Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS15760 and PFLU_RS15765 overlap by 4 nucleotides PFLU_RS15765 and PFLU_RS15770 are separated by 47 nucleotides PFLU_RS15770 and PFLU_RS15775 are separated by 146 nucleotides PFLU_RS15775 and PFLU_RS15780 are separated by 6 nucleotides
PFLU_RS15760: PFLU_RS15760 - general secretion pathway protein GspL, at 3,576,055 to 3,577,227
_RS15760
PFLU_RS15765: PFLU_RS15765 - general secretion pathway protein GspK, at 3,577,224 to 3,578,150
_RS15765
PFLU_RS15770: PFLU_RS15770 - type II secretion system protein GspG, at 3,578,198 to 3,578,647
_RS15770
PFLU_RS15775: PFLU_RS15775 - type II secretion system protein GspI, at 3,578,794 to 3,579,180
_RS15775
PFLU_RS15780: PFLU_RS15780 - general secretion pathway protein GspC, at 3,579,187 to 3,579,618
_RS15780
Position (kb)
3578
3579 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3577.227 kb on + strand at 3577.228 kb on - strand at 3577.276 kb on + strand at 3577.277 kb on - strand at 3577.277 kb on - strand at 3577.277 kb on - strand at 3577.422 kb on - strand, within PFLU_RS15765 at 3577.500 kb on + strand, within PFLU_RS15765 at 3577.501 kb on - strand, within PFLU_RS15765 at 3577.536 kb on - strand, within PFLU_RS15765 at 3577.558 kb on + strand, within PFLU_RS15765 at 3577.558 kb on + strand, within PFLU_RS15765 at 3577.558 kb on + strand, within PFLU_RS15765 at 3577.558 kb on + strand, within PFLU_RS15765 at 3577.559 kb on - strand, within PFLU_RS15765 at 3577.818 kb on + strand, within PFLU_RS15765 at 3578.310 kb on + strand, within PFLU_RS15770 at 3578.310 kb on + strand, within PFLU_RS15770 at 3578.311 kb on - strand, within PFLU_RS15770 at 3578.425 kb on - strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.527 kb on + strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.528 kb on - strand, within PFLU_RS15770 at 3578.531 kb on + strand, within PFLU_RS15770 at 3578.532 kb on - strand, within PFLU_RS15770 at 3578.532 kb on - strand, within PFLU_RS15770 at 3578.532 kb on - strand, within PFLU_RS15770 at 3578.688 kb on + strand at 3578.688 kb on + strand at 3578.689 kb on - strand at 3578.689 kb on - strand at 3578.701 kb on - strand at 3578.701 kb on - strand at 3578.709 kb on + strand at 3578.709 kb on + strand at 3578.760 kb on - strand at 3578.823 kb on + strand at 3578.823 kb on + strand at 3579.611 kb on + strand at 3579.611 kb on + strand at 3579.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 3,577,227 + -1.1 3,577,228 - +0.7 3,577,276 + +2.5 3,577,277 - +1.2 3,577,277 - +1.5 3,577,277 - -1.1 3,577,422 - PFLU_RS15765 0.21 +0.9 3,577,500 + PFLU_RS15765 0.30 -1.2 3,577,501 - PFLU_RS15765 0.30 +1.2 3,577,536 - PFLU_RS15765 0.34 -0.4 3,577,558 + PFLU_RS15765 0.36 -0.5 3,577,558 + PFLU_RS15765 0.36 -1.4 3,577,558 + PFLU_RS15765 0.36 -0.3 3,577,558 + PFLU_RS15765 0.36 +0.6 3,577,559 - PFLU_RS15765 0.36 -1.4 3,577,818 + PFLU_RS15765 0.64 -2.9 3,578,310 + PFLU_RS15770 0.25 -2.5 3,578,310 + PFLU_RS15770 0.25 -2.8 3,578,311 - PFLU_RS15770 0.25 -1.4 3,578,425 - PFLU_RS15770 0.50 +0.5 3,578,527 + PFLU_RS15770 0.73 +0.5 3,578,527 + PFLU_RS15770 0.73 +0.9 3,578,527 + PFLU_RS15770 0.73 -0.2 3,578,527 + PFLU_RS15770 0.73 -1.0 3,578,527 + PFLU_RS15770 0.73 +1.9 3,578,527 + PFLU_RS15770 0.73 -0.9 3,578,527 + PFLU_RS15770 0.73 -0.1 3,578,528 - PFLU_RS15770 0.73 -1.6 3,578,528 - PFLU_RS15770 0.73 +0.3 3,578,528 - PFLU_RS15770 0.73 -0.8 3,578,528 - PFLU_RS15770 0.73 +1.7 3,578,528 - PFLU_RS15770 0.73 +1.4 3,578,528 - PFLU_RS15770 0.73 +0.3 3,578,531 + PFLU_RS15770 0.74 -2.1 3,578,532 - PFLU_RS15770 0.74 -0.3 3,578,532 - PFLU_RS15770 0.74 +0.1 3,578,532 - PFLU_RS15770 0.74 +1.7 3,578,688 + -0.1 3,578,688 + +0.9 3,578,689 - +0.3 3,578,689 - -0.1 3,578,701 - +1.5 3,578,701 - -1.0 3,578,709 + +0.2 3,578,709 + -0.1 3,578,760 - -1.4 3,578,823 + -0.4 3,578,823 + +0.0 3,579,611 + +0.1 3,579,611 + +0.9 3,579,612 - +1.9
Or see this region's nucleotide sequence