Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1593 and Shew_1594 are separated by 569 nucleotides Shew_1594 and Shew_1595 overlap by 4 nucleotides Shew_1595 and Shew_1596 overlap by 20 nucleotides
Shew_1593: Shew_1593 - ribonuclease (RefSeq), at 1,823,406 to 1,826,792
_1593
Shew_1594: Shew_1594 - RluA family pseudouridine synthase (RefSeq), at 1,827,362 to 1,828,351
_1594
Shew_1595: Shew_1595 - HAD family hydrolase (RefSeq), at 1,828,348 to 1,829,007
_1595
Shew_1596: Shew_1596 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 1,828,988 to 1,830,361
_1596
Position (kb)
1827
1828
1829 Strain fitness (log2 ratio)
-1
0
1 at 1827.352 kb on + strand at 1827.427 kb on + strand at 1827.442 kb on + strand at 1827.450 kb on - strand at 1827.713 kb on - strand, within Shew_1594 at 1828.044 kb on - strand, within Shew_1594 at 1828.102 kb on - strand, within Shew_1594 at 1828.306 kb on + strand at 1828.551 kb on + strand, within Shew_1595 at 1828.701 kb on - strand, within Shew_1595 at 1828.775 kb on - strand, within Shew_1595 at 1828.900 kb on - strand, within Shew_1595 at 1828.910 kb on - strand, within Shew_1595 at 1828.938 kb on + strand, within Shew_1595 at 1829.250 kb on + strand, within Shew_1596 at 1829.291 kb on + strand, within Shew_1596
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,827,352 + +0.4 1,827,427 + +1.2 1,827,442 + +0.4 1,827,450 - +1.3 1,827,713 - Shew_1594 0.35 +0.6 1,828,044 - Shew_1594 0.69 +1.1 1,828,102 - Shew_1594 0.75 +0.3 1,828,306 + +0.6 1,828,551 + Shew_1595 0.31 -0.0 1,828,701 - Shew_1595 0.53 +0.3 1,828,775 - Shew_1595 0.65 +0.2 1,828,900 - Shew_1595 0.84 -0.1 1,828,910 - Shew_1595 0.85 +0.1 1,828,938 + Shew_1595 0.89 -0.5 1,829,250 + Shew_1596 0.19 -0.2 1,829,291 + Shew_1596 0.22 -0.1
Or see this region's nucleotide sequence