Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folE and pyrE are separated by 81 nucleotides pyrE and Shew_3488 are separated by 83 nucleotides Shew_3488 and Shew_3489 are separated by 181 nucleotides
Shew_3486: folE - GTP cyclohydrolase I (RefSeq), at 4,172,245 to 4,172,889
folE
Shew_3487: pyrE - orotate phosphoribosyltransferase (RefSeq), at 4,172,971 to 4,173,612
pyrE
Shew_3488: Shew_3488 - ribonuclease PH (RefSeq), at 4,173,696 to 4,174,409
_3488
Shew_3489: Shew_3489 - hypothetical protein (RefSeq), at 4,174,591 to 4,175,454
_3489
Position (kb)
4173
4174
4175 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4173.284 kb on - strand, within pyrE at 4173.488 kb on + strand, within pyrE at 4173.488 kb on + strand, within pyrE at 4173.496 kb on - strand, within pyrE at 4173.526 kb on - strand, within pyrE at 4173.556 kb on + strand at 4173.612 kb on - strand at 4173.750 kb on - strand at 4173.897 kb on + strand, within Shew_3488 at 4173.963 kb on - strand, within Shew_3488 at 4173.996 kb on + strand, within Shew_3488 at 4174.001 kb on + strand, within Shew_3488 at 4174.009 kb on - strand, within Shew_3488 at 4174.066 kb on - strand, within Shew_3488 at 4174.306 kb on - strand, within Shew_3488 at 4174.353 kb on - strand at 4174.356 kb on - strand at 4174.358 kb on + strand at 4174.390 kb on - strand at 4174.472 kb on + strand at 4174.530 kb on - strand at 4174.599 kb on + strand at 4174.617 kb on - strand at 4174.641 kb on - strand at 4174.721 kb on + strand, within Shew_3489 at 4174.729 kb on - strand, within Shew_3489 at 4174.729 kb on - strand, within Shew_3489 at 4175.194 kb on + strand, within Shew_3489 at 4175.202 kb on - strand, within Shew_3489 at 4175.228 kb on - strand, within Shew_3489 at 4175.346 kb on + strand, within Shew_3489 at 4175.375 kb on + strand at 4175.383 kb on - strand at 4175.383 kb on - strand at 4175.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 4,173,284 - pyrE Shew_3487 0.49 +1.8 4,173,488 + pyrE Shew_3487 0.81 +2.8 4,173,488 + pyrE Shew_3487 0.81 +1.5 4,173,496 - pyrE Shew_3487 0.82 +1.7 4,173,526 - pyrE Shew_3487 0.86 +2.1 4,173,556 + +1.3 4,173,612 - +1.8 4,173,750 - +1.0 4,173,897 + Shew_3488 0.28 +3.3 4,173,963 - Shew_3488 0.37 +3.1 4,173,996 + Shew_3488 0.42 +3.5 4,174,001 + Shew_3488 0.43 +4.2 4,174,009 - Shew_3488 0.44 +1.9 4,174,066 - Shew_3488 0.52 +1.6 4,174,306 - Shew_3488 0.85 +1.8 4,174,353 - +1.3 4,174,356 - +1.3 4,174,358 + +3.6 4,174,390 - +2.9 4,174,472 + +2.0 4,174,530 - +0.1 4,174,599 + +0.3 4,174,617 - +0.6 4,174,641 - -0.7 4,174,721 + Shew_3489 0.15 +0.8 4,174,729 - Shew_3489 0.16 +0.0 4,174,729 - Shew_3489 0.16 +0.2 4,175,194 + Shew_3489 0.70 +0.2 4,175,202 - Shew_3489 0.71 +0.6 4,175,228 - Shew_3489 0.74 +0.8 4,175,346 + Shew_3489 0.87 +0.1 4,175,375 + +0.1 4,175,383 - +0.5 4,175,383 - +0.7 4,175,383 - +0.1
Or see this region's nucleotide sequence