Experiment: PYE with benzoic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01321 and CCNA_01322 are separated by 3 nucleotides CCNA_01322 and CCNA_01323 are separated by 10 nucleotides CCNA_01323 and CCNA_01324 are separated by 3 nucleotides CCNA_01324 and CCNA_01325 are separated by 172 nucleotides CCNA_01325 and CCNA_01326 are separated by 219 nucleotides
CCNA_01321: CCNA_01321 - LSU ribosomal protein L6P, at 1,438,523 to 1,439,056
_01321
CCNA_01322: CCNA_01322 - LSU ribosomal protein L18P, at 1,439,060 to 1,439,410
_01322
CCNA_01323: CCNA_01323 - SSU ribosomal protein S5P, at 1,439,421 to 1,440,026
_01323
CCNA_01324: CCNA_01324 - LSU ribosomal protein L30P, at 1,440,030 to 1,440,215
_01324
CCNA_01325: CCNA_01325 - LSU ribosomal protein L15P, at 1,440,388 to 1,440,873
_01325
CCNA_01326: CCNA_01326 - protein translocase subunit secY, at 1,441,093 to 1,442,448
_01326
Position (kb) 1440
1441 Strain fitness (log2 ratio) -1
0
1
2
3 at 1440.117 kb on + strand, within CCNA_01324 at 1440.117 kb on + strand, within CCNA_01324 at 1440.137 kb on + strand, within CCNA_01324 at 1440.186 kb on + strand, within CCNA_01324 at 1440.186 kb on + strand, within CCNA_01324 at 1440.868 kb on + strand at 1440.875 kb on + strand at 1440.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with benzoic 0.2 mg/ml remove 1,440,117 + CCNA_01324 0.47 +2.7 1,440,117 + CCNA_01324 0.47 +3.1 1,440,137 + CCNA_01324 0.58 +2.5 1,440,186 + CCNA_01324 0.84 +3.3 1,440,186 + CCNA_01324 0.84 +3.1 1,440,868 + +3.7 1,440,875 + +3.3 1,440,992 + +2.4
Or see this region's nucleotide sequence