Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1553 and Shew_1554 are separated by 135 nucleotides Shew_1554 and Shew_1555 are separated by 262 nucleotides Shew_1555 and Shew_1556 are separated by 256 nucleotides Shew_1556 and Shew_1557 are separated by 187 nucleotides
Shew_1553: Shew_1553 - hypothetical protein (RefSeq), at 1,784,052 to 1,784,534
_1553
Shew_1554: Shew_1554 - hypothetical protein (RefSeq), at 1,784,670 to 1,785,014
_1554
Shew_1555: Shew_1555 - Glu/Leu/Phe/Val dehydrogenase, dimerisation region (RefSeq), at 1,785,277 to 1,786,308
_1555
Shew_1556: Shew_1556 - hypothetical protein (RefSeq), at 1,786,565 to 1,787,014
_1556
Shew_1557: Shew_1557 - cupin 4 family protein (RefSeq), at 1,787,202 to 1,788,362
_1557
Position (kb) 1785
1786
1787 Strain fitness (log2 ratio) -1
0
1 at 1784.701 kb on - strand at 1784.870 kb on + strand, within Shew_1554 at 1785.040 kb on - strand at 1785.184 kb on - strand at 1785.801 kb on + strand, within Shew_1555 at 1785.966 kb on - strand, within Shew_1555 at 1787.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,784,701 - +0.7 1,784,870 + Shew_1554 0.58 -0.0 1,785,040 - -1.0 1,785,184 - +0.4 1,785,801 + Shew_1555 0.51 +0.9 1,785,966 - Shew_1555 0.67 +0.5 1,787,287 - +0.2
Or see this region's nucleotide sequence