Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1522 and Shew_1523 are separated by 158 nucleotides Shew_1523 and Shew_1524 overlap by 11 nucleotides Shew_1524 and Shew_1525 overlap by 1 nucleotides Shew_1525 and Shew_1526 are separated by 3 nucleotides
Shew_1522: Shew_1522 - prolyl oligopeptidase (RefSeq), at 1,745,372 to 1,747,462
_1522
Shew_1523: Shew_1523 - 4-hydroxybenzoyl-CoA thioesterase (RefSeq), at 1,747,621 to 1,748,019
_1523
Shew_1524: Shew_1524 - MotA/TolQ/ExbB proton channel (RefSeq), at 1,748,009 to 1,748,698
_1524
Shew_1525: Shew_1525 - biopolymer transport protein ExbD/TolR (RefSeq), at 1,748,698 to 1,749,132
_1525
Shew_1526: Shew_1526 - TolA family protein (RefSeq), at 1,749,136 to 1,750,071
_1526
Position (kb)
1748
1749 Strain fitness (log2 ratio)
-2
-1
0
1 at 1747.124 kb on + strand, within Shew_1522 at 1747.747 kb on - strand, within Shew_1523 at 1747.860 kb on + strand, within Shew_1523 at 1747.930 kb on - strand, within Shew_1523 at 1747.951 kb on + strand, within Shew_1523 at 1748.296 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.950 kb on + strand, within Shew_1525 at 1749.245 kb on - strand, within Shew_1526 at 1749.551 kb on - strand, within Shew_1526
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,747,124 + Shew_1522 0.84 -0.1 1,747,747 - Shew_1523 0.32 -2.2 1,747,860 + Shew_1523 0.60 -0.2 1,747,930 - Shew_1523 0.77 -1.1 1,747,951 + Shew_1523 0.83 -0.3 1,748,296 + Shew_1524 0.42 -0.6 1,748,437 + Shew_1524 0.62 -1.1 1,748,437 + Shew_1524 0.62 -0.1 1,748,950 + Shew_1525 0.58 -1.4 1,749,245 - Shew_1526 0.12 -0.8 1,749,551 - Shew_1526 0.44 -1.5
Or see this region's nucleotide sequence