Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2884 and Shew_2885 are separated by 129 nucleotides Shew_2885 and Shew_2886 are separated by 110 nucleotides Shew_2886 and Shew_2887 are separated by 174 nucleotides
Shew_2884: Shew_2884 - glycine cleavage system regulatory protein-like protein (RefSeq), at 3,423,463 to 3,423,966
_2884
Shew_2885: Shew_2885 - 2OG-Fe(II) oxygenase (RefSeq), at 3,424,096 to 3,424,743
_2885
Shew_2886: Shew_2886 - rRNA (guanine-N(2)-)-methyltransferase (RefSeq), at 3,424,854 to 3,426,059
_2886
Shew_2887: Shew_2887 - sterol desaturase family protein (RefSeq), at 3,426,234 to 3,427,148
_2887
Position (kb)
3424
3425
3426
3427 Strain fitness (log2 ratio)
-1
0
1
2 at 3423.918 kb on + strand at 3424.063 kb on - strand at 3424.073 kb on - strand at 3424.073 kb on - strand at 3424.160 kb on - strand at 3424.247 kb on + strand, within Shew_2885 at 3424.247 kb on + strand, within Shew_2885 at 3424.258 kb on + strand, within Shew_2885 at 3424.271 kb on - strand, within Shew_2885 at 3424.317 kb on - strand, within Shew_2885 at 3424.457 kb on - strand, within Shew_2885 at 3424.457 kb on - strand, within Shew_2885 at 3424.493 kb on - strand, within Shew_2885 at 3424.519 kb on + strand, within Shew_2885 at 3424.650 kb on + strand, within Shew_2885 at 3424.796 kb on - strand at 3424.858 kb on - strand at 3424.997 kb on - strand, within Shew_2886 at 3425.193 kb on - strand, within Shew_2886 at 3425.193 kb on - strand, within Shew_2886 at 3425.329 kb on - strand, within Shew_2886 at 3425.452 kb on + strand, within Shew_2886 at 3425.533 kb on - strand, within Shew_2886 at 3425.808 kb on - strand, within Shew_2886 at 3425.810 kb on + strand, within Shew_2886 at 3426.094 kb on + strand at 3426.245 kb on + strand at 3426.253 kb on - strand at 3426.495 kb on + strand, within Shew_2887 at 3426.516 kb on - strand, within Shew_2887
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 3,423,918 + -0.1 3,424,063 - +0.2 3,424,073 - -0.0 3,424,073 - +0.1 3,424,160 - -0.1 3,424,247 + Shew_2885 0.23 -0.4 3,424,247 + Shew_2885 0.23 -0.0 3,424,258 + Shew_2885 0.25 +0.0 3,424,271 - Shew_2885 0.27 +0.2 3,424,317 - Shew_2885 0.34 -0.0 3,424,457 - Shew_2885 0.56 -0.4 3,424,457 - Shew_2885 0.56 -0.5 3,424,493 - Shew_2885 0.61 -0.2 3,424,519 + Shew_2885 0.65 +0.3 3,424,650 + Shew_2885 0.85 -0.2 3,424,796 - -0.1 3,424,858 - -1.0 3,424,997 - Shew_2886 0.12 -0.8 3,425,193 - Shew_2886 0.28 -0.7 3,425,193 - Shew_2886 0.28 -0.8 3,425,329 - Shew_2886 0.39 -0.9 3,425,452 + Shew_2886 0.50 -0.9 3,425,533 - Shew_2886 0.56 -0.7 3,425,808 - Shew_2886 0.79 -0.5 3,425,810 + Shew_2886 0.79 -0.7 3,426,094 + -0.1 3,426,245 + +2.7 3,426,253 - +0.2 3,426,495 + Shew_2887 0.29 +1.0 3,426,516 - Shew_2887 0.31 +0.4
Or see this region's nucleotide sequence