03802 0.62 +0.1 3,967,813 - CCNA_03802 0.62 -2.2 3,967,826 + CCNA_03802 0.63 -0.1 3,967,848 + CCNA_03802 0.64 +2.1 3,967,864 - CCNA_03802 0.65 +0.1 3,967,870 + CCNA_03802 0.65 -1.5 3,967,870 + CCNA_03802 0.65 +1.7 3,967,870 + CCNA_03802 0.65 +1.1 3,967,878 - CCNA_03802 0.65 +1.9 3,967,879 + CCNA_03802 0.65 +0.7 3,967,907 + CCNA_03802 0.67 +1.1 3,967,910 - CCNA_03802 0.67 -1.2 3,967,915 - CCNA_03802 0.67 +0.5 3,967,915 - CCNA_03802 0.67 -0.7 3,967,915 - CCNA_03802 0.67 +0.3 3,967,915 - CCNA_03802 0.67 +2.1 3,967,941 + CCNA_03802 0.68 +1.2 3,967,949 - CCNA_03802 0.69 -0.3 3,967,954 - CCNA_03802 0.69 +0.5 3,967,954 - CCNA_03802 0.69 +0.5 3,967,954 - CCNA_03802 0.69 -1.0 3,967,982 - CCNA_03802 0.70 +1.7 3,968,009 + CCNA_03802 0.71 +0.5 3,968,017 - CCNA_03802 0.72 -1.3 3,968,017 - CCNA_03802 0.72 -1.4 3,968,076 + CCNA_03802 0.74 -1.2 3,968,084 - CCNA_03802 0.75 -0.5 3,968,092 + CCNA_03802 0.75 +1.2 3,968,092 + CCNA_03802 0.75 +0.7 3,968,092 + CCNA_03802 0.75 -2.0 3,968,100 - CCNA_03802 0.76 +1.6 3,968,115 + CCNA_03802 0.76 +0.5 3,968,126 + CCNA_03802 0.77 -0.2 3,968,145 + CCNA_03802 0.78 +2.3 3,968,153 - CCNA_03802 0.78 +1.1 3,968,162 + CCNA_03802 0.78 +3.1 3,968,211 + CCNA_03802 0.81 +0.8 3,968,226 + CCNA_03802 0.81 +0.7 3,968,227 + CCNA_03802 0.81 +0.8 3,968,244 + CCNA_03802 0.82 +1.9 3,968,249 + CCNA_03802 0.82 +0.9 3,968,249 + CCNA_03802 0.82 +0.6 3,968,257 - CCNA_03802 0.83 +0.1 3,968,298 + CCNA_03802 0.85 +1.4 3,968,303 + CCNA_03802 0.85 +0.5 3,968,317 + CCNA_03802 0.86 +0.5 3,968,321 - CCNA_03802 0.86 +0.1 3,968,386 - CCNA_03802 0.89 -0.4 3,968,396 + CCNA_03802 0.89 +1.3 3,968,416 + +0.1 3,968,416 + +1.0 3,968,416 + +1.6 3,968,424 + +0.8 3,968,424 - -2.1 3,968,424 - -2.0 3,968,475 + +2.1 3,968,499 + +1.5 3,968,504 + +0.7 3,968,507 - +0.5 3,968,509 + +1.1 3,968,512 - +1.1 3,968,514 + +0.9 3,968,549 + +1.3 3,968,564 + +1.7 3,968,564 + +1.9 3,968,626 + +2.6 3,968,646 + +0.3 3,968,646 + +0.8 3,968,646 + +3.7 3,968,646 + -0.1 3,968,646 + +0.3 3,968,646 + -1.8 3,968,646 + +1.5 3,968,646 + +0.3 3,968,646 + +3.1 3,968,646 + +1.1 3,968,646 + +0.1 3,968,646 + +1.0 3,968,646 + +1.1 3,968,646 + +0.8 3,968,654 - -0.5 3,968,654 - +0.1 3,968,654 - +2.1 3,968,654 - -1.0 3,968,654 - -0.7 3,968,654 - -1.3 3,968,654 - -0.9 3,968,654 - +2.1 3,968,654 - -2.0 3,968,654 - +0.1 3,968,654 - +1.1 3,968,654 - -0.3 3,968,656 - -2.5 3,968,666 + -0.6 3,968,666 + -0.2 3,968,674 - +0.4 3,968,674 - +1.0 3,968,698 + -0.0 3,968,706 - +0.8 3,968,719 + -0.3 3,968,755 + +1.0 3,968,820 + -0.2 3,968,831 + -0.3 3,968,863 + +0.8 3,968,867 + CCNA_03803 0.10 +1.1 3,968,875 - CCNA_03803 0.11 -1.3 3,968,903 - CCNA_03803 0.13 +0.7 3,968,913 + CCNA_03803 0.14 +0.6 3,968,913 + CCNA_03803 0.14 -1.2 3,968,913 + CCNA_03803 0.14 +1.6 3,968,986 - CCNA_03803 0.21 +2.1 3,968,986 - CCNA_03803 0.21 -0.3 3,968,988 + CCNA_03803 0.21 +0.1 3,968,990 - CCNA_03803 0.21 +1.8 3,968,992 - CCNA_03803 0.22 +0.5 3,969,004 + CCNA_03803 0.23 +0.3 3,969,020 + CCNA_03803 0.24 -0.9 3,969,024 + CCNA_03803 0.24 +0.1 3,969,025 + CCNA_03803 0.25 +0.6 3,969,025 + CCNA_03803 0.25 -1.1 3,969,033 - CCNA_03803 0.25 +0.2 3,969,088 + CCNA_03803 0.30 -0.3 3,969,142 - CCNA_03803 0.35 +0.1 3,969,167 - CCNA_03803 0.38 -0.5 3,969,207 + CCNA_03803 0.41 +0.7 3,969,207 + CCNA_03803 0.41 -0.5 3,969,207 + CCNA_03803 0.41 -0.3 3,969,207 + CCNA_03803 0.41 -0.2 3,969,215 - CCNA_03803 0.42 -1.5 3,969,263 - CCNA_03803 0.47 -0.3 3,969,277 + CCNA_03803 0.48 -0.5 3,969,378 + CCNA_03803 0.57 +0.5 3,969,378 + CCNA_03803 0.57 +1.5 3,969,378 + +1.1 3,969,378 + CCNA_03803 0.57 +1.1 3,969,378 + CCNA_03803 0.57 -0.5 3,969,378 + CCNA_03803 0.57 +1.3 3,969,386 - CCNA_03803 0.58 -1.8 3,969,386 - CCNA_03803 0.58 +2.1 3,969,386 - CCNA_03803 0.58 +2.1 3,969,402 + CCNA_03803 0.59 +0.5 3,969,450 + CCNA_03803 0.64 +0.5 3,969,450 + CCNA_03803 0.64 +1.4 3,969,450 + CCNA_03803 0.64 -0.3 3,969,458 - CCNA_03803 0.64 -1.1 3,969,458 - CCNA_03803 0.64 +0.5 3,969,458 - CCNA_03803 0.64 -0.7 3,969,491 + CCNA_03803 0.67 +0.4 3,969,517 + CCNA_03803 0.70 +0.9 3,969,517 + CCNA_03803 0.70 +1.0 3,969,517 + CCNA_03803 0.70 +0.6 3,969,517 + CCNA_03803 0.70 +0.6 3,969,525 - CCNA_03803 0.71 +0.5 3,969,558 - CCNA_03803 0.74 -0.0 3,969,579 + CCNA_03803 0.76 +0.5 3,969,579 + CCNA_03803 0.76 +0.7 3,969,579 + CCNA_03803 0.76 +0.4 3,969,597 - CCNA_03803 0.77 -0.4 3,969,607 - CCNA_03803 0.78 -0.1 3,969,613 + CCNA_03803 0.79 +0.9 3,969,666 + CCNA_03803 0.84 -0.3 3,969,673 + CCNA_03803 0.84 +1.3 3,969,688 + CCNA_03803 0.86 +0.7 3,969,688 + CCNA_03803 0.86 +1.0 3,969,747 - +1.4 3,969,747 - +1.1 3,969,772 - +1.5 3,969,774 + +0.3 3,969,791 - -1.6 3,969,793 + +4.1 3,969,803 - +0.4 3,969,837 + +0.7 3,969,837 + -1.4 3,969,845 - +2.3 3,969,845 - +2.9 3,969,906 + +0.8 3,970,009 + CCNA_03804 0.16 +1.1 3,970,009 + CCNA_03804 0.16 +0.7 3,970,032 + CCNA_03804 0.23 -0.9 3,970,042 + CCNA_03804 0.26 -0.7 3,970,085 - +2.7 3,970,085 - CCNA_03804 0.39 +0.1 3,970,085 - CCNA_03804 0.39 -0.9 3,970,085 - CCNA_03804 0.39 -1.0 3,970,085 - CCNA_03804 0.39 +2.3 3,970,121 - CCNA_03804 0.50 +0.4 3,970,166 - CCNA_03804 0.63 -1.1 3,970,167 + CCNA_03804 0.64 -0.0 3,970,172 + CCNA_03804 0.65 +0.5 3,970,172 + CCNA_03804 0.65 +1.5 3,970,172 + CCNA_03804 0.65 +1.1 3,970,180 - CCNA_03804 0.68 +1.5 3,970,180 - CCNA_03804 0.68 -0.9 3,970,180 - CCNA_03804 0.68 -0.5 3,970,191 + CCNA_03804 0.71 +1.1 3,970,208 + CCNA_03804 0.76 +0.3 3,970,254 + +0.5 3,970,254 + +0.7 3,970,254 + +0.4 3,970,294 - +2.4 3,970,321 - CCNA_03805 0.11 +3.1 3,970,328 - CCNA_03805 0.12 +3.8 3,970,328 - CCNA_03805 0.12 +3.0 3,970,367 - CCNA_03805 0.19 +3.2 3,970,367 - CCNA_03805 0.19 +3.0 3,970,369 - CCNA_03805 0.20 +3.1 3,970,388 - CCNA_03805 0.23 +3.2 3,970,417 - CCNA_03805 0.28 +3.3 3,970,417 - CCNA_03805 0.28 +3.0 3,970,417 - CCNA_03805 0.28 +2.7 3,970,439 - CCNA_03805 0.32 +3.2 3,970,441 - CCNA_03805 0.32 +3.0 3,970,464 - CCNA_03805 0.36 +3.4 3,970,483 - CCNA_03805 0.39 +3.0 3,970,505 - CCNA_03805 0.43 +3.3 3,970,505 - CCNA_03805 0.43 +3.7 3,970,544 - CCNA_03805 0.50 +3.1 3,970,544 - CCNA_03805 0.50 +3.1 3,970,567 - CCNA_03805 0.54 +3.2 3,970,572 - CCNA_03805 0.55 +3.0 3,970,572 - CCNA_03805 0.55 +2.9 3,970,631 - CCNA_03805 0.65 +3.3 3,970,690 - CCNA_03805 0.75 +2.8 3,970,690 - CCNA_03805 0.75 +3.1 3,970,737 + CCNA_03805 0.83 -0.7 3,970,745 - CCNA_03805 0.84 +1.2
Or see this region's nucleotide sequence