Experiment: Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0024 and Shew_0025 are separated by 272 nucleotides Shew_0025 and Shew_0026 are separated by 115 nucleotides Shew_0026 and Shew_0027 are separated by 12 nucleotides Shew_0027 and Shew_0028 are separated by 81 nucleotides
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Shew_0025: Shew_0025 - protoporphyrinogen oxidase (RefSeq), at 28,183 to 28,716
_0025
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Shew_0027: Shew_0027 - glutathione S-transferase domain-containing protein (RefSeq), at 29,381 to 30,028
_0027
Shew_0028: Shew_0028 - LysR family transcriptional regulator (RefSeq), at 30,110 to 31,009
_0028
Position (kb) 28
29
30 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 27.851 kb on - strand, within Shew_0024 at 28.301 kb on + strand, within Shew_0025 at 28.365 kb on - strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.442 kb on + strand, within Shew_0025 at 28.700 kb on - strand at 28.750 kb on + strand at 29.027 kb on - strand, within Shew_0026 at 29.135 kb on + strand, within Shew_0026 at 29.328 kb on - strand at 29.371 kb on - strand at 29.774 kb on - strand, within Shew_0027 at 29.889 kb on + strand, within Shew_0027 at 30.115 kb on - strand at 30.128 kb on - strand at 30.324 kb on - strand, within Shew_0028
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 10 mM remove 27,851 - Shew_0024 0.80 -3.8 28,301 + Shew_0025 0.22 +0.5 28,365 - Shew_0025 0.34 +0.1 28,401 + Shew_0025 0.41 +0.1 28,401 + Shew_0025 0.41 +0.8 28,401 + Shew_0025 0.41 -0.7 28,442 + Shew_0025 0.49 +0.5 28,700 - -0.1 28,750 + -1.0 29,027 - Shew_0026 0.36 -0.5 29,135 + Shew_0026 0.56 -0.0 29,328 - -0.4 29,371 - +0.1 29,774 - Shew_0027 0.61 +0.4 29,889 + Shew_0027 0.78 -0.3 30,115 - +0.1 30,128 - -0.5 30,324 - Shew_0028 0.24 -0.1
Or see this region's nucleotide sequence