Experiment: PYE with benzoic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00147 and CCNA_00148 are separated by 75 nucleotides CCNA_00148 and CCNA_00149 are separated by 16 nucleotides CCNA_00149 and CCNA_00150 overlap by 4 nucleotides CCNA_00150 and CCNA_00151 are separated by 166 nucleotides
CCNA_00147: CCNA_00147 - hypothetical protein, at 158,354 to 158,470
_00147
CCNA_00148: CCNA_00148 - hypothetical protein, at 158,546 to 159,079
_00148
CCNA_00149: CCNA_00149 - Cro/CI-family transcriptional regulator, at 159,096 to 159,323
_00149
CCNA_00150: CCNA_00150 - PDZ domain family protein, at 159,320 to 160,297
_00150
CCNA_00151: CCNA_00151 - ribonuclease PH, at 160,464 to 161,180
_00151
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 158.334 kb on + strand at 158.366 kb on + strand, within CCNA_00147 at 158.434 kb on + strand, within CCNA_00147 at 158.463 kb on + strand at 158.463 kb on + strand at 158.464 kb on - strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.471 kb on - strand at 158.471 kb on - strand at 158.492 kb on - strand at 158.492 kb on - strand at 158.517 kb on + strand at 158.532 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand at 158.602 kb on + strand, within CCNA_00148 at 158.620 kb on - strand, within CCNA_00148 at 158.652 kb on - strand, within CCNA_00148 at 158.668 kb on - strand, within CCNA_00148 at 158.670 kb on + strand, within CCNA_00148 at 158.670 kb on - strand, within CCNA_00148 at 158.673 kb on - strand, within CCNA_00148 at 158.707 kb on + strand, within CCNA_00148 at 158.738 kb on + strand, within CCNA_00148 at 158.764 kb on + strand, within CCNA_00148 at 158.772 kb on - strand, within CCNA_00148 at 158.774 kb on + strand, within CCNA_00148 at 158.813 kb on + strand, within CCNA_00148 at 158.819 kb on + strand, within CCNA_00148 at 158.889 kb on - strand, within CCNA_00148 at 158.901 kb on + strand, within CCNA_00148 at 158.956 kb on + strand, within CCNA_00148 at 158.957 kb on + strand, within CCNA_00148 at 158.964 kb on - strand, within CCNA_00148 at 158.982 kb on - strand, within CCNA_00148 at 159.076 kb on - strand at 159.078 kb on + strand at 159.078 kb on + strand at 159.111 kb on + strand at 159.119 kb on - strand, within CCNA_00149 at 159.124 kb on + strand, within CCNA_00149 at 159.132 kb on - strand, within CCNA_00149 at 159.166 kb on - strand, within CCNA_00149 at 159.188 kb on + strand, within CCNA_00149 at 159.196 kb on - strand, within CCNA_00149 at 159.203 kb on - strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.227 kb on + strand, within CCNA_00149 at 159.230 kb on - strand, within CCNA_00149 at 159.266 kb on - strand, within CCNA_00149 at 159.279 kb on + strand, within CCNA_00149 at 159.345 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.354 kb on - strand at 159.354 kb on - strand at 159.370 kb on + strand at 159.391 kb on - strand at 159.402 kb on + strand at 159.520 kb on - strand, within CCNA_00150 at 159.531 kb on + strand, within CCNA_00150 at 159.536 kb on + strand, within CCNA_00150 at 159.613 kb on + strand, within CCNA_00150 at 159.613 kb on + strand, within CCNA_00150 at 159.619 kb on + strand, within CCNA_00150 at 159.621 kb on - strand, within CCNA_00150 at 159.651 kb on + strand, within CCNA_00150 at 159.683 kb on - strand, within CCNA_00150 at 159.721 kb on - strand, within CCNA_00150 at 159.756 kb on + strand, within CCNA_00150 at 159.758 kb on - strand, within CCNA_00150 at 159.761 kb on + strand, within CCNA_00150 at 159.769 kb on - strand, within CCNA_00150 at 159.769 kb on - strand, within CCNA_00150 at 159.769 kb on - strand, within CCNA_00150 at 159.769 kb on - strand, within CCNA_00150 at 159.776 kb on + strand, within CCNA_00150 at 159.809 kb on - strand, within CCNA_00150 at 159.821 kb on + strand, within CCNA_00150 at 159.874 kb on - strand, within CCNA_00150 at 159.978 kb on + strand, within CCNA_00150 at 159.990 kb on - strand, within CCNA_00150 at 159.995 kb on + strand, within CCNA_00150 at 160.013 kb on + strand, within CCNA_00150 at 160.018 kb on + strand, within CCNA_00150 at 160.026 kb on - strand, within CCNA_00150 at 160.026 kb on - strand, within CCNA_00150 at 160.056 kb on + strand, within CCNA_00150 at 160.116 kb on - strand, within CCNA_00150 at 160.121 kb on - strand, within CCNA_00150 at 160.154 kb on - strand at 160.172 kb on - strand, within CCNA_00150 at 160.172 kb on - strand, within CCNA_00150 at 160.176 kb on + strand, within CCNA_00150 at 160.190 kb on - strand, within CCNA_00150 at 160.203 kb on + strand at 160.211 kb on - strand at 160.215 kb on + strand at 160.220 kb on + strand at 160.238 kb on + strand at 160.241 kb on - strand at 160.246 kb on - strand at 160.259 kb on - strand at 160.300 kb on - strand at 160.304 kb on + strand at 160.304 kb on + strand at 160.304 kb on + strand at 160.304 kb on + strand at 160.305 kb on - strand at 160.312 kb on - strand at 160.312 kb on - strand at 160.314 kb on - strand at 160.314 kb on - strand at 160.329 kb on + strand at 160.337 kb on - strand at 160.396 kb on - strand at 160.405 kb on + strand at 160.406 kb on - strand at 160.406 kb on - strand at 160.413 kb on + strand at 160.426 kb on + strand at 160.455 kb on + strand at 160.455 kb on + strand at 160.455 kb on + strand at 160.456 kb on + strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.480 kb on + strand at 160.511 kb on - strand at 160.520 kb on - strand at 160.571 kb on - strand, within CCNA_00151 at 160.571 kb on - strand, within CCNA_00151 at 160.605 kb on + strand, within CCNA_00151 at 160.641 kb on - strand, within CCNA_00151 at 160.646 kb on - strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.699 kb on + strand, within CCNA_00151 at 160.726 kb on + strand, within CCNA_00151 at 160.734 kb on - strand, within CCNA_00151 at 160.755 kb on - strand, within CCNA_00151 at 160.761 kb on + strand at 160.774 kb on - strand, within CCNA_00151 at 160.780 kb on - strand, within CCNA_00151 at 160.796 kb on - strand, within CCNA_00151 at 160.848 kb on - strand, within CCNA_00151 at 160.864 kb on + strand, within CCNA_00151 at 160.873 kb on - strand, within CCNA_00151 at 160.940 kb on - strand, within CCNA_00151 at 160.989 kb on + strand, within CCNA_00151 at 160.999 kb on + strand, within CCNA_00151 at 160.999 kb on + strand, within CCNA_00151 at 161.002 kb on - strand, within CCNA_00151 at 161.007 kb on - strand, within CCNA_00151 at 161.025 kb on - strand, within CCNA_00151 at 161.046 kb on + strand, within CCNA_00151 at 161.054 kb on - strand, within CCNA_00151 at 161.054 kb on - strand, within CCNA_00151 at 161.067 kb on - strand, within CCNA_00151 at 161.072 kb on - strand, within CCNA_00151 at 161.079 kb on - strand, within CCNA_00151 at 161.089 kb on - strand, within CCNA_00151 at 161.104 kb on - strand, within CCNA_00151 at 161.106 kb on - strand, within CCNA_00151 at 161.135 kb on - strand at 161.135 kb on - strand at 161.143 kb on + strand at 161.143 kb on + strand at 161.151 kb on - strand at 161.151 kb on - strand at 161.161 kb on - strand at 161.187 kb on + strand at 161.187 kb on + strand at 161.195 kb on - strand at 161.205 kb on + strand at 161.221 kb on - strand at 161.221 kb on - strand at 161.221 kb on - strand at 161.244 kb on - strand at 161.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with benzoic 0.2 mg/ml remove 158,334 + +0.6 158,366 + CCNA_00147 0.10 +1.1 158,434 + CCNA_00147 0.68 +1.7 158,463 + +1.7 158,463 + +1.7 158,464 - -1.0 158,465 + +1.9 158,465 + +1.4 158,465 + +1.7 158,465 + +2.1 158,471 - -1.7 158,471 - +0.1 158,492 - +0.1 158,492 - +0.1 158,517 + +1.8 158,532 + +2.1 158,553 + +0.9 158,553 + +1.6 158,553 + +1.3 158,602 + CCNA_00148 0.10 +2.1 158,620 - CCNA_00148 0.14 -2.0 158,652 - CCNA_00148 0.20 -1.5 158,668 - CCNA_00148 0.23 +0.3 158,670 + CCNA_00148 0.23 +1.3 158,670 - CCNA_00148 0.23 +1.2 158,673 - CCNA_00148 0.24 +0.1 158,707 + CCNA_00148 0.30 +0.8 158,738 + CCNA_00148 0.36 +0.5 158,764 + CCNA_00148 0.41 +1.3 158,772 - CCNA_00148 0.42 -0.2 158,774 + CCNA_00148 0.43 +1.6 158,813 + CCNA_00148 0.50 +0.1 158,819 + CCNA_00148 0.51 +0.7 158,889 - CCNA_00148 0.64 -0.7 158,901 + CCNA_00148 0.66 +1.0 158,956 + CCNA_00148 0.77 -0.5 158,957 + CCNA_00148 0.77 +1.3 158,964 - CCNA_00148 0.78 -1.2 158,982 - CCNA_00148 0.82 -1.8 159,076 - -1.6 159,078 + +2.6 159,078 + +2.3 159,111 + +4.8 159,119 - CCNA_00149 0.10 -0.2 159,124 + CCNA_00149 0.12 +1.6 159,132 - CCNA_00149 0.16 -0.3 159,166 - CCNA_00149 0.31 +0.2 159,188 + CCNA_00149 0.40 +2.4 159,196 - CCNA_00149 0.44 +1.5 159,203 - CCNA_00149 0.47 +3.1 159,227 + CCNA_00149 0.57 +2.4 159,227 + CCNA_00149 0.57 +2.3 159,227 + CCNA_00149 0.57 +2.2 159,230 - CCNA_00149 0.59 -1.1 159,266 - CCNA_00149 0.75 -0.5 159,279 + CCNA_00149 0.80 +2.8 159,345 + +0.5 159,346 + +2.0 159,346 + +1.6 159,354 - +2.0 159,354 - +2.0 159,370 + +1.1 159,391 - +1.0 159,402 + +1.4 159,520 - CCNA_00150 0.20 +1.5 159,531 + CCNA_00150 0.22 +0.3 159,536 + CCNA_00150 0.22 +1.5 159,613 + CCNA_00150 0.30 -0.2 159,613 + CCNA_00150 0.30 +1.5 159,619 + CCNA_00150 0.31 +1.6 159,621 - CCNA_00150 0.31 +1.5 159,651 + CCNA_00150 0.34 -0.0 159,683 - CCNA_00150 0.37 +2.4 159,721 - CCNA_00150 0.41 +2.1 159,756 + CCNA_00150 0.45 +0.8 159,758 - CCNA_00150 0.45 +2.0 159,761 + CCNA_00150 0.45 +1.1 159,769 - CCNA_00150 0.46 +2.0 159,769 - CCNA_00150 0.46 +2.3 159,769 - CCNA_00150 0.46 +1.7 159,769 - CCNA_00150 0.46 +2.8 159,776 + CCNA_00150 0.47 -1.3 159,809 - CCNA_00150 0.50 +2.1 159,821 + CCNA_00150 0.51 +1.9 159,874 - CCNA_00150 0.57 +0.8 159,978 + CCNA_00150 0.67 -0.7 159,990 - CCNA_00150 0.69 +2.1 159,995 + CCNA_00150 0.69 +1.2 160,013 + CCNA_00150 0.71 +0.5 160,018 + CCNA_00150 0.71 +0.3 160,026 - CCNA_00150 0.72 +1.7 160,026 - CCNA_00150 0.72 +2.2 160,056 + CCNA_00150 0.75 -0.9 160,116 - CCNA_00150 0.81 +1.7 160,121 - CCNA_00150 0.82 +2.4 160,154 - +3.7 160,172 - CCNA_00150 0.87 +2.9 160,172 - CCNA_00150 0.87 +1.1 160,176 + CCNA_00150 0.88 -0.1 160,190 - CCNA_00150 0.89 +2.0 160,203 + -0.0 160,211 - +2.4 160,215 + -3.2 160,220 + -1.6 160,238 + +0.9 160,241 - +2.8 160,246 - +2.4 160,259 - +2.0 160,300 - +1.6 160,304 + -0.2 160,304 + +0.1 160,304 + -0.3 160,304 + +0.8 160,305 - +2.0 160,312 - +2.1 160,312 - +1.8 160,314 - +1.9 160,314 - +2.2 160,329 + -1.0 160,337 - +2.1 160,396 - +1.7 160,405 + -0.5 160,406 - +2.2 160,406 - +1.9 160,413 + +3.1 160,426 + +0.9 160,455 + -0.7 160,455 + +0.4 160,455 + -0.9 160,456 + +0.0 160,463 - +1.4 160,463 - +0.9 160,463 - +2.1 160,463 - +2.0 160,463 - +1.4 160,480 + -1.9 160,511 - +1.1 160,520 - +1.4 160,571 - CCNA_00151 0.15 +1.2 160,571 - CCNA_00151 0.15 +0.5 160,605 + CCNA_00151 0.20 -0.9 160,641 - CCNA_00151 0.25 +0.9 160,646 - CCNA_00151 0.25 +0.1 160,675 + CCNA_00151 0.29 +1.1 160,675 + CCNA_00151 0.29 -1.3 160,675 + CCNA_00151 0.29 -0.2 160,683 - CCNA_00151 0.31 +1.6 160,683 - CCNA_00151 0.31 +0.5 160,683 - CCNA_00151 0.31 +1.1 160,683 - CCNA_00151 0.31 +0.6 160,683 - CCNA_00151 0.31 +2.6 160,683 - CCNA_00151 0.31 -0.6 160,683 - CCNA_00151 0.31 +0.3 160,699 + CCNA_00151 0.33 -1.0 160,726 + CCNA_00151 0.37 -1.9 160,734 - CCNA_00151 0.38 +0.7 160,755 - CCNA_00151 0.41 +0.1 160,761 + +0.5 160,774 - CCNA_00151 0.43 +0.3 160,780 - CCNA_00151 0.44 +2.1 160,796 - CCNA_00151 0.46 +0.9 160,848 - CCNA_00151 0.54 +0.7 160,864 + CCNA_00151 0.56 -1.3 160,873 - CCNA_00151 0.57 +0.8 160,940 - CCNA_00151 0.66 +0.1 160,989 + CCNA_00151 0.73 +0.2 160,999 + CCNA_00151 0.75 +2.1 160,999 + CCNA_00151 0.75 -2.3 161,002 - CCNA_00151 0.75 +0.0 161,007 - CCNA_00151 0.76 -0.5 161,025 - CCNA_00151 0.78 +1.1 161,046 + CCNA_00151 0.81 -0.1 161,054 - CCNA_00151 0.82 +1.0 161,054 - CCNA_00151 0.82 +0.3 161,067 - CCNA_00151 0.84 +0.0 161,072 - CCNA_00151 0.85 -0.2 161,079 - CCNA_00151 0.86 -0.4 161,089 - CCNA_00151 0.87 -0.2 161,104 - CCNA_00151 0.89 -1.1 161,106 - CCNA_00151 0.90 -1.3 161,135 - +1.3 161,135 - -0.2 161,143 + -0.0 161,143 + +1.1 161,151 - +2.1 161,151 - +1.8 161,161 - +0.3 161,187 + +2.1 161,187 + -1.0 161,195 - +1.1 161,205 + +0.5 161,221 - -0.2 161,221 - -1.0 161,221 - +0.7 161,244 - +0.5 161,277 - +0.1
Or see this region's nucleotide sequence