Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3246 and Shew_R0107 are separated by 237 nucleotides Shew_R0107 and Shew_R0108 are separated by 51 nucleotides Shew_R0108 and Shew_3247 are separated by 312 nucleotides Shew_3247 and Shew_3248 are separated by 132 nucleotides Shew_3248 and Shew_3249 are separated by 229 nucleotides
Shew_3246: Shew_3246 - hypothetical protein (RefSeq), at 3,883,355 to 3,884,032
_3246
Shew_R0107: Shew_R0107 - tRNA-Leu (RefSeq), at 3,884,270 to 3,884,356
_R0107
Shew_R0108: Shew_R0108 - tRNA-Leu (RefSeq), at 3,884,408 to 3,884,492
_R0108
Shew_3247: Shew_3247 - hypothetical protein (RefSeq), at 3,884,805 to 3,884,933
_3247
Shew_3248: Shew_3248 - hypothetical protein (RefSeq), at 3,885,066 to 3,885,677
_3248
Shew_3249: Shew_3249 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 3,885,907 to 3,887,547
_3249
Position (kb)
3884
3885 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3883.809 kb on + strand, within Shew_3246 at 3883.855 kb on + strand, within Shew_3246 at 3883.863 kb on - strand, within Shew_3246 at 3883.946 kb on - strand, within Shew_3246 at 3883.951 kb on - strand, within Shew_3246 at 3884.000 kb on - strand at 3884.038 kb on + strand at 3884.046 kb on - strand at 3884.046 kb on - strand at 3884.105 kb on - strand at 3884.542 kb on - strand at 3884.888 kb on + strand, within Shew_3247 at 3885.097 kb on - strand at 3885.176 kb on - strand, within Shew_3248 at 3885.329 kb on - strand, within Shew_3248 at 3885.432 kb on - strand, within Shew_3248 at 3885.432 kb on - strand, within Shew_3248 at 3885.547 kb on - strand, within Shew_3248 at 3885.587 kb on - strand, within Shew_3248 at 3885.602 kb on - strand, within Shew_3248 at 3885.824 kb on + strand at 3885.832 kb on - strand at 3885.838 kb on + strand at 3885.839 kb on - strand at 3885.846 kb on - strand at 3885.925 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 3,883,809 + Shew_3246 0.67 +0.4 3,883,855 + Shew_3246 0.74 -0.4 3,883,863 - Shew_3246 0.75 -2.6 3,883,946 - Shew_3246 0.87 +0.4 3,883,951 - Shew_3246 0.88 +0.3 3,884,000 - +0.2 3,884,038 + +0.0 3,884,046 - +2.7 3,884,046 - +0.1 3,884,105 - +0.2 3,884,542 - -1.3 3,884,888 + Shew_3247 0.64 -1.1 3,885,097 - +0.2 3,885,176 - Shew_3248 0.18 +1.0 3,885,329 - Shew_3248 0.43 +0.3 3,885,432 - Shew_3248 0.60 -0.7 3,885,432 - Shew_3248 0.60 -0.1 3,885,547 - Shew_3248 0.79 +0.5 3,885,587 - Shew_3248 0.85 +0.2 3,885,602 - Shew_3248 0.88 -0.0 3,885,824 + +0.2 3,885,832 - +0.3 3,885,838 + -0.2 3,885,839 - -0.8 3,885,846 - +0.2 3,885,925 - -0.4
Or see this region's nucleotide sequence