Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3430 and aceE are separated by 11 nucleotides aceE and pdhR are separated by 69 nucleotides
Shew_3430: Shew_3430 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (RefSeq), at 4,093,917 to 4,095,869
_3430
Shew_3431: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 4,095,881 to 4,098,547
aceE
Shew_3432: pdhR - transcriptional regulator PdhR (RefSeq), at 4,098,617 to 4,099,369
pdhR
Position (kb)
4095
4096
4097
4098
4099 Strain fitness (log2 ratio)
-1
0
1
2 at 4095.353 kb on + strand, within Shew_3430 at 4095.487 kb on - strand, within Shew_3430 at 4096.068 kb on + strand at 4096.070 kb on + strand at 4096.365 kb on + strand, within aceE at 4096.365 kb on + strand, within aceE at 4096.371 kb on + strand, within aceE at 4096.373 kb on - strand, within aceE at 4096.557 kb on + strand, within aceE at 4096.575 kb on - strand, within aceE at 4096.625 kb on + strand, within aceE at 4096.656 kb on - strand, within aceE at 4096.866 kb on - strand, within aceE at 4096.885 kb on - strand, within aceE at 4096.957 kb on - strand, within aceE at 4097.161 kb on + strand, within aceE at 4097.209 kb on - strand, within aceE at 4097.389 kb on + strand, within aceE at 4097.389 kb on + strand, within aceE at 4097.444 kb on - strand, within aceE at 4097.549 kb on + strand, within aceE at 4097.562 kb on + strand, within aceE at 4097.892 kb on - strand, within aceE at 4097.997 kb on + strand, within aceE at 4098.011 kb on - strand, within aceE at 4098.643 kb on + strand at 4098.646 kb on - strand at 4098.953 kb on + strand, within pdhR at 4099.029 kb on + strand, within pdhR at 4099.185 kb on - strand, within pdhR at 4099.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 4,095,353 + Shew_3430 0.74 +1.9 4,095,487 - Shew_3430 0.80 +1.0 4,096,068 + +1.7 4,096,070 + +1.4 4,096,365 + aceE Shew_3431 0.18 +1.8 4,096,365 + aceE Shew_3431 0.18 +2.2 4,096,371 + aceE Shew_3431 0.18 +1.8 4,096,373 - aceE Shew_3431 0.18 +1.7 4,096,557 + aceE Shew_3431 0.25 +1.0 4,096,575 - aceE Shew_3431 0.26 +2.0 4,096,625 + aceE Shew_3431 0.28 +2.1 4,096,656 - aceE Shew_3431 0.29 +1.4 4,096,866 - aceE Shew_3431 0.37 +1.9 4,096,885 - aceE Shew_3431 0.38 +2.0 4,096,957 - aceE Shew_3431 0.40 +1.9 4,097,161 + aceE Shew_3431 0.48 +1.6 4,097,209 - aceE Shew_3431 0.50 +2.7 4,097,389 + aceE Shew_3431 0.57 +2.2 4,097,389 + aceE Shew_3431 0.57 +1.8 4,097,444 - aceE Shew_3431 0.59 +1.7 4,097,549 + aceE Shew_3431 0.63 +1.8 4,097,562 + aceE Shew_3431 0.63 +1.8 4,097,892 - aceE Shew_3431 0.75 +1.9 4,097,997 + aceE Shew_3431 0.79 +2.0 4,098,011 - aceE Shew_3431 0.80 +2.1 4,098,643 + +2.3 4,098,646 - +1.1 4,098,953 + pdhR Shew_3432 0.45 +2.3 4,099,029 + pdhR Shew_3432 0.55 +2.0 4,099,185 - pdhR Shew_3432 0.75 +0.8 4,099,515 + +0.5
Or see this region's nucleotide sequence